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Astragaloside IV prevents MPP⁺-induced SH-SY5Y cell death via the inhibition of Bax-mediated pathways and ROS production.
Mol Cell Biochem. 2012 May; 364(1-2):209-16.MC

Abstract

Parkinson's disease (PD) is characterized by a progressive degeneration of dopaminergic neurons in the substantia nigra. Oxidative stress and neural degeneration are suggested to be involved in the pathogenesis of PD. Previous studies have revealed that Astragaloside IV (AS-IV) can reduce inflammation and oxidation, making it a potential therapeutic agent for neurodegenerative disease. In this study, we investigated whether AS-IV protect against 1-methyl-4-phenylpyridnium ion (MPP(+))-induced dopaminergic neurotoxicity in SH-SY5Y cells and determined the mechanism of AS-IV neuroprotection. We found that pretreatment with AS-IV significantly reversed the loss of cell viability, nuclear condensation, the generation of intracellular reactive oxygen species (ROS), and the increase in Bax/Bcl-2 ratio and the activity of caspase-3 induced by MPP(+). Our study suggests that the neuroprotective effect of AS-IV is related to mechanisms including ROS production and the inhibition of Bax-mediated pathway. The present study supports the notion that AS-IV may be a promising neuroprotective agent for the treatment of neurodegenerative disorders such as PD.

Authors+Show Affiliations

Department of Neurosurgery and Institute for Functional Brain Disorders, Tangdu Hospital, The Fourth Military Medical University, 1 Xinsi Road, Baqiao District, Xi'an, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22278385

Citation

Zhang, Zhi-Guo, et al. "Astragaloside IV Prevents MPP⁺-induced SH-SY5Y Cell Death Via the Inhibition of Bax-mediated Pathways and ROS Production." Molecular and Cellular Biochemistry, vol. 364, no. 1-2, 2012, pp. 209-16.
Zhang ZG, Wu L, Wang JL, et al. Astragaloside IV prevents MPP⁺-induced SH-SY5Y cell death via the inhibition of Bax-mediated pathways and ROS production. Mol Cell Biochem. 2012;364(1-2):209-16.
Zhang, Z. G., Wu, L., Wang, J. L., Yang, J. D., Zhang, J., Zhang, J., Li, L. H., Xia, Y., Yao, L. B., Qin, H. Z., & Gao, G. D. (2012). Astragaloside IV prevents MPP⁺-induced SH-SY5Y cell death via the inhibition of Bax-mediated pathways and ROS production. Molecular and Cellular Biochemistry, 364(1-2), 209-16. https://doi.org/10.1007/s11010-011-1219-1
Zhang ZG, et al. Astragaloside IV Prevents MPP⁺-induced SH-SY5Y Cell Death Via the Inhibition of Bax-mediated Pathways and ROS Production. Mol Cell Biochem. 2012;364(1-2):209-16. PubMed PMID: 22278385.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Astragaloside IV prevents MPP⁺-induced SH-SY5Y cell death via the inhibition of Bax-mediated pathways and ROS production. AU - Zhang,Zhi-Guo, AU - Wu,Lin, AU - Wang,Ju-Lei, AU - Yang,Jian-Dong, AU - Zhang,Jing, AU - Zhang,Jian, AU - Li,Li-Hong, AU - Xia,Yi, AU - Yao,Li-Bo, AU - Qin,Huai-Zhou, AU - Gao,Guo-Dong, Y1 - 2012/01/26/ PY - 2011/08/29/received PY - 2011/12/21/accepted PY - 2012/1/27/entrez PY - 2012/1/27/pubmed PY - 2012/9/21/medline SP - 209 EP - 16 JF - Molecular and cellular biochemistry JO - Mol Cell Biochem VL - 364 IS - 1-2 N2 - Parkinson's disease (PD) is characterized by a progressive degeneration of dopaminergic neurons in the substantia nigra. Oxidative stress and neural degeneration are suggested to be involved in the pathogenesis of PD. Previous studies have revealed that Astragaloside IV (AS-IV) can reduce inflammation and oxidation, making it a potential therapeutic agent for neurodegenerative disease. In this study, we investigated whether AS-IV protect against 1-methyl-4-phenylpyridnium ion (MPP(+))-induced dopaminergic neurotoxicity in SH-SY5Y cells and determined the mechanism of AS-IV neuroprotection. We found that pretreatment with AS-IV significantly reversed the loss of cell viability, nuclear condensation, the generation of intracellular reactive oxygen species (ROS), and the increase in Bax/Bcl-2 ratio and the activity of caspase-3 induced by MPP(+). Our study suggests that the neuroprotective effect of AS-IV is related to mechanisms including ROS production and the inhibition of Bax-mediated pathway. The present study supports the notion that AS-IV may be a promising neuroprotective agent for the treatment of neurodegenerative disorders such as PD. SN - 1573-4919 UR - https://www.unboundmedicine.com/medline/citation/22278385/Astragaloside_IV_prevents_MPP⁺_induced_SH_SY5Y_cell_death_via_the_inhibition_of_Bax_mediated_pathways_and_ROS_production_ L2 - https://doi.org/10.1007/s11010-011-1219-1 DB - PRIME DP - Unbound Medicine ER -