Tags

Type your tag names separated by a space and hit enter

Changes in protein and mRNA expression levels of claudin family after mucosal lesion by intestinal ischemia/reperfusion.
Int J Pharm. 2012 Apr 15; 426(1-2):82-89.IJ

Abstract

Ischemia/reperfusion (I/R) injury of the intestine is the leading cause of organ dysfunction after restoration of blood flow after diverse events, including shock and intestinal transplantation. I/R injury must be overcome for successful small intestinal transplantation. Tight junctions (TJ) are the most apical component of the intercellular junctional complex in epithelial cells; they establish cell polarity and functioning as major determinants of epithelial barrier function. Among the proteins that comprise TJ, the claudin family is thought to play a crucial role in homeostasis in multicellular organisms. Therefore, the aim of this study was to examine the changes in function of TJ and behavior of the claudin family during intestinal I/R. Wistar/ST rats underwent intestinal ischemia by using the spring scale and surgical suture for 1h, followed by 24h of reperfusion. We examined the changes in area under the blood concentration curve (AUC) after oral administration of FD-4, which is a paracellular marker, and claudin-1, -2, -4, and -7 mRNA and protein expression levels in ileum. The structure of ileal mucosa was partly damaged and its function was diminished by intestinal I/R until 3h after reperfusion, but were almost recovered 24h after reperfusion. However, a time difference was shown between the recoveries of mucosal structure and function. Furthermore, a difference in the expression among various kinds of claudin was found. It was suggested that claudin-4 and multi-PDZ domain protein, which is a scaffolding protein, regulate intestinal paracellular permeability during intestinal I/R. Moreover, the changes in the expression level of claudin-2 were unique.

Authors+Show Affiliations

Department of Drug Absorption and Pharmacokinetics, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan. Electronic address: takizawa@toyaku.ac.jp.Department of Drug Absorption and Pharmacokinetics, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.Department of Drug Absorption and Pharmacokinetics, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.Department of Drug Absorption and Pharmacokinetics, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.Department of Drug Absorption and Pharmacokinetics, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22285474

Citation

Takizawa, Yusuke, et al. "Changes in Protein and mRNA Expression Levels of Claudin Family After Mucosal Lesion By Intestinal Ischemia/reperfusion." International Journal of Pharmaceutics, vol. 426, no. 1-2, 2012, pp. 82-89.
Takizawa Y, Kishimoto H, Kitazato T, et al. Changes in protein and mRNA expression levels of claudin family after mucosal lesion by intestinal ischemia/reperfusion. Int J Pharm. 2012;426(1-2):82-89.
Takizawa, Y., Kishimoto, H., Kitazato, T., Tomita, M., & Hayashi, M. (2012). Changes in protein and mRNA expression levels of claudin family after mucosal lesion by intestinal ischemia/reperfusion. International Journal of Pharmaceutics, 426(1-2), 82-89. https://doi.org/10.1016/j.ijpharm.2012.01.023
Takizawa Y, et al. Changes in Protein and mRNA Expression Levels of Claudin Family After Mucosal Lesion By Intestinal Ischemia/reperfusion. Int J Pharm. 2012 Apr 15;426(1-2):82-89. PubMed PMID: 22285474.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Changes in protein and mRNA expression levels of claudin family after mucosal lesion by intestinal ischemia/reperfusion. AU - Takizawa,Yusuke, AU - Kishimoto,Hisanao, AU - Kitazato,Takuya, AU - Tomita,Mikio, AU - Hayashi,Masahiro, Y1 - 2012/01/20/ PY - 2011/10/04/received PY - 2011/12/19/revised PY - 2012/01/12/accepted PY - 2012/1/31/entrez PY - 2012/1/31/pubmed PY - 2012/6/23/medline SP - 82 EP - 89 JF - International journal of pharmaceutics JO - Int J Pharm VL - 426 IS - 1-2 N2 - Ischemia/reperfusion (I/R) injury of the intestine is the leading cause of organ dysfunction after restoration of blood flow after diverse events, including shock and intestinal transplantation. I/R injury must be overcome for successful small intestinal transplantation. Tight junctions (TJ) are the most apical component of the intercellular junctional complex in epithelial cells; they establish cell polarity and functioning as major determinants of epithelial barrier function. Among the proteins that comprise TJ, the claudin family is thought to play a crucial role in homeostasis in multicellular organisms. Therefore, the aim of this study was to examine the changes in function of TJ and behavior of the claudin family during intestinal I/R. Wistar/ST rats underwent intestinal ischemia by using the spring scale and surgical suture for 1h, followed by 24h of reperfusion. We examined the changes in area under the blood concentration curve (AUC) after oral administration of FD-4, which is a paracellular marker, and claudin-1, -2, -4, and -7 mRNA and protein expression levels in ileum. The structure of ileal mucosa was partly damaged and its function was diminished by intestinal I/R until 3h after reperfusion, but were almost recovered 24h after reperfusion. However, a time difference was shown between the recoveries of mucosal structure and function. Furthermore, a difference in the expression among various kinds of claudin was found. It was suggested that claudin-4 and multi-PDZ domain protein, which is a scaffolding protein, regulate intestinal paracellular permeability during intestinal I/R. Moreover, the changes in the expression level of claudin-2 were unique. SN - 1873-3476 UR - https://www.unboundmedicine.com/medline/citation/22285474/Changes_in_protein_and_mRNA_expression_levels_of_claudin_family_after_mucosal_lesion_by_intestinal_ischemia/reperfusion_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-5173(12)00057-9 DB - PRIME DP - Unbound Medicine ER -