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On-line concentration and separation of cationic and anionic neurochemicals by capillary electrophoresis with UV absorption detection.
Talanta. 2012 Jan 15; 88:638-45.T

Abstract

This paper presents on-line simultaneous concentration and separation of cationic and anionic neurochemicals by capillary electrophoresis (CE) with UV absorbance spectroscopy. Neurochemical stacking exploits differences in local electric field and viscosity between the sample zone and the background electrolyte (BGE). To achieve these discontinuous conditions for CE, neurochemicals were prepared in a solution containing 1mM formic acid and 20% (v/v) acetonitrile (ACN). The capillary was filled with a solution of 500 mM Tris-borate (TB) and 10% (v/v) glycerol. The buffer vial contained 500 mM TB and 0.5% (v/v) polyethylene oxide (PEO). After injecting a large sample volume, PEO enters the capillary by electro-osmotic flow (EOF). Anionic neurochemicals stacked at the sample zone and PEO-containing BGE boundary. Simultaneously, cationic neurochemicals were concentrated at the boundary between the sample zone and the glycerol-containing BGE. The concentrated cationic neurochemicals were baseline separated in the presence of glycerol, mainly due to hydrogen bonding interactions between glycerol hydroxyl groups and the neurochemical's hydroxyl and amino groups. Under optimal stacking conditions, we observed the following: (a) the maximum sample injection volume was 720 nL; (b) the limit of detection for signal-to-noise ratio of 3 ranged from 14.7 to 313.4 nM; and (c) sensitivity enhancements compared to normal injection (15 nL) ranged from 116 to 281-fold. We evaluated the proposed method by the determination of neurochemicals in urine samples.

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Authors+Show Affiliations

Hsieh MM
Department of Chemistry, National Kaohsiung Normal University, No. 62, Shenjhong Rd., Yanchao District, Kaohsiung City 82446, Taiwan, R.O.C. t3644@nknucc.nknu.edu.tw
Lin EP
No affiliation info available
Huang SW
No affiliation info available

MeSH

BuffersChemical FractionationElectrophoresis, CapillaryGlycerolHydrogen BondingIndicanLimit of DetectionNormetanephrineOsmosisPhotoelectron SpectroscopyPolyethylene GlycolsSignal-To-Noise RatioSolutionsStatic ElectricityTryptaminesVanilmandelic AcidViscosity

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22265552

Citation

Hsieh, Ming-Mu, et al. "On-line Concentration and Separation of Cationic and Anionic Neurochemicals By Capillary Electrophoresis With UV Absorption Detection." Talanta, vol. 88, 2012, pp. 638-45.
Hsieh MM, Lin EP, Huang SW. On-line concentration and separation of cationic and anionic neurochemicals by capillary electrophoresis with UV absorption detection. Talanta. 2012;88:638-45.
Hsieh, M. M., Lin, E. P., & Huang, S. W. (2012). On-line concentration and separation of cationic and anionic neurochemicals by capillary electrophoresis with UV absorption detection. Talanta, 88, 638-45. https://doi.org/10.1016/j.talanta.2011.11.050
Hsieh MM, Lin EP, Huang SW. On-line Concentration and Separation of Cationic and Anionic Neurochemicals By Capillary Electrophoresis With UV Absorption Detection. Talanta. 2012 Jan 15;88:638-45. PubMed PMID: 22265552.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - On-line concentration and separation of cationic and anionic neurochemicals by capillary electrophoresis with UV absorption detection. AU - Hsieh,Ming-Mu, AU - Lin,En-Ping, AU - Huang,Shiou-Wen, Y1 - 2011/11/25/ PY - 2011/09/05/received PY - 2011/11/16/revised PY - 2011/11/17/accepted PY - 2012/1/24/entrez PY - 2012/1/24/pubmed PY - 2012/6/5/medline SP - 638 EP - 45 JF - Talanta JO - Talanta VL - 88 N2 - This paper presents on-line simultaneous concentration and separation of cationic and anionic neurochemicals by capillary electrophoresis (CE) with UV absorbance spectroscopy. Neurochemical stacking exploits differences in local electric field and viscosity between the sample zone and the background electrolyte (BGE). To achieve these discontinuous conditions for CE, neurochemicals were prepared in a solution containing 1mM formic acid and 20% (v/v) acetonitrile (ACN). The capillary was filled with a solution of 500 mM Tris-borate (TB) and 10% (v/v) glycerol. The buffer vial contained 500 mM TB and 0.5% (v/v) polyethylene oxide (PEO). After injecting a large sample volume, PEO enters the capillary by electro-osmotic flow (EOF). Anionic neurochemicals stacked at the sample zone and PEO-containing BGE boundary. Simultaneously, cationic neurochemicals were concentrated at the boundary between the sample zone and the glycerol-containing BGE. The concentrated cationic neurochemicals were baseline separated in the presence of glycerol, mainly due to hydrogen bonding interactions between glycerol hydroxyl groups and the neurochemical's hydroxyl and amino groups. Under optimal stacking conditions, we observed the following: (a) the maximum sample injection volume was 720 nL; (b) the limit of detection for signal-to-noise ratio of 3 ranged from 14.7 to 313.4 nM; and (c) sensitivity enhancements compared to normal injection (15 nL) ranged from 116 to 281-fold. We evaluated the proposed method by the determination of neurochemicals in urine samples. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/22265552/On_line_concentration_and_separation_of_cationic_and_anionic_neurochemicals_by_capillary_electrophoresis_with_UV_absorption_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(11)01032-0 DB - PRIME DP - Unbound Medicine ER -