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Efficacy evaluation of live Escherichia coli expression Brucella P39 protein combined with CpG oligodeoxynucleotides vaccine against Brucella melitensis 16M, in BALB/c mice.
Biologicals 2012; 40(2):140-5B

Abstract

Brucella is gram-negative bacteria responsible for brucellosis in a wide variety of animals and humans. BALB/c mice were immunized with live Escherichia coli expression the p39 gene of Brucella melitensis, a gene coding for the periplasmic binding protein. Mice were injected with either E. coli BL21 (DE3) pEt15b or E. coli BL21 (DE3) pEt15b-p39 alone or adjuvanted with either CpG oligodeoxynucleotides (CpG ODN) or non-CpG ODN. E. coli BL21 (DE3) pEt15b-p39 with CpG ODN or with non-CpG ODN mice groups showed a significant IFN-γ production and T-cell proliferation as a reaction to P39 antigen. In addition, antibody responses (IgG, IgG1 and IgG2a), were only found in these two mice groups. A higher level of protection against B. melitensis 16M were observed in mice immunized with E. coli BL21 (DE3) pEt15b-p39 and CpG ODN comparing with those immunized with E. coli BL21 (DE3) pEt15b-p39 alone or with non-CpG ODN. No protection against B. melitensis 16M was observed in mice immunized with E. coli BL21 (DE3) pEt15b alone or with the adjuvant. Rev.1 protection at 4 and 8 weeks post-challenge was more effective than that observed with E. coli BL21 (DE3) pEt15b-p39 and CpG ODN.

Authors+Show Affiliations

Department of Molecular Biology and Biotechnology, Atomic Energy Commission, P.O. Box 6091, Damascus, Syria. ascientific@aec.org.syNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Studies
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22296786

Citation

Al-Mariri, Ayman, et al. "Efficacy Evaluation of Live Escherichia Coli Expression Brucella P39 Protein Combined With CpG Oligodeoxynucleotides Vaccine Against Brucella Melitensis 16M, in BALB/c Mice." Biologicals : Journal of the International Association of Biological Standardization, vol. 40, no. 2, 2012, pp. 140-5.
Al-Mariri A, Mahmoud NH, Hammoud R. Efficacy evaluation of live Escherichia coli expression Brucella P39 protein combined with CpG oligodeoxynucleotides vaccine against Brucella melitensis 16M, in BALB/c mice. Biologicals. 2012;40(2):140-5.
Al-Mariri, A., Mahmoud, N. H., & Hammoud, R. (2012). Efficacy evaluation of live Escherichia coli expression Brucella P39 protein combined with CpG oligodeoxynucleotides vaccine against Brucella melitensis 16M, in BALB/c mice. Biologicals : Journal of the International Association of Biological Standardization, 40(2), pp. 140-5. doi:10.1016/j.biologicals.2012.01.002.
Al-Mariri A, Mahmoud NH, Hammoud R. Efficacy Evaluation of Live Escherichia Coli Expression Brucella P39 Protein Combined With CpG Oligodeoxynucleotides Vaccine Against Brucella Melitensis 16M, in BALB/c Mice. Biologicals. 2012;40(2):140-5. PubMed PMID: 22296786.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Efficacy evaluation of live Escherichia coli expression Brucella P39 protein combined with CpG oligodeoxynucleotides vaccine against Brucella melitensis 16M, in BALB/c mice. AU - Al-Mariri,Ayman, AU - Mahmoud,Nermeen Haj, AU - Hammoud,Razan, Y1 - 2012/01/30/ PY - 2011/10/31/received PY - 2011/12/27/revised PY - 2012/01/03/accepted PY - 2012/2/3/entrez PY - 2012/2/3/pubmed PY - 2012/7/11/medline SP - 140 EP - 5 JF - Biologicals : journal of the International Association of Biological Standardization JO - Biologicals VL - 40 IS - 2 N2 - Brucella is gram-negative bacteria responsible for brucellosis in a wide variety of animals and humans. BALB/c mice were immunized with live Escherichia coli expression the p39 gene of Brucella melitensis, a gene coding for the periplasmic binding protein. Mice were injected with either E. coli BL21 (DE3) pEt15b or E. coli BL21 (DE3) pEt15b-p39 alone or adjuvanted with either CpG oligodeoxynucleotides (CpG ODN) or non-CpG ODN. E. coli BL21 (DE3) pEt15b-p39 with CpG ODN or with non-CpG ODN mice groups showed a significant IFN-γ production and T-cell proliferation as a reaction to P39 antigen. In addition, antibody responses (IgG, IgG1 and IgG2a), were only found in these two mice groups. A higher level of protection against B. melitensis 16M were observed in mice immunized with E. coli BL21 (DE3) pEt15b-p39 and CpG ODN comparing with those immunized with E. coli BL21 (DE3) pEt15b-p39 alone or with non-CpG ODN. No protection against B. melitensis 16M was observed in mice immunized with E. coli BL21 (DE3) pEt15b alone or with the adjuvant. Rev.1 protection at 4 and 8 weeks post-challenge was more effective than that observed with E. coli BL21 (DE3) pEt15b-p39 and CpG ODN. SN - 1095-8320 UR - https://www.unboundmedicine.com/medline/citation/22296786/Efficacy_evaluation_of_live_Escherichia_coli_expression_Brucella_P39_protein_combined_with_CpG_oligodeoxynucleotides_vaccine_against_Brucella_melitensis_16M_in_BALB/c_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1045-1056(12)00003-6 DB - PRIME DP - Unbound Medicine ER -