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GDNF-treated acellular nerve graft promotes motoneuron axon regeneration after implantation into cervical root avulsed spinal cord.
Neuropathol Appl Neurobiol. 2012 Dec; 38(7):681-95.NA

Abstract

It is well known that glial cell line-derived neurotrophic factor (GDNF) is a potent neurotrophic factor for motoneurons. We have previously shown that it greatly enhanced motoneuron survival and axon regeneration after implantation of peripheral nerve graft following spinal root avulsion.

AIMS

In the current study, we explore whether injection of GDNF promotes axon regeneration in decellularized nerve induced by repeated freeze-thaw cycles.

METHODS

We injected saline or GDNF into the decellularized nerve after root avulsion in adult Sprague-Dawley rats and assessed motoneuron axon regeneration and Schwann cell migration by retrograde labelling and immunohistochemistry.

RESULTS

We found that no axons were present in saline-treated acellular nerve whereas Schwann cells migrated into GDNF-treated acellular nerve grafts. We also found that Schwann cells migrated into the nerve grafts as early as 4 days after implantation, coinciding with the first appearance of regenerating axons in the grafts. Application of GDNF outside the graft did not induce Schwann cell infiltration nor axon regeneration into the graft. Application of pleiotrophin, a trophic factor which promotes axon regeneration but not Schwann cell migration, did not promote axon infiltration into acellular nerve graft.

CONCLUSIONS

We conclude that GDNF induced Schwann cell migration and axon regeneration into the acellular nerve graft. Our findings can be of potential clinical value to develop acellular nerve grafting for use in spinal root avulsion injuries.

Authors+Show Affiliations

Department of Anatomy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22289090

Citation

Chu, T-H, et al. "GDNF-treated Acellular Nerve Graft Promotes Motoneuron Axon Regeneration After Implantation Into Cervical Root Avulsed Spinal Cord." Neuropathology and Applied Neurobiology, vol. 38, no. 7, 2012, pp. 681-95.
Chu TH, Wang L, Guo A, et al. GDNF-treated acellular nerve graft promotes motoneuron axon regeneration after implantation into cervical root avulsed spinal cord. Neuropathol Appl Neurobiol. 2012;38(7):681-95.
Chu, T. H., Wang, L., Guo, A., Chan, V. W., Wong, C. W., & Wu, W. (2012). GDNF-treated acellular nerve graft promotes motoneuron axon regeneration after implantation into cervical root avulsed spinal cord. Neuropathology and Applied Neurobiology, 38(7), 681-95. https://doi.org/10.1111/j.1365-2990.2012.01253.x
Chu TH, et al. GDNF-treated Acellular Nerve Graft Promotes Motoneuron Axon Regeneration After Implantation Into Cervical Root Avulsed Spinal Cord. Neuropathol Appl Neurobiol. 2012;38(7):681-95. PubMed PMID: 22289090.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - GDNF-treated acellular nerve graft promotes motoneuron axon regeneration after implantation into cervical root avulsed spinal cord. AU - Chu,T-H, AU - Wang,L, AU - Guo,A, AU - Chan,V W-K, AU - Wong,C W-M, AU - Wu,W, PY - 2012/2/1/entrez PY - 2012/2/1/pubmed PY - 2013/4/25/medline SP - 681 EP - 95 JF - Neuropathology and applied neurobiology JO - Neuropathol Appl Neurobiol VL - 38 IS - 7 N2 - UNLABELLED: It is well known that glial cell line-derived neurotrophic factor (GDNF) is a potent neurotrophic factor for motoneurons. We have previously shown that it greatly enhanced motoneuron survival and axon regeneration after implantation of peripheral nerve graft following spinal root avulsion. AIMS: In the current study, we explore whether injection of GDNF promotes axon regeneration in decellularized nerve induced by repeated freeze-thaw cycles. METHODS: We injected saline or GDNF into the decellularized nerve after root avulsion in adult Sprague-Dawley rats and assessed motoneuron axon regeneration and Schwann cell migration by retrograde labelling and immunohistochemistry. RESULTS: We found that no axons were present in saline-treated acellular nerve whereas Schwann cells migrated into GDNF-treated acellular nerve grafts. We also found that Schwann cells migrated into the nerve grafts as early as 4 days after implantation, coinciding with the first appearance of regenerating axons in the grafts. Application of GDNF outside the graft did not induce Schwann cell infiltration nor axon regeneration into the graft. Application of pleiotrophin, a trophic factor which promotes axon regeneration but not Schwann cell migration, did not promote axon infiltration into acellular nerve graft. CONCLUSIONS: We conclude that GDNF induced Schwann cell migration and axon regeneration into the acellular nerve graft. Our findings can be of potential clinical value to develop acellular nerve grafting for use in spinal root avulsion injuries. SN - 1365-2990 UR - https://www.unboundmedicine.com/medline/citation/22289090/GDNF_treated_acellular_nerve_graft_promotes_motoneuron_axon_regeneration_after_implantation_into_cervical_root_avulsed_spinal_cord_ L2 - https://doi.org/10.1111/j.1365-2990.2012.01253.x DB - PRIME DP - Unbound Medicine ER -