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Surface-enhanced Raman scattering detection of DNAs derived from virus genomes using Au-coated paramagnetic nanoparticles.
Langmuir. 2012 Feb 28; 28(8):4030-7.L

Abstract

A magnetic capture-based, surface-enhanced Raman scattering (SERS) assay for DNA detection has been developed which utilizes Au-coated paramagnetic nanoparticles (Au@PMPs) as both a SERS substrate and effective bioseparation reagent for the selective removal of target DNAs from solution. Hybridization reactions contained two target DNAs, sequence complementary reporter probes conjugated with spectrally distinct Raman dyes distinct for each target, and Au@PMPs conjugated with sequence complementary capture probes. In this case, target DNAs were derived from the RNA genomes of the Rift Valley Fever virus (RVFV) or West Nile virus (WNV). The hybridization reactions were incubated for a short period and then concentrated within the focus beam of an interrogating laser by magnetic pull-down. The attendant SERS response of each individually captured DNA provided a limit of detection sensitivity in the range 20-100 nM. X-ray diffraction and UV-vis analysis validated both the desired surface plasmon resonance properties and bimetallic composition of synthesized Au@PMPs, and UV-vis spectroscopy confirmed conjugation of the Raman dye compounds malachite green (MG) and erythrosin B (EB) with the RVFV and WNV reporter probes, respectively. Finally, hybridization reactions assembled for multiplexed detection of both targets yielded mixed MG/EB spectra and clearly differentiated peaks which facilitate the quantitative detection of each DNA target. On the basis of the simple design of a single-particle DNA detection assay, the opportunity is provided to develop magnetic capture-based SERS assays that are easily assembled and adapted for high-level multiplex detection using low-cost Raman instrumentation.

Authors+Show Affiliations

Department of Chemical and Petroleum Engineering, University of Wyoming, Laramie, Wyoming 82071, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

22276995

Citation

Zhang, Hao, et al. "Surface-enhanced Raman Scattering Detection of DNAs Derived From Virus Genomes Using Au-coated Paramagnetic Nanoparticles." Langmuir : the ACS Journal of Surfaces and Colloids, vol. 28, no. 8, 2012, pp. 4030-7.
Zhang H, Harpster MH, Wilson WC, et al. Surface-enhanced Raman scattering detection of DNAs derived from virus genomes using Au-coated paramagnetic nanoparticles. Langmuir. 2012;28(8):4030-7.
Zhang, H., Harpster, M. H., Wilson, W. C., & Johnson, P. A. (2012). Surface-enhanced Raman scattering detection of DNAs derived from virus genomes using Au-coated paramagnetic nanoparticles. Langmuir : the ACS Journal of Surfaces and Colloids, 28(8), 4030-7. https://doi.org/10.1021/la204890t
Zhang H, et al. Surface-enhanced Raman Scattering Detection of DNAs Derived From Virus Genomes Using Au-coated Paramagnetic Nanoparticles. Langmuir. 2012 Feb 28;28(8):4030-7. PubMed PMID: 22276995.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Surface-enhanced Raman scattering detection of DNAs derived from virus genomes using Au-coated paramagnetic nanoparticles. AU - Zhang,Hao, AU - Harpster,Mark H, AU - Wilson,William C, AU - Johnson,Patrick A, Y1 - 2012/02/15/ PY - 2012/1/27/entrez PY - 2012/1/27/pubmed PY - 2012/6/14/medline SP - 4030 EP - 7 JF - Langmuir : the ACS journal of surfaces and colloids JO - Langmuir VL - 28 IS - 8 N2 - A magnetic capture-based, surface-enhanced Raman scattering (SERS) assay for DNA detection has been developed which utilizes Au-coated paramagnetic nanoparticles (Au@PMPs) as both a SERS substrate and effective bioseparation reagent for the selective removal of target DNAs from solution. Hybridization reactions contained two target DNAs, sequence complementary reporter probes conjugated with spectrally distinct Raman dyes distinct for each target, and Au@PMPs conjugated with sequence complementary capture probes. In this case, target DNAs were derived from the RNA genomes of the Rift Valley Fever virus (RVFV) or West Nile virus (WNV). The hybridization reactions were incubated for a short period and then concentrated within the focus beam of an interrogating laser by magnetic pull-down. The attendant SERS response of each individually captured DNA provided a limit of detection sensitivity in the range 20-100 nM. X-ray diffraction and UV-vis analysis validated both the desired surface plasmon resonance properties and bimetallic composition of synthesized Au@PMPs, and UV-vis spectroscopy confirmed conjugation of the Raman dye compounds malachite green (MG) and erythrosin B (EB) with the RVFV and WNV reporter probes, respectively. Finally, hybridization reactions assembled for multiplexed detection of both targets yielded mixed MG/EB spectra and clearly differentiated peaks which facilitate the quantitative detection of each DNA target. On the basis of the simple design of a single-particle DNA detection assay, the opportunity is provided to develop magnetic capture-based SERS assays that are easily assembled and adapted for high-level multiplex detection using low-cost Raman instrumentation. SN - 1520-5827 UR - https://www.unboundmedicine.com/medline/citation/22276995/Surface_enhanced_Raman_scattering_detection_of_DNAs_derived_from_virus_genomes_using_Au_coated_paramagnetic_nanoparticles_ L2 - https://doi.org/10.1021/la204890t DB - PRIME DP - Unbound Medicine ER -