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Potentiality of standardized extract and isolated flavonoids from Zuccagnia punctata for the treatment of respiratory infections by Streptococcus pneumoniae: in vitro and in vivo studies.
J Ethnopharmacol. 2012 Mar 27; 140(2):287-92.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Zuccagnia punctata Cav. (Fabaceae) is a monotypic species distributed in western Argentina and is traditionally used for the treatment of bacterial and fungal infections. The aim of this study was to demonstrated the antibacterial activity of the Zuccagnia punctata standardized extract and the structurally related non-methoxylated flavonoids with similar pattern of substitution and differences in ring C present in this plant species: 7-hydroxyflavanone (HF), 2',4'-dihydroxychalcone (DHC) and 3,7-dihydroxyflavone (DHF), against Streptococcus pneumoniae clinical isolates using in vitro and in vivo models.

MATERIALS AND METHODS

MIC values of natural products were determined by agar macrodilution method. In vivo activities were investigated in a Streptococcus pneumoniae infection model in mice. Lung and blood samples were obtained for bacterial cell counts. The serum was used by biochemical analysis (alanine transaminase, aspartate transaminase, urea and creatinine) in order to evaluate the toxicity of natural products.

RESULTS

All samples showed antimicrobial activity in vitro with MIC values between 50 and 500μg/ml. Zuccagnia punctata extract (1mg/mice) and HF (1mg/mice) significantly reduced the number of viable Streptococcus pneumoniae in lung (p<0.01) while lower quantities has not effect. Therefore, the present study has shown that intake once or twice a day of 1mg of Zuccagnia punctata extract or HF for seven days did not result in toxicity.

CONCLUSIONS

Our results showed that Zuccagnia punctata extract as well as one of its isolated flavonoids, 7-hydroxyflavanone, could be useful for the development of a novel respiratory infections treatment.

Authors+Show Affiliations

Laboratorio de Bioquímica y Clínica Experimental, Centro de Referencia para Lactobacilos (CERELA-CONICET), Chacabuco 145, 4000 San Miguel de Tucumán, Tucumán, Argentina.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22285202

Citation

Zampini, I C., et al. "Potentiality of Standardized Extract and Isolated Flavonoids From Zuccagnia Punctata for the Treatment of Respiratory Infections By Streptococcus Pneumoniae: in Vitro and in Vivo Studies." Journal of Ethnopharmacology, vol. 140, no. 2, 2012, pp. 287-92.
Zampini IC, Villena J, Salva S, et al. Potentiality of standardized extract and isolated flavonoids from Zuccagnia punctata for the treatment of respiratory infections by Streptococcus pneumoniae: in vitro and in vivo studies. J Ethnopharmacol. 2012;140(2):287-92.
Zampini, I. C., Villena, J., Salva, S., Herrera, M., Isla, M. I., & Alvarez, S. (2012). Potentiality of standardized extract and isolated flavonoids from Zuccagnia punctata for the treatment of respiratory infections by Streptococcus pneumoniae: in vitro and in vivo studies. Journal of Ethnopharmacology, 140(2), 287-92. https://doi.org/10.1016/j.jep.2012.01.019
Zampini IC, et al. Potentiality of Standardized Extract and Isolated Flavonoids From Zuccagnia Punctata for the Treatment of Respiratory Infections By Streptococcus Pneumoniae: in Vitro and in Vivo Studies. J Ethnopharmacol. 2012 Mar 27;140(2):287-92. PubMed PMID: 22285202.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Potentiality of standardized extract and isolated flavonoids from Zuccagnia punctata for the treatment of respiratory infections by Streptococcus pneumoniae: in vitro and in vivo studies. AU - Zampini,I C, AU - Villena,J, AU - Salva,S, AU - Herrera,M, AU - Isla,M I, AU - Alvarez,S, Y1 - 2012/01/21/ PY - 2011/11/25/received PY - 2012/01/12/revised PY - 2012/01/13/accepted PY - 2012/1/31/entrez PY - 2012/1/31/pubmed PY - 2012/8/3/medline SP - 287 EP - 92 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 140 IS - 2 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Zuccagnia punctata Cav. (Fabaceae) is a monotypic species distributed in western Argentina and is traditionally used for the treatment of bacterial and fungal infections. The aim of this study was to demonstrated the antibacterial activity of the Zuccagnia punctata standardized extract and the structurally related non-methoxylated flavonoids with similar pattern of substitution and differences in ring C present in this plant species: 7-hydroxyflavanone (HF), 2',4'-dihydroxychalcone (DHC) and 3,7-dihydroxyflavone (DHF), against Streptococcus pneumoniae clinical isolates using in vitro and in vivo models. MATERIALS AND METHODS: MIC values of natural products were determined by agar macrodilution method. In vivo activities were investigated in a Streptococcus pneumoniae infection model in mice. Lung and blood samples were obtained for bacterial cell counts. The serum was used by biochemical analysis (alanine transaminase, aspartate transaminase, urea and creatinine) in order to evaluate the toxicity of natural products. RESULTS: All samples showed antimicrobial activity in vitro with MIC values between 50 and 500μg/ml. Zuccagnia punctata extract (1mg/mice) and HF (1mg/mice) significantly reduced the number of viable Streptococcus pneumoniae in lung (p<0.01) while lower quantities has not effect. Therefore, the present study has shown that intake once or twice a day of 1mg of Zuccagnia punctata extract or HF for seven days did not result in toxicity. CONCLUSIONS: Our results showed that Zuccagnia punctata extract as well as one of its isolated flavonoids, 7-hydroxyflavanone, could be useful for the development of a novel respiratory infections treatment. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/22285202/Potentiality_of_standardized_extract_and_isolated_flavonoids_from_Zuccagnia_punctata_for_the_treatment_of_respiratory_infections_by_Streptococcus_pneumoniae:_in_vitro_and_in_vivo_studies_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(12)00032-3 DB - PRIME DP - Unbound Medicine ER -