Tags

Type your tag names separated by a space and hit enter

Synthesis of mixed coating with multi-functional groups for in-tube hollow fiber solid phase microextraction-high performance liquid chromatography-inductively coupled plasma mass spectrometry speciation of arsenic in human urine.
J Chromatogr A. 2012 Mar 02; 1227:19-28.JC

Abstract

A novel method based on in-tube hollow fiber-solid phase microextraction (in-tube HF-SPME) on-line coupled with ion pair reversed phase high performance liquid chromatography (IP-RP-HPLC)-inductively coupled plasma mass spectrometry (ICP-MS) was developed for arsenic speciation. Partially sulfonated poly(styrene) (PSP) and mixed-sol of 3-mercapto propyltrimethoxysilane (MPTS) and N-(2-aminoethyl)-3-aminopropyltrimethoxysilane (AAPTS) were prepared and immobilized in the pores and the inner surface of polypropylene hollow fiber (HF). The prepared MPTS-AAPTS/PSP immobilized HF was characterized by FT-IR spectroscopy and scanning electron microscope (SEM). With arsenite (As(III)), arsenate (As(V)), monomethylarsonic acid (MMA), dimethylarsenic acid (DMA), arsenobetaine (AsB) and arsenocholine (AsC) as model arsenic species, a series of factors that influence the extraction of target arsenic species by in-tube HF-SPME, including pH value, sample volume and flow rate, elution conditions and interference of co-existing ions were investigated in details, and the conditions for subsequent HPLC-ICP-MS determination were also optimized. Under the optimal conditions, the sampling frequency was 6.5 h⁻¹, the detection limits for six target arsenic species were in the range of 0.017-0.053 μg L⁻¹ with the relative standard deviations (c(As(V),MMA)=0.1 μg L⁻¹, c(As(III),DMA,AsB,AsC)=0.5 μg L⁻¹, n=5) ranging in 3.1-8.7%, and the enrichment factors were varied from 4 to 19-fold. To validate the accuracy of this method, certified reference materials DORM-2 (dogfish) and CRM No. 18 (human urine) were analyzed, and the determined values were in good agreement with the certified values. The proposed method was also successfully applied for arsenic speciation in human urine samples, and the recoveries for the spiked samples were in the range of 92.6-107%. The self-designed in-tube HF-SPME-HPLC-ICP-MS system shows high efficiency and good stability, and the proposed method is sensitive and suitable for simultaneous speciation of organic and inorganic arsenic species (including anions and cations) in biological samples.

Authors+Show Affiliations

Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22265781

Citation

Chen, Beibei, et al. "Synthesis of Mixed Coating With Multi-functional Groups for In-tube Hollow Fiber Solid Phase Microextraction-high Performance Liquid Chromatography-inductively Coupled Plasma Mass Spectrometry Speciation of Arsenic in Human Urine." Journal of Chromatography. A, vol. 1227, 2012, pp. 19-28.
Chen B, Hu B, He M, et al. Synthesis of mixed coating with multi-functional groups for in-tube hollow fiber solid phase microextraction-high performance liquid chromatography-inductively coupled plasma mass spectrometry speciation of arsenic in human urine. J Chromatogr A. 2012;1227:19-28.
Chen, B., Hu, B., He, M., Mao, X., & Zu, W. (2012). Synthesis of mixed coating with multi-functional groups for in-tube hollow fiber solid phase microextraction-high performance liquid chromatography-inductively coupled plasma mass spectrometry speciation of arsenic in human urine. Journal of Chromatography. A, 1227, 19-28. https://doi.org/10.1016/j.chroma.2011.12.086
Chen B, et al. Synthesis of Mixed Coating With Multi-functional Groups for In-tube Hollow Fiber Solid Phase Microextraction-high Performance Liquid Chromatography-inductively Coupled Plasma Mass Spectrometry Speciation of Arsenic in Human Urine. J Chromatogr A. 2012 Mar 2;1227:19-28. PubMed PMID: 22265781.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Synthesis of mixed coating with multi-functional groups for in-tube hollow fiber solid phase microextraction-high performance liquid chromatography-inductively coupled plasma mass spectrometry speciation of arsenic in human urine. AU - Chen,Beibei, AU - Hu,Bin, AU - He,Man, AU - Mao,Xiangju, AU - Zu,Wanqing, Y1 - 2012/01/08/ PY - 2011/11/05/received PY - 2011/12/22/revised PY - 2011/12/23/accepted PY - 2012/1/24/entrez PY - 2012/1/24/pubmed PY - 2012/4/20/medline SP - 19 EP - 28 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1227 N2 - A novel method based on in-tube hollow fiber-solid phase microextraction (in-tube HF-SPME) on-line coupled with ion pair reversed phase high performance liquid chromatography (IP-RP-HPLC)-inductively coupled plasma mass spectrometry (ICP-MS) was developed for arsenic speciation. Partially sulfonated poly(styrene) (PSP) and mixed-sol of 3-mercapto propyltrimethoxysilane (MPTS) and N-(2-aminoethyl)-3-aminopropyltrimethoxysilane (AAPTS) were prepared and immobilized in the pores and the inner surface of polypropylene hollow fiber (HF). The prepared MPTS-AAPTS/PSP immobilized HF was characterized by FT-IR spectroscopy and scanning electron microscope (SEM). With arsenite (As(III)), arsenate (As(V)), monomethylarsonic acid (MMA), dimethylarsenic acid (DMA), arsenobetaine (AsB) and arsenocholine (AsC) as model arsenic species, a series of factors that influence the extraction of target arsenic species by in-tube HF-SPME, including pH value, sample volume and flow rate, elution conditions and interference of co-existing ions were investigated in details, and the conditions for subsequent HPLC-ICP-MS determination were also optimized. Under the optimal conditions, the sampling frequency was 6.5 h⁻¹, the detection limits for six target arsenic species were in the range of 0.017-0.053 μg L⁻¹ with the relative standard deviations (c(As(V),MMA)=0.1 μg L⁻¹, c(As(III),DMA,AsB,AsC)=0.5 μg L⁻¹, n=5) ranging in 3.1-8.7%, and the enrichment factors were varied from 4 to 19-fold. To validate the accuracy of this method, certified reference materials DORM-2 (dogfish) and CRM No. 18 (human urine) were analyzed, and the determined values were in good agreement with the certified values. The proposed method was also successfully applied for arsenic speciation in human urine samples, and the recoveries for the spiked samples were in the range of 92.6-107%. The self-designed in-tube HF-SPME-HPLC-ICP-MS system shows high efficiency and good stability, and the proposed method is sensitive and suitable for simultaneous speciation of organic and inorganic arsenic species (including anions and cations) in biological samples. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/22265781/Synthesis_of_mixed_coating_with_multi_functional_groups_for_in_tube_hollow_fiber_solid_phase_microextraction_high_performance_liquid_chromatography_inductively_coupled_plasma_mass_spectrometry_speciation_of_arsenic_in_human_urine_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(12)00010-6 DB - PRIME DP - Unbound Medicine ER -