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Determination of the absolute configuration of peptide natural products by using stable isotope labeling and mass spectrometry.
Chemistry. 2012 Feb 20; 18(8):2342-8.C

Abstract

Structure elucidation of natural products including the absolute configuration is a complex task that involves different analytical methods like mass spectrometry, NMR spectroscopy, and chemical derivation, which are usually performed after the isolation of the compound of interest. Here, a combination of stable isotope labeling of Photorhabdus and Xenorhabdus strains and their transaminase mutants followed by detailed MS analysis enabled the structure elucidation of novel cyclopeptides named GameXPeptides including their absolute configuration in crude extracts without their actual isolation.

Authors+Show Affiliations

Institut für Molekulare Biowissenschaften, Molekulare Biotechnologie, Goethe Universität Frankfurt, Max-von-Laue-Strasse 9, 60436 Frankfurt am Main, Germany. h.bode@bio.uni-frankfurt.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22266804

Citation

Bode, Helge B., et al. "Determination of the Absolute Configuration of Peptide Natural Products By Using Stable Isotope Labeling and Mass Spectrometry." Chemistry (Weinheim an Der Bergstrasse, Germany), vol. 18, no. 8, 2012, pp. 2342-8.
Bode HB, Reimer D, Fuchs SW, et al. Determination of the absolute configuration of peptide natural products by using stable isotope labeling and mass spectrometry. Chemistry. 2012;18(8):2342-8.
Bode, H. B., Reimer, D., Fuchs, S. W., Kirchner, F., Dauth, C., Kegler, C., Lorenzen, W., Brachmann, A. O., & Grün, P. (2012). Determination of the absolute configuration of peptide natural products by using stable isotope labeling and mass spectrometry. Chemistry (Weinheim an Der Bergstrasse, Germany), 18(8), 2342-8. https://doi.org/10.1002/chem.201103479
Bode HB, et al. Determination of the Absolute Configuration of Peptide Natural Products By Using Stable Isotope Labeling and Mass Spectrometry. Chemistry. 2012 Feb 20;18(8):2342-8. PubMed PMID: 22266804.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of the absolute configuration of peptide natural products by using stable isotope labeling and mass spectrometry. AU - Bode,Helge B, AU - Reimer,Daniela, AU - Fuchs,Sebastian W, AU - Kirchner,Ferdinand, AU - Dauth,Christina, AU - Kegler,Carsten, AU - Lorenzen,Wolfram, AU - Brachmann,Alexander O, AU - Grün,Peter, Y1 - 2012/01/20/ PY - 2011/11/04/received PY - 2012/1/24/entrez PY - 2012/1/24/pubmed PY - 2012/5/10/medline SP - 2342 EP - 8 JF - Chemistry (Weinheim an der Bergstrasse, Germany) JO - Chemistry VL - 18 IS - 8 N2 - Structure elucidation of natural products including the absolute configuration is a complex task that involves different analytical methods like mass spectrometry, NMR spectroscopy, and chemical derivation, which are usually performed after the isolation of the compound of interest. Here, a combination of stable isotope labeling of Photorhabdus and Xenorhabdus strains and their transaminase mutants followed by detailed MS analysis enabled the structure elucidation of novel cyclopeptides named GameXPeptides including their absolute configuration in crude extracts without their actual isolation. SN - 1521-3765 UR - https://www.unboundmedicine.com/medline/citation/22266804/Determination_of_the_absolute_configuration_of_peptide_natural_products_by_using_stable_isotope_labeling_and_mass_spectrometry_ L2 - https://doi.org/10.1002/chem.201103479 DB - PRIME DP - Unbound Medicine ER -