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Evaluation of Coccidioides antigen detection in dogs with coccidioidomycosis.
Clin Vaccine Immunol. 2012 Mar; 19(3):343-5.CV

Abstract

Antigen detection has been reported to be a promising method for rapid diagnosis of coccidioidomycosis in humans. Coccidioides antigen detection has not been previously reported in dogs with coccidioidomycosis and was evaluated in 60 cases diagnosed based on detection of anti-Coccidioides antibodies at titers of 1:16 or more in serum. Controls included dogs with presumed histoplasmosis or blastomycosis, other fungal infections, or nonfungal diseases and healthy dogs. Urine and serum specimens were tested using an enzyme immunoassay for Coccidioides galactomannan antigen. Antibody testing was performed at commercial veterinary reference laboratories. Antigen was detected in urine or serum of 12 of 60 (20.0%), urine only in 2 of 57 (3.5%), and serum only in 11 of 58 (19.0%) dogs with coccidioidomycosis. Antigen was detected in the urine of 3 of 43 (7.0%) and serum of 1 of 37 (2.7%) dogs with histoplasmosis or blastomycosis but not in 13 dogs with other fungal infections (serum, 9; urine, 13), 41 dogs with nonfungal diseases (urine, 41; serum, 18), or healthy dogs (serum, 21; urine, 21). Detection of antigen was an insensitive method for diagnosis of coccidioidomycosis in dogs in which the diagnosis was based primarily upon detection of antibodies at titers of 1:16 or higher, and the highest sensitivity was in serum.

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Authors+Show Affiliations

Kirsch EJ
MiraVista Diagnostics, Indianapolis, Indiana, USA. ekirsch@miravistalabs.com
Greene RT
No affiliation info available
Prahl A
No affiliation info available
Rubin SI
No affiliation info available
Sykes JE
No affiliation info available
Durkin MM
No affiliation info available
Wheat LJ
No affiliation info available

MeSH

AnimalsAntibodies, FungalAntigens, FungalCoccidioidesCoccidioidomycosisDog DiseasesDogsImmunoenzyme TechniquesMannansSensitivity and SpecificitySerumUrine

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22278324

Citation

Kirsch, Emily J., et al. "Evaluation of Coccidioides Antigen Detection in Dogs With Coccidioidomycosis." Clinical and Vaccine Immunology : CVI, vol. 19, no. 3, 2012, pp. 343-5.
Kirsch EJ, Greene RT, Prahl A, et al. Evaluation of Coccidioides antigen detection in dogs with coccidioidomycosis. Clin Vaccine Immunol. 2012;19(3):343-5.
Kirsch, E. J., Greene, R. T., Prahl, A., Rubin, S. I., Sykes, J. E., Durkin, M. M., & Wheat, L. J. (2012). Evaluation of Coccidioides antigen detection in dogs with coccidioidomycosis. Clinical and Vaccine Immunology : CVI, 19(3), 343-5. https://doi.org/10.1128/CVI.05631-11
Kirsch EJ, et al. Evaluation of Coccidioides Antigen Detection in Dogs With Coccidioidomycosis. Clin Vaccine Immunol. 2012;19(3):343-5. PubMed PMID: 22278324.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of Coccidioides antigen detection in dogs with coccidioidomycosis. AU - Kirsch,Emily J, AU - Greene,Russell T, AU - Prahl,Annalisa, AU - Rubin,Stanley I, AU - Sykes,Jane E, AU - Durkin,Michelle M, AU - Wheat,Lawrence J, Y1 - 2012/01/25/ PY - 2012/1/27/entrez PY - 2012/1/27/pubmed PY - 2012/6/14/medline SP - 343 EP - 5 JF - Clinical and vaccine immunology : CVI JO - Clin Vaccine Immunol VL - 19 IS - 3 N2 - Antigen detection has been reported to be a promising method for rapid diagnosis of coccidioidomycosis in humans. Coccidioides antigen detection has not been previously reported in dogs with coccidioidomycosis and was evaluated in 60 cases diagnosed based on detection of anti-Coccidioides antibodies at titers of 1:16 or more in serum. Controls included dogs with presumed histoplasmosis or blastomycosis, other fungal infections, or nonfungal diseases and healthy dogs. Urine and serum specimens were tested using an enzyme immunoassay for Coccidioides galactomannan antigen. Antibody testing was performed at commercial veterinary reference laboratories. Antigen was detected in urine or serum of 12 of 60 (20.0%), urine only in 2 of 57 (3.5%), and serum only in 11 of 58 (19.0%) dogs with coccidioidomycosis. Antigen was detected in the urine of 3 of 43 (7.0%) and serum of 1 of 37 (2.7%) dogs with histoplasmosis or blastomycosis but not in 13 dogs with other fungal infections (serum, 9; urine, 13), 41 dogs with nonfungal diseases (urine, 41; serum, 18), or healthy dogs (serum, 21; urine, 21). Detection of antigen was an insensitive method for diagnosis of coccidioidomycosis in dogs in which the diagnosis was based primarily upon detection of antibodies at titers of 1:16 or higher, and the highest sensitivity was in serum. SN - 1556-679X UR - https://www.unboundmedicine.com/medline/citation/22278324/Evaluation_of_Coccidioides_antigen_detection_in_dogs_with_coccidioidomycosis_ L2 - https://journals.asm.org/doi/10.1128/CVI.05631-11?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -