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Cannabinoid disposition in oral fluid after controlled smoked cannabis.
Clin Chem. 2012 Apr; 58(4):748-56.CC

Abstract

BACKGROUND

We measured Δ(9)-tetrahydrocannabinol (THC), 11-nor-9-carboxy-THC (THCCOOH), cannabidiol (CBD), and cannabinol (CBN) disposition in oral fluid (OF) following controlled cannabis smoking to evaluate whether monitoring multiple cannabinoids in OF improved OF test interpretation.

METHODS

Cannabis smokers provided written informed consent for this institutional review board-approved study. OF was collected with the Quantisal™ device following ad libitum smoking of one 6.8% THC cigarette. Cannabinoids were quantified by 2-dimensional GC-MS. We evaluated 8 alternative cutoffs based on different drug testing program needs.

RESULTS

10 participants provided 86 OF samples -0.5 h before and 0.25, 0.5, 1, 2, 3, 4, 6, and 22 h after initiation of smoking. Before smoking, OF samples of 4 and 9 participants were positive for THC and THCCOOH, respectively, but none were positive for CBD and CBN. Maximum THC, CBD, and CBN concentrations occurred within 0.5 h, with medians of 644, 30.4, and 49.0 μg/L, respectively. All samples were THC positive at 6 h (2.1-44.4 μg/L), and 4 of 6 were positive at 22 h. CBD and CBN were positive only up to 6 h in 3 (0.6-2.1 μg/L) and 4 (1.0-4.4 μg/L) participants, respectively. The median maximum THCCOOH OF concentration was 115 ng/L, with all samples positive to 6 h (14.8-263 ng/L) and 5 of 6 positive at 22 h.

CONCLUSIONS

By quantifying multiple cannabinoids and evaluating different analytical cutoffs after controlled cannabis smoking, we determined windows of drug detection, found suggested markers of recent smoking, and minimized the potential for passive contamination.

Authors+Show Affiliations

Chemistry and Drug Metabolism, Intramural Research Program, National Institute on Drug Abuse, NIH, Baltimore, MD 21224, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Intramural

Language

eng

PubMed ID

22273566

Citation

Lee, Dayong, et al. "Cannabinoid Disposition in Oral Fluid After Controlled Smoked Cannabis." Clinical Chemistry, vol. 58, no. 4, 2012, pp. 748-56.
Lee D, Schwope DM, Milman G, et al. Cannabinoid disposition in oral fluid after controlled smoked cannabis. Clin Chem. 2012;58(4):748-56.
Lee, D., Schwope, D. M., Milman, G., Barnes, A. J., Gorelick, D. A., & Huestis, M. A. (2012). Cannabinoid disposition in oral fluid after controlled smoked cannabis. Clinical Chemistry, 58(4), 748-56. https://doi.org/10.1373/clinchem.2011.177881
Lee D, et al. Cannabinoid Disposition in Oral Fluid After Controlled Smoked Cannabis. Clin Chem. 2012;58(4):748-56. PubMed PMID: 22273566.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cannabinoid disposition in oral fluid after controlled smoked cannabis. AU - Lee,Dayong, AU - Schwope,David M, AU - Milman,Garry, AU - Barnes,Allan J, AU - Gorelick,David A, AU - Huestis,Marilyn A, Y1 - 2012/01/24/ PY - 2012/1/26/entrez PY - 2012/1/26/pubmed PY - 2012/6/9/medline SP - 748 EP - 56 JF - Clinical chemistry JO - Clin Chem VL - 58 IS - 4 N2 - BACKGROUND: We measured Δ(9)-tetrahydrocannabinol (THC), 11-nor-9-carboxy-THC (THCCOOH), cannabidiol (CBD), and cannabinol (CBN) disposition in oral fluid (OF) following controlled cannabis smoking to evaluate whether monitoring multiple cannabinoids in OF improved OF test interpretation. METHODS: Cannabis smokers provided written informed consent for this institutional review board-approved study. OF was collected with the Quantisal™ device following ad libitum smoking of one 6.8% THC cigarette. Cannabinoids were quantified by 2-dimensional GC-MS. We evaluated 8 alternative cutoffs based on different drug testing program needs. RESULTS: 10 participants provided 86 OF samples -0.5 h before and 0.25, 0.5, 1, 2, 3, 4, 6, and 22 h after initiation of smoking. Before smoking, OF samples of 4 and 9 participants were positive for THC and THCCOOH, respectively, but none were positive for CBD and CBN. Maximum THC, CBD, and CBN concentrations occurred within 0.5 h, with medians of 644, 30.4, and 49.0 μg/L, respectively. All samples were THC positive at 6 h (2.1-44.4 μg/L), and 4 of 6 were positive at 22 h. CBD and CBN were positive only up to 6 h in 3 (0.6-2.1 μg/L) and 4 (1.0-4.4 μg/L) participants, respectively. The median maximum THCCOOH OF concentration was 115 ng/L, with all samples positive to 6 h (14.8-263 ng/L) and 5 of 6 positive at 22 h. CONCLUSIONS: By quantifying multiple cannabinoids and evaluating different analytical cutoffs after controlled cannabis smoking, we determined windows of drug detection, found suggested markers of recent smoking, and minimized the potential for passive contamination. SN - 1530-8561 UR - https://www.unboundmedicine.com/medline/citation/22273566/Cannabinoid_disposition_in_oral_fluid_after_controlled_smoked_cannabis_ L2 - https://academic.oup.com/clinchem/article-lookup/doi/10.1373/clinchem.2011.177881 DB - PRIME DP - Unbound Medicine ER -