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Qualitative and quantitative analysis of the major phenolic compounds as antioxidants in barley and flaxseed hulls using HPLC/MS/MS.
J Sci Food Agric. 2012 Aug 15; 92(10):2062-8.JS

Abstract

BACKGROUND

Both qualitative and quantitative analyses of the major phenolic compounds in barley and flaxseed hulls were conducted using reverse phase high-performance liquid chromatography coupled with photodiode array detection and quadrupole time-of-flight mass spectrometry.

RESULTS

Ferulic acid, p-coumaric acid, vanillic acid and vanillin were identified and quantified in four barley hull samples. Four ferulate dehydrodimers were also detected. The phenolic compounds of flaxseed hull were distinct from those of barley hull. Three flaxseed hull samples varied significantly (P < 0.05) in their contents of secoisolariciresinol diglucoside (16.38-33.92 g kg(-1)), coumaric acid glucoside (35.68-49.22 g kg(-1)) and ferulic acid glucoside (5.07-15.23 g kg(-1)). The phytochemical profiles of co-extracts featured the major phenolic compounds from both barley and flaxseed hulls. The total phenolic content and 2,2-diphenyl-1-picrylhydrazyl radical-scavenging capacity varied significantly (P < 0.05) among different varieties of flaxseed and barley hulls.

CONCLUSION

As agricultural by-products, barley and flaxseed hulls may be utilised as potential sources of functional food ingredients through extraction and concentration of the phytochemicals identified above.

Authors+Show Affiliations

Department of Food Science, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22278383

Citation

Hao, Meili, and Trust Beta. "Qualitative and Quantitative Analysis of the Major Phenolic Compounds as Antioxidants in Barley and Flaxseed Hulls Using HPLC/MS/MS." Journal of the Science of Food and Agriculture, vol. 92, no. 10, 2012, pp. 2062-8.
Hao M, Beta T. Qualitative and quantitative analysis of the major phenolic compounds as antioxidants in barley and flaxseed hulls using HPLC/MS/MS. J Sci Food Agric. 2012;92(10):2062-8.
Hao, M., & Beta, T. (2012). Qualitative and quantitative analysis of the major phenolic compounds as antioxidants in barley and flaxseed hulls using HPLC/MS/MS. Journal of the Science of Food and Agriculture, 92(10), 2062-8. https://doi.org/10.1002/jsfa.5582
Hao M, Beta T. Qualitative and Quantitative Analysis of the Major Phenolic Compounds as Antioxidants in Barley and Flaxseed Hulls Using HPLC/MS/MS. J Sci Food Agric. 2012 Aug 15;92(10):2062-8. PubMed PMID: 22278383.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Qualitative and quantitative analysis of the major phenolic compounds as antioxidants in barley and flaxseed hulls using HPLC/MS/MS. AU - Hao,Meili, AU - Beta,Trust, Y1 - 2012/01/25/ PY - 2011/07/15/received PY - 2011/11/30/revised PY - 2011/12/02/accepted PY - 2012/1/27/entrez PY - 2012/1/27/pubmed PY - 2012/10/12/medline SP - 2062 EP - 8 JF - Journal of the science of food and agriculture JO - J. Sci. Food Agric. VL - 92 IS - 10 N2 - BACKGROUND: Both qualitative and quantitative analyses of the major phenolic compounds in barley and flaxseed hulls were conducted using reverse phase high-performance liquid chromatography coupled with photodiode array detection and quadrupole time-of-flight mass spectrometry. RESULTS: Ferulic acid, p-coumaric acid, vanillic acid and vanillin were identified and quantified in four barley hull samples. Four ferulate dehydrodimers were also detected. The phenolic compounds of flaxseed hull were distinct from those of barley hull. Three flaxseed hull samples varied significantly (P < 0.05) in their contents of secoisolariciresinol diglucoside (16.38-33.92 g kg(-1)), coumaric acid glucoside (35.68-49.22 g kg(-1)) and ferulic acid glucoside (5.07-15.23 g kg(-1)). The phytochemical profiles of co-extracts featured the major phenolic compounds from both barley and flaxseed hulls. The total phenolic content and 2,2-diphenyl-1-picrylhydrazyl radical-scavenging capacity varied significantly (P < 0.05) among different varieties of flaxseed and barley hulls. CONCLUSION: As agricultural by-products, barley and flaxseed hulls may be utilised as potential sources of functional food ingredients through extraction and concentration of the phytochemicals identified above. SN - 1097-0010 UR - https://www.unboundmedicine.com/medline/citation/22278383/Qualitative_and_quantitative_analysis_of_the_major_phenolic_compounds_as_antioxidants_in_barley_and_flaxseed_hulls_using_HPLC/MS/MS_ L2 - https://doi.org/10.1002/jsfa.5582 DB - PRIME DP - Unbound Medicine ER -