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Characterization of secondary volatile profiles in Nigella sativa seeds from two different origins using accelerated solvent extraction and gas chromatography-mass spectrometry.
Biomed Chromatogr. 2012 Oct; 26(10):1157-62.BC

Abstract

The extraction and identification of bioactive compounds from herbs is of great interest. In this study, accelerated solvent extraction (ASE) technique was used to analyze the secondary volatile profiles in Nigella sativa seeds obtained from two different origins, Egypt and Bangladesh. The main extraction parameters, including extraction temperature, pressure and static extraction time, were investigated and optimized. Identification and quantification of the major constituents in nonpolar extracts (hexane) were achieved by means of GC-FID/GC-MS analysis with external standards. The two seeds showed a similar variety of chemical composition; however, the secondary volatiles profile of Bangladesh seed was higher than that of the Egyptian seed. A total of 25 compounds were identified from the ASE extract under the following optimum extraction conditions: 100°C, 1500 psi and 5 min, for extraction temperature, pressure and static time, respectively. The proposed technique can be used for the characterization of N. sativa varieties or cultivars.

Authors+Show Affiliations

Natural Products Chemistry Laboratory, College of Agriculture and Life Science, Chonnam National University, 300 Yong-Bong Dong, Buk-Ku, Gwangju, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22290690

Citation

Liu, Xue, et al. "Characterization of Secondary Volatile Profiles in Nigella Sativa Seeds From Two Different Origins Using Accelerated Solvent Extraction and Gas Chromatography-mass Spectrometry." Biomedical Chromatography : BMC, vol. 26, no. 10, 2012, pp. 1157-62.
Liu X, Abd El-Aty AM, Cho SK, et al. Characterization of secondary volatile profiles in Nigella sativa seeds from two different origins using accelerated solvent extraction and gas chromatography-mass spectrometry. Biomed Chromatogr. 2012;26(10):1157-62.
Liu, X., Abd El-Aty, A. M., Cho, S. K., Yang, A., Park, J. H., & Shim, J. H. (2012). Characterization of secondary volatile profiles in Nigella sativa seeds from two different origins using accelerated solvent extraction and gas chromatography-mass spectrometry. Biomedical Chromatography : BMC, 26(10), 1157-62. https://doi.org/10.1002/bmc.2671
Liu X, et al. Characterization of Secondary Volatile Profiles in Nigella Sativa Seeds From Two Different Origins Using Accelerated Solvent Extraction and Gas Chromatography-mass Spectrometry. Biomed Chromatogr. 2012;26(10):1157-62. PubMed PMID: 22290690.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of secondary volatile profiles in Nigella sativa seeds from two different origins using accelerated solvent extraction and gas chromatography-mass spectrometry. AU - Liu,Xue, AU - Abd El-Aty,A M, AU - Cho,S-K, AU - Yang,Angel, AU - Park,Jong-Hyouk, AU - Shim,Jae-Han, Y1 - 2012/01/30/ PY - 2011/10/21/received PY - 2011/11/08/revised PY - 2011/11/08/accepted PY - 2012/2/1/entrez PY - 2012/2/1/pubmed PY - 2012/12/18/medline SP - 1157 EP - 62 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 26 IS - 10 N2 - The extraction and identification of bioactive compounds from herbs is of great interest. In this study, accelerated solvent extraction (ASE) technique was used to analyze the secondary volatile profiles in Nigella sativa seeds obtained from two different origins, Egypt and Bangladesh. The main extraction parameters, including extraction temperature, pressure and static extraction time, were investigated and optimized. Identification and quantification of the major constituents in nonpolar extracts (hexane) were achieved by means of GC-FID/GC-MS analysis with external standards. The two seeds showed a similar variety of chemical composition; however, the secondary volatiles profile of Bangladesh seed was higher than that of the Egyptian seed. A total of 25 compounds were identified from the ASE extract under the following optimum extraction conditions: 100°C, 1500 psi and 5 min, for extraction temperature, pressure and static time, respectively. The proposed technique can be used for the characterization of N. sativa varieties or cultivars. SN - 1099-0801 UR - https://www.unboundmedicine.com/medline/citation/22290690/Characterization_of_secondary_volatile_profiles_in_Nigella_sativa_seeds_from_two_different_origins_using_accelerated_solvent_extraction_and_gas_chromatography_mass_spectrometry_ L2 - https://doi.org/10.1002/bmc.2671 DB - PRIME DP - Unbound Medicine ER -