Tags

Type your tag names separated by a space and hit enter

E2F1, ARID3A/Bright and Oct-2 factors bind to the Epstein-Barr virus C promoter, EBNA1 and oriP, participating in long-distance promoter-enhancer interactions.
J Gen Virol. 2012 May; 93(Pt 5):1065-1075.JG

Abstract

The Epstein-Barr virus (EBV) C promoter (Cp) regulates several genes required for B-cell proliferation in latent EBV infection. The family of repeats (FR) region of the latent origin of plasmid replication (oriP) functions as an Epstein-Barr nuclear antigen 1 (EBNA1)-dependent distant enhancer of Cp activity, and the enhancer-promoter interaction is mediated by a higher-order multi-protein complex containing several copies of EBNA1. Using DNA-affinity purification with a 170 bp region of the Cp in combination with mass spectrometry, we identified the cell cycle-regulatory protein E2F1, the E2F-binding protein ARID3A, and the B-cell-specific transcription factor Oct-2 as components of this multi-protein complex. Binding of the three factors to the FR region of oriP was determined by DNA-affinity and immunoblot analysis. Co-immunoprecipitation and proximity ligation analysis revealed that the three factors, E2F1, ARID3A and Oct-2, interact with each other as well as with EBNA1 in the nuclei of EBV-positive cells. Using the chromatin immunoprecipitation assay, we showed that E2F1 and Oct-2 interacted with the FR part of oriP and the Cp, but the ARID3A interaction was, however, only detected at the Cp. Our findings support the hypothesis that EBNA1 initiates transcription at the Cp via interactions between multiple EBNA1 homodimers and cellular transcription factors in a large molecular machinery that forms a dynamic interaction between Cp and FR.

Authors+Show Affiliations

Department of Clinical Chemistry and Transfusion Medicine, Institute of Biomedicine, Sahlgrenska University Hospital, University of Gothenburg, SE-41345 Gothenburg, Sweden.Department of Clinical Chemistry and Transfusion Medicine, Institute of Biomedicine, Sahlgrenska University Hospital, University of Gothenburg, SE-41345 Gothenburg, Sweden.Department of Clinical Chemistry and Transfusion Medicine, Institute of Biomedicine, Sahlgrenska University Hospital, University of Gothenburg, SE-41345 Gothenburg, Sweden.Department of Clinical Chemistry and Transfusion Medicine, Institute of Biomedicine, Sahlgrenska University Hospital, University of Gothenburg, SE-41345 Gothenburg, Sweden.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22302879

Citation

Boreström, Cecilia, et al. "E2F1, ARID3A/Bright and Oct-2 Factors Bind to the Epstein-Barr Virus C Promoter, EBNA1 and oriP, Participating in Long-distance Promoter-enhancer Interactions." The Journal of General Virology, vol. 93, no. Pt 5, 2012, pp. 1065-1075.
Boreström C, Forsman A, Rüetschi U, et al. E2F1, ARID3A/Bright and Oct-2 factors bind to the Epstein-Barr virus C promoter, EBNA1 and oriP, participating in long-distance promoter-enhancer interactions. J Gen Virol. 2012;93(Pt 5):1065-1075.
Boreström, C., Forsman, A., Rüetschi, U., & Rymo, L. (2012). E2F1, ARID3A/Bright and Oct-2 factors bind to the Epstein-Barr virus C promoter, EBNA1 and oriP, participating in long-distance promoter-enhancer interactions. The Journal of General Virology, 93(Pt 5), 1065-1075. https://doi.org/10.1099/vir.0.038752-0
Boreström C, et al. E2F1, ARID3A/Bright and Oct-2 Factors Bind to the Epstein-Barr Virus C Promoter, EBNA1 and oriP, Participating in Long-distance Promoter-enhancer Interactions. J Gen Virol. 2012;93(Pt 5):1065-1075. PubMed PMID: 22302879.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - E2F1, ARID3A/Bright and Oct-2 factors bind to the Epstein-Barr virus C promoter, EBNA1 and oriP, participating in long-distance promoter-enhancer interactions. AU - Boreström,Cecilia, AU - Forsman,Alma, AU - Rüetschi,Ulla, AU - Rymo,Lars, Y1 - 2012/02/01/ PY - 2012/2/4/entrez PY - 2012/2/4/pubmed PY - 2012/6/7/medline SP - 1065 EP - 1075 JF - The Journal of general virology JO - J Gen Virol VL - 93 IS - Pt 5 N2 - The Epstein-Barr virus (EBV) C promoter (Cp) regulates several genes required for B-cell proliferation in latent EBV infection. The family of repeats (FR) region of the latent origin of plasmid replication (oriP) functions as an Epstein-Barr nuclear antigen 1 (EBNA1)-dependent distant enhancer of Cp activity, and the enhancer-promoter interaction is mediated by a higher-order multi-protein complex containing several copies of EBNA1. Using DNA-affinity purification with a 170 bp region of the Cp in combination with mass spectrometry, we identified the cell cycle-regulatory protein E2F1, the E2F-binding protein ARID3A, and the B-cell-specific transcription factor Oct-2 as components of this multi-protein complex. Binding of the three factors to the FR region of oriP was determined by DNA-affinity and immunoblot analysis. Co-immunoprecipitation and proximity ligation analysis revealed that the three factors, E2F1, ARID3A and Oct-2, interact with each other as well as with EBNA1 in the nuclei of EBV-positive cells. Using the chromatin immunoprecipitation assay, we showed that E2F1 and Oct-2 interacted with the FR part of oriP and the Cp, but the ARID3A interaction was, however, only detected at the Cp. Our findings support the hypothesis that EBNA1 initiates transcription at the Cp via interactions between multiple EBNA1 homodimers and cellular transcription factors in a large molecular machinery that forms a dynamic interaction between Cp and FR. SN - 1465-2099 UR - https://www.unboundmedicine.com/medline/citation/22302879/E2F1_ARID3A/Bright_and_Oct_2_factors_bind_to_the_Epstein_Barr_virus_C_promoter_EBNA1_and_oriP_participating_in_long_distance_promoter_enhancer_interactions_ L2 - http://jgv.microbiologyresearch.org/pubmed/content/journal/jgv/10.1099/vir.0.038752-0 DB - PRIME DP - Unbound Medicine ER -