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Inhibition of lamina outgrowth following Solanum lycopersicum AUXIN RESPONSE FACTOR 10 (SlARF10) derepression.
Plant Mol Biol. 2012 Apr; 78(6):561-76.PM

Abstract

Auxin response factors (ARFs) are plant transcription factors that activate or repress the expression of auxin-responsive genes and accordingly, play key roles in auxin-mediated developmental processes. Here we identified and characterized the Solanum lycopersicum (tomato) ARF10 homolog (SlARF10), demonstrated that it is posttranscriptionally regulated by Sl-miR160, and investigated the significance of this regulation for tomato development. In wild-type tomato, SlARF10 is primarily expressed in the pericarp of mature and ripened fruit, showing an expression profile complementary to that of Sl-miR160. Constitutive expression of wild-type SlARF10 did not alter tomato development. However, transgenic tomato plants that constitutively expressed the Sl-miR160a-resistant version (mSlARF10) developed narrow leaflet blades, sepals and petals, and abnormally shaped fruit. During compound leaf development, mSlARF10 accumulation specifically inhibited leaflet blade outgrowth without affecting other auxin-driven processes such as leaflet initiation and lobe formation. Moreover, blade size was inversely correlated with mSlARF10 transcript levels, strongly implying that the SlARF10 protein, which was localized to the nucleus, can function as a transcriptional repressor of leaflet lamina outgrowth. Accordingly, known auxin-responsive genes, which promote cell growth, were downregulated in shoot apices that accumulated increased mSlARF10 levels. Taken together, we propose that repression of SlARF10 by Sl-miR160 is essential for auxin-mediated blade outgrowth and early fruit development.

Authors+Show Affiliations

Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22287097

Citation

Hendelman, A, et al. "Inhibition of Lamina Outgrowth Following Solanum Lycopersicum AUXIN RESPONSE FACTOR 10 (SlARF10) Derepression." Plant Molecular Biology, vol. 78, no. 6, 2012, pp. 561-76.
Hendelman A, Buxdorf K, Stav R, et al. Inhibition of lamina outgrowth following Solanum lycopersicum AUXIN RESPONSE FACTOR 10 (SlARF10) derepression. Plant Mol Biol. 2012;78(6):561-76.
Hendelman, A., Buxdorf, K., Stav, R., Kravchik, M., & Arazi, T. (2012). Inhibition of lamina outgrowth following Solanum lycopersicum AUXIN RESPONSE FACTOR 10 (SlARF10) derepression. Plant Molecular Biology, 78(6), 561-76. https://doi.org/10.1007/s11103-012-9883-4
Hendelman A, et al. Inhibition of Lamina Outgrowth Following Solanum Lycopersicum AUXIN RESPONSE FACTOR 10 (SlARF10) Derepression. Plant Mol Biol. 2012;78(6):561-76. PubMed PMID: 22287097.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition of lamina outgrowth following Solanum lycopersicum AUXIN RESPONSE FACTOR 10 (SlARF10) derepression. AU - Hendelman,A, AU - Buxdorf,K, AU - Stav,R, AU - Kravchik,M, AU - Arazi,T, PY - 2011/06/12/received PY - 2012/01/12/accepted PY - 2012/1/31/entrez PY - 2012/1/31/pubmed PY - 2012/5/19/medline SP - 561 EP - 76 JF - Plant molecular biology JO - Plant Mol Biol VL - 78 IS - 6 N2 - Auxin response factors (ARFs) are plant transcription factors that activate or repress the expression of auxin-responsive genes and accordingly, play key roles in auxin-mediated developmental processes. Here we identified and characterized the Solanum lycopersicum (tomato) ARF10 homolog (SlARF10), demonstrated that it is posttranscriptionally regulated by Sl-miR160, and investigated the significance of this regulation for tomato development. In wild-type tomato, SlARF10 is primarily expressed in the pericarp of mature and ripened fruit, showing an expression profile complementary to that of Sl-miR160. Constitutive expression of wild-type SlARF10 did not alter tomato development. However, transgenic tomato plants that constitutively expressed the Sl-miR160a-resistant version (mSlARF10) developed narrow leaflet blades, sepals and petals, and abnormally shaped fruit. During compound leaf development, mSlARF10 accumulation specifically inhibited leaflet blade outgrowth without affecting other auxin-driven processes such as leaflet initiation and lobe formation. Moreover, blade size was inversely correlated with mSlARF10 transcript levels, strongly implying that the SlARF10 protein, which was localized to the nucleus, can function as a transcriptional repressor of leaflet lamina outgrowth. Accordingly, known auxin-responsive genes, which promote cell growth, were downregulated in shoot apices that accumulated increased mSlARF10 levels. Taken together, we propose that repression of SlARF10 by Sl-miR160 is essential for auxin-mediated blade outgrowth and early fruit development. SN - 1573-5028 UR - https://www.unboundmedicine.com/medline/citation/22287097/Inhibition_of_lamina_outgrowth_following_Solanum_lycopersicum_AUXIN_RESPONSE_FACTOR_10__SlARF10__derepression_ L2 - https://doi.org/10.1007/s11103-012-9883-4 DB - PRIME DP - Unbound Medicine ER -