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A compact and highly sensitive light-emitting diode-induced fluorescence detector for capillary flow systems.
Talanta. 2012 Jan 15; 88:463-7.T

Abstract

A compact and highly sensitive light-emitting diode-induced fluorescence detector (LED-FD) was constructed and evaluated. The LED excitation beam was restricted by a 200 μm diameter pinhole on a 55 μm thick lighttight film. The LED, excitation filter, pinhole, and capillary flow cell were placed tightly against each other without either optical lens or excitation fiber. The performance of the LED-FD was evaluated by flow injection analysis (FIA) of sodium fluorescein solution. The limit of detection (LOD) was determined as 0.75 nM (SNR=3). Compared with our previous work, a 3.5-fold enhancement on the signal-to-noise ratio (SNR) was obtained. The system exhibited linear responses in the concentration range of 2-200 nM (R=0.9993). The error of repeatability was within 2.0% R.S.D. on peak height in a ten-repeated injection of 5 nM solution. The reproducibility between devices was within 2.2% R.S.D. on peak height of 10nM fluorescein solution. Finally, the LED-FD was compared with commercial fluorescence detector, and similar fluorescence signal intensity was obtained.

Authors+Show Affiliations

Department of Instrumentation & Analytical Chemistry, Key Lab of Separation Science for Analytical Chemistry of CAS, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22265527

Citation

Geng, Xuhui, et al. "A Compact and Highly Sensitive Light-emitting Diode-induced Fluorescence Detector for Capillary Flow Systems." Talanta, vol. 88, 2012, pp. 463-7.
Geng X, Wu D, Guan Y. A compact and highly sensitive light-emitting diode-induced fluorescence detector for capillary flow systems. Talanta. 2012;88:463-7.
Geng, X., Wu, D., & Guan, Y. (2012). A compact and highly sensitive light-emitting diode-induced fluorescence detector for capillary flow systems. Talanta, 88, 463-7. https://doi.org/10.1016/j.talanta.2011.11.015
Geng X, Wu D, Guan Y. A Compact and Highly Sensitive Light-emitting Diode-induced Fluorescence Detector for Capillary Flow Systems. Talanta. 2012 Jan 15;88:463-7. PubMed PMID: 22265527.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A compact and highly sensitive light-emitting diode-induced fluorescence detector for capillary flow systems. AU - Geng,Xuhui, AU - Wu,Dapeng, AU - Guan,Yafeng, Y1 - 2011/11/09/ PY - 2011/07/19/received PY - 2011/11/02/revised PY - 2011/11/03/accepted PY - 2012/1/24/entrez PY - 2012/1/24/pubmed PY - 2012/6/5/medline SP - 463 EP - 7 JF - Talanta JO - Talanta VL - 88 N2 - A compact and highly sensitive light-emitting diode-induced fluorescence detector (LED-FD) was constructed and evaluated. The LED excitation beam was restricted by a 200 μm diameter pinhole on a 55 μm thick lighttight film. The LED, excitation filter, pinhole, and capillary flow cell were placed tightly against each other without either optical lens or excitation fiber. The performance of the LED-FD was evaluated by flow injection analysis (FIA) of sodium fluorescein solution. The limit of detection (LOD) was determined as 0.75 nM (SNR=3). Compared with our previous work, a 3.5-fold enhancement on the signal-to-noise ratio (SNR) was obtained. The system exhibited linear responses in the concentration range of 2-200 nM (R=0.9993). The error of repeatability was within 2.0% R.S.D. on peak height in a ten-repeated injection of 5 nM solution. The reproducibility between devices was within 2.2% R.S.D. on peak height of 10nM fluorescein solution. Finally, the LED-FD was compared with commercial fluorescence detector, and similar fluorescence signal intensity was obtained. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/22265527/A_compact_and_highly_sensitive_light_emitting_diode_induced_fluorescence_detector_for_capillary_flow_systems_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(11)00993-3 DB - PRIME DP - Unbound Medicine ER -