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Size-controlled and monodisperse enzyme-encapsulated chitosan microspheres developed by the SPG membrane emulsification technique.
J Colloid Interface Sci. 2012 Apr 01; 371(1):46-51.JC

Abstract

Lysozyme-encapsulated chitosan microspheres with micron-size diameters were successfully prepared for the first time by employing the Shirasu porous glass (SPG) membrane emulsification technique followed by cross-linking with glutaraldehyde, and the relationships between the preparation conditions and characteristics of the microspheres were studied in detail. This preparation method provided size-controllability and monodispersity of the microspheres, owing to the sharpness of the pore sizes of the SPG membranes. It was also possible to predict the average diameters of the enzyme-encapsulated microspheres using no fitting parameters, on the basis that each microsphere is prepared in an emulsion containing chitosan and lysozyme, without any collisions or aggregation occurring. X-ray photoelectron spectroscopy measurements indicated that the amount of encapsulated lysozyme was controlled by the concentrations of chitosan and lysozymes in the dispersion phase used for preparing the emulsions from which the enzyme-encapsulated microspheres are formed. Finally, the apparent activity of the encapsulated lysozymes was measured by the viscosimetric method, using ethyleneglycolchitin. Results showed that about half of the activity of the encapsulated lysozymes was maintained during the preparation procedure when employing the SPG membrane emulsification technique.

Authors+Show Affiliations

Department of Environmental and Energy Chemistry, Faculty of Engineering, Kogakuin University, Tokyo, Japan. akamatsu@cc.kogakuin.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22285100

Citation

Akamatsu, Kazuki, et al. "Size-controlled and Monodisperse Enzyme-encapsulated Chitosan Microspheres Developed By the SPG Membrane Emulsification Technique." Journal of Colloid and Interface Science, vol. 371, no. 1, 2012, pp. 46-51.
Akamatsu K, Ikeuchi Y, Nakao A, et al. Size-controlled and monodisperse enzyme-encapsulated chitosan microspheres developed by the SPG membrane emulsification technique. J Colloid Interface Sci. 2012;371(1):46-51.
Akamatsu, K., Ikeuchi, Y., Nakao, A., & Nakao, S. (2012). Size-controlled and monodisperse enzyme-encapsulated chitosan microspheres developed by the SPG membrane emulsification technique. Journal of Colloid and Interface Science, 371(1), 46-51. https://doi.org/10.1016/j.jcis.2011.12.078
Akamatsu K, et al. Size-controlled and Monodisperse Enzyme-encapsulated Chitosan Microspheres Developed By the SPG Membrane Emulsification Technique. J Colloid Interface Sci. 2012 Apr 1;371(1):46-51. PubMed PMID: 22285100.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Size-controlled and monodisperse enzyme-encapsulated chitosan microspheres developed by the SPG membrane emulsification technique. AU - Akamatsu,Kazuki, AU - Ikeuchi,Yuto, AU - Nakao,Aiko, AU - Nakao,Shin-ichi, Y1 - 2012/01/12/ PY - 2011/11/23/received PY - 2011/12/29/revised PY - 2011/12/30/accepted PY - 2012/1/31/entrez PY - 2012/1/31/pubmed PY - 2012/6/6/medline SP - 46 EP - 51 JF - Journal of colloid and interface science JO - J Colloid Interface Sci VL - 371 IS - 1 N2 - Lysozyme-encapsulated chitosan microspheres with micron-size diameters were successfully prepared for the first time by employing the Shirasu porous glass (SPG) membrane emulsification technique followed by cross-linking with glutaraldehyde, and the relationships between the preparation conditions and characteristics of the microspheres were studied in detail. This preparation method provided size-controllability and monodispersity of the microspheres, owing to the sharpness of the pore sizes of the SPG membranes. It was also possible to predict the average diameters of the enzyme-encapsulated microspheres using no fitting parameters, on the basis that each microsphere is prepared in an emulsion containing chitosan and lysozyme, without any collisions or aggregation occurring. X-ray photoelectron spectroscopy measurements indicated that the amount of encapsulated lysozyme was controlled by the concentrations of chitosan and lysozymes in the dispersion phase used for preparing the emulsions from which the enzyme-encapsulated microspheres are formed. Finally, the apparent activity of the encapsulated lysozymes was measured by the viscosimetric method, using ethyleneglycolchitin. Results showed that about half of the activity of the encapsulated lysozymes was maintained during the preparation procedure when employing the SPG membrane emulsification technique. SN - 1095-7103 UR - https://www.unboundmedicine.com/medline/citation/22285100/Size_controlled_and_monodisperse_enzyme_encapsulated_chitosan_microspheres_developed_by_the_SPG_membrane_emulsification_technique_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9797(12)00010-0 DB - PRIME DP - Unbound Medicine ER -