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Effects of testosterone on norepinephrine release in isolated rat heart.
J Huazhong Univ Sci Technolog Med Sci. 2012 Feb; 32(1):42-46.JH

Abstract

The effects of testosterone on norepinephrine release were investigated in the isolated rat hearts. Sprague-Dawley male rats (n=120) were randomized to testosterone and control groups. The rats in testosterone group were perfused with modified Krebs-Henseleit buffer containing different concentrations of testosterone (0.1, 1.0, 10.0, and 100.0 nmol/L, respectively). Myocardial ischemia was induced by globally stopping the perfusion flow. Exocytotic norepinephrine release was induced by electrical field stimulation at 5 V (effective voltage) and 6 Hz (pulse width of 2 ms) for 1 min. The overflow of norepinephrine was determined by high pressure liquid chromatography and electrochemical detection (HPLC-EC). Following acute ischemia, testosterone (1.0, 10.0 and 100.0 nmol/L) significantly reduced norepinephrine release (P<0.01), and the norepinepherine overflow was similar between the control and 0.1 nmol/L testosterone group (P>0.05). Electrical stimulation of the ventricle evoked norepinepherine release, and this was diminished by the perfusion with testosterone at the concentrations of 1.0, 10.0 and 100.0 nmol/L (P<0.01). It is suggested that testosterone suppresses ischemia- and electrical stimulation-induced norepinepherine release in the isolated rat hearts.

Authors+Show Affiliations

Department of Cardiology, Ren Ji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200127, China.Department of Cardiology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450000, China.Department of Cardiology, Ren Ji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200127, China.Department of Cardiology, Ren Ji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200127, China.Department of Cardiology, Ren Ji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200127, China. heben@medmail.com.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22282243

Citation

Wang, Xiaofei, et al. "Effects of Testosterone On Norepinephrine Release in Isolated Rat Heart." Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban, vol. 32, no. 1, 2012, pp. 42-46.
Wang X, Zhang Y, Bu J, et al. Effects of testosterone on norepinephrine release in isolated rat heart. J Huazhong Univ Sci Technolog Med Sci. 2012;32(1):42-46.
Wang, X., Zhang, Y., Bu, J., Shen, L., & He, B. (2012). Effects of testosterone on norepinephrine release in isolated rat heart. Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban, 32(1), 42-46. https://doi.org/10.1007/s11596-012-0007-9
Wang X, et al. Effects of Testosterone On Norepinephrine Release in Isolated Rat Heart. J Huazhong Univ Sci Technolog Med Sci. 2012;32(1):42-46. PubMed PMID: 22282243.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of testosterone on norepinephrine release in isolated rat heart. AU - Wang,Xiaofei, AU - Zhang,Yanzhou, AU - Bu,Jun, AU - Shen,Linghong, AU - He,Ben, Y1 - 2012/01/27/ PY - 2010/11/26/received PY - 2012/1/28/entrez PY - 2012/1/28/pubmed PY - 2014/9/12/medline SP - 42 EP - 46 JF - Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban JO - J Huazhong Univ Sci Technolog Med Sci VL - 32 IS - 1 N2 - The effects of testosterone on norepinephrine release were investigated in the isolated rat hearts. Sprague-Dawley male rats (n=120) were randomized to testosterone and control groups. The rats in testosterone group were perfused with modified Krebs-Henseleit buffer containing different concentrations of testosterone (0.1, 1.0, 10.0, and 100.0 nmol/L, respectively). Myocardial ischemia was induced by globally stopping the perfusion flow. Exocytotic norepinephrine release was induced by electrical field stimulation at 5 V (effective voltage) and 6 Hz (pulse width of 2 ms) for 1 min. The overflow of norepinephrine was determined by high pressure liquid chromatography and electrochemical detection (HPLC-EC). Following acute ischemia, testosterone (1.0, 10.0 and 100.0 nmol/L) significantly reduced norepinephrine release (P<0.01), and the norepinepherine overflow was similar between the control and 0.1 nmol/L testosterone group (P>0.05). Electrical stimulation of the ventricle evoked norepinepherine release, and this was diminished by the perfusion with testosterone at the concentrations of 1.0, 10.0 and 100.0 nmol/L (P<0.01). It is suggested that testosterone suppresses ischemia- and electrical stimulation-induced norepinepherine release in the isolated rat hearts. SN - 1672-0733 UR - https://www.unboundmedicine.com/medline/citation/22282243/abstract/Effects_of_testosterone_on_norepinephrine_release_in_isolated_rat_heart_ L2 - https://doi.org/10.1007/s11596-012-0007-9 DB - PRIME DP - Unbound Medicine ER -