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Synthesis and application of molecularly imprinted poly(methacrylic acid)-silica hybrid composite material for selective solid-phase extraction and high-performance liquid chromatography determination of oxytetracycline residues in milk.
J Chromatogr A. 2012 Mar 02; 1227:48-53.JC

Abstract

A novel molecularly imprinted organic-inorganic hybrid composite material (MIP-HCM) was developed based on molecular imprinting technique in combination with hybrid composite synthesis and sol-gel technology for selective solid-phase extraction (SPE) of tetracyclines residues in milk. The MIP-HCM was prepared using oxytetracycline as the template, methacrylic acid as organic functional monomer, tetraethoxysilane as inorganic precursor and methacryloxypropyltrimethoxysilane as the coupling agent. Synthesis conditions are optimized by changing some factors to obtain sorbent with the controllable adsorption capacity, selectivity, hardness and toughness. Binding study demonstrated that the imprinted hybrid composites showed excellent affinity and high selectivity to oxytetracycline. An enrichment factor of 18.8 along with a good sample clean-up was obtained under the optimized SPE conditions. The average recoveries of three tetracyclines antibiotics spiked milk at 0.1, 0.2 and 0.5 mg kg⁻¹ were in the range of 80.9-104.3% with the precision of 1.5-5.0%. The limits of detection and quantitation of the proposed method were in a range of 4.8-12.7 μg kg⁻¹ and 16.0-42.3 μg kg⁻¹, respectively.

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Authors+Show Affiliations

Lv YK
College of Chemistry and Environmental Science, Hebei University, Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding 071002, China. lvyunkai@hbu.edu.cn
Wang LM
No affiliation info available
Yang L
No affiliation info available
Zhao CX
No affiliation info available
Sun HW
No affiliation info available

MeSH

AnimalsChromatography, High Pressure LiquidDrug ResiduesLimit of DetectionLinear ModelsMilkMolecular ImprintingOxytetracyclinePolymethacrylic AcidsReproducibility of ResultsSolid Phase Extraction

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22281508

Citation

Lv, Yun-Kai, et al. "Synthesis and Application of Molecularly Imprinted Poly(methacrylic Acid)-silica Hybrid Composite Material for Selective Solid-phase Extraction and High-performance Liquid Chromatography Determination of Oxytetracycline Residues in Milk." Journal of Chromatography. A, vol. 1227, 2012, pp. 48-53.
Lv YK, Wang LM, Yang L, et al. Synthesis and application of molecularly imprinted poly(methacrylic acid)-silica hybrid composite material for selective solid-phase extraction and high-performance liquid chromatography determination of oxytetracycline residues in milk. J Chromatogr A. 2012;1227:48-53.
Lv, Y. K., Wang, L. M., Yang, L., Zhao, C. X., & Sun, H. W. (2012). Synthesis and application of molecularly imprinted poly(methacrylic acid)-silica hybrid composite material for selective solid-phase extraction and high-performance liquid chromatography determination of oxytetracycline residues in milk. Journal of Chromatography. A, 1227, 48-53. https://doi.org/10.1016/j.chroma.2011.12.108
Lv YK, et al. Synthesis and Application of Molecularly Imprinted Poly(methacrylic Acid)-silica Hybrid Composite Material for Selective Solid-phase Extraction and High-performance Liquid Chromatography Determination of Oxytetracycline Residues in Milk. J Chromatogr A. 2012 Mar 2;1227:48-53. PubMed PMID: 22281508.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Synthesis and application of molecularly imprinted poly(methacrylic acid)-silica hybrid composite material for selective solid-phase extraction and high-performance liquid chromatography determination of oxytetracycline residues in milk. AU - Lv,Yun-Kai, AU - Wang,Li-Min, AU - Yang,Lei, AU - Zhao,Chen-Xi, AU - Sun,Han-Wen, Y1 - 2012/01/11/ PY - 2011/10/24/received PY - 2011/12/23/revised PY - 2011/12/29/accepted PY - 2012/1/28/entrez PY - 2012/1/28/pubmed PY - 2012/4/20/medline SP - 48 EP - 53 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1227 N2 - A novel molecularly imprinted organic-inorganic hybrid composite material (MIP-HCM) was developed based on molecular imprinting technique in combination with hybrid composite synthesis and sol-gel technology for selective solid-phase extraction (SPE) of tetracyclines residues in milk. The MIP-HCM was prepared using oxytetracycline as the template, methacrylic acid as organic functional monomer, tetraethoxysilane as inorganic precursor and methacryloxypropyltrimethoxysilane as the coupling agent. Synthesis conditions are optimized by changing some factors to obtain sorbent with the controllable adsorption capacity, selectivity, hardness and toughness. Binding study demonstrated that the imprinted hybrid composites showed excellent affinity and high selectivity to oxytetracycline. An enrichment factor of 18.8 along with a good sample clean-up was obtained under the optimized SPE conditions. The average recoveries of three tetracyclines antibiotics spiked milk at 0.1, 0.2 and 0.5 mg kg⁻¹ were in the range of 80.9-104.3% with the precision of 1.5-5.0%. The limits of detection and quantitation of the proposed method were in a range of 4.8-12.7 μg kg⁻¹ and 16.0-42.3 μg kg⁻¹, respectively. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/22281508/Synthesis_and_application_of_molecularly_imprinted_poly_methacrylic_acid__silica_hybrid_composite_material_for_selective_solid_phase_extraction_and_high_performance_liquid_chromatography_determination_of_oxytetracycline_residues_in_milk_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(12)00070-2 DB - PRIME DP - Unbound Medicine ER -
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