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D-pinitol inhibits RANKL-induced osteoclastogenesis.
Int Immunopharmacol. 2012 Mar; 12(3):494-500.II

Abstract

Numerous studies have indicated that inflammatory cytokines play a major role in osteoclastogenesis, leading to the bone resorption that is frequently associated with osteoporosis. D-pinitol, a 3-methoxy analogue of D-chiroinositol, was identified as an active principle in soy foods and legumes. Here we found that D-pinitol markedly inhibited the receptor activator of nuclear factor kappa B ligand (RANKL)-induced osteoclastic differentiation from bone marrow stromal cells and RAW264.7 macrophage cells. In addition, D-pinitol also reduced RANKL-induced p38 and JNK phosphorylation. Furthermore, RANKL-mediated increase of IKK, IκBα, and p65 phosphorylation and NF-κB-luciferase activity was inhibited by D-pinitol. However, D-pinitol did not affect the proliferation and differentiation of osteoblasts. In addition, D-pinitol also prevented the bone loss induced by ovariectomy in vivo. Our data suggest that D-pinitol inhibits osteoclastogenesis from bone marrow stromal cells and macrophage cells via attenuated RANKL-induced p38, JNK, and NF-κB activation, which in turn protect bone loss from ovariectomy.

Authors+Show Affiliations

Institute of Biomedical Sciences, National Chung Hsing University, Taichung, Taiwan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22269833

Citation

Liu, Shan-Chi, et al. "D-pinitol Inhibits RANKL-induced Osteoclastogenesis." International Immunopharmacology, vol. 12, no. 3, 2012, pp. 494-500.
Liu SC, Chuang SM, Tang CH. D-pinitol inhibits RANKL-induced osteoclastogenesis. Int Immunopharmacol. 2012;12(3):494-500.
Liu, S. C., Chuang, S. M., & Tang, C. H. (2012). D-pinitol inhibits RANKL-induced osteoclastogenesis. International Immunopharmacology, 12(3), 494-500. https://doi.org/10.1016/j.intimp.2012.01.002
Liu SC, Chuang SM, Tang CH. D-pinitol Inhibits RANKL-induced Osteoclastogenesis. Int Immunopharmacol. 2012;12(3):494-500. PubMed PMID: 22269833.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - D-pinitol inhibits RANKL-induced osteoclastogenesis. AU - Liu,Shan-Chi, AU - Chuang,Show-Mei, AU - Tang,Chih-Hsin, Y1 - 2012/01/23/ PY - 2011/10/26/received PY - 2012/01/02/revised PY - 2012/01/03/accepted PY - 2012/1/25/entrez PY - 2012/1/25/pubmed PY - 2012/6/13/medline SP - 494 EP - 500 JF - International immunopharmacology JO - Int Immunopharmacol VL - 12 IS - 3 N2 - Numerous studies have indicated that inflammatory cytokines play a major role in osteoclastogenesis, leading to the bone resorption that is frequently associated with osteoporosis. D-pinitol, a 3-methoxy analogue of D-chiroinositol, was identified as an active principle in soy foods and legumes. Here we found that D-pinitol markedly inhibited the receptor activator of nuclear factor kappa B ligand (RANKL)-induced osteoclastic differentiation from bone marrow stromal cells and RAW264.7 macrophage cells. In addition, D-pinitol also reduced RANKL-induced p38 and JNK phosphorylation. Furthermore, RANKL-mediated increase of IKK, IκBα, and p65 phosphorylation and NF-κB-luciferase activity was inhibited by D-pinitol. However, D-pinitol did not affect the proliferation and differentiation of osteoblasts. In addition, D-pinitol also prevented the bone loss induced by ovariectomy in vivo. Our data suggest that D-pinitol inhibits osteoclastogenesis from bone marrow stromal cells and macrophage cells via attenuated RANKL-induced p38, JNK, and NF-κB activation, which in turn protect bone loss from ovariectomy. SN - 1878-1705 UR - https://www.unboundmedicine.com/medline/citation/22269833/D_pinitol_inhibits_RANKL_induced_osteoclastogenesis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-5769(12)00011-2 DB - PRIME DP - Unbound Medicine ER -