Tags

Type your tag names separated by a space and hit enter

Iron overload-induced rat liver injury: Involvement of protein tyrosine nitration and the effect of baicalin.
Eur J Pharmacol. 2012 Apr 05; 680(1-3):95-101.EJ

Abstract

Baicalin has been reported to protect against liver injury in iron-overload mice, however, the mechanisms underlying the hepatoprotective properties of baicalin are poorly understood. In this study, we systematically studied the protective effect of baicalin on iron overload induced liver injury, as well as the underlying mechanism based on nitrative stress in rat model. We found that when iron overload rats (500mgiron/kg) were fed baicalin-containing diet (0.3% and 1% w/w) for 45days, baicalin dose dependently protected against iron overload induced liver injury, including alleviation of hepatic pathological damage, decrease of SOD activity, iron content, carbonyl content, and the thiobarbituric acid-reactive substances level in hepatic tissues. It also increased serum iron content, SH content and GPx activity, decreased serum ALT and AST activities. Immunohistochemistry and immunoprecipitation analysis revealed that baicalin could also inhibit iron overload induced protein tyrosine nitration in liver. Moreover, in iron overload rat liver, we found that baicalin decreased the iron overload increased level of glutathione-S-transferases (GSTs) expression, oxidation and nitration. These results suggest that not only oxidative stress, but also nitrative stress, is involved in iron overload induced liver injury, and the underlying mechanism might partially relate to the involvement of GSTs expression and post-translational modification. Baicalin can effectively prevent iron overload caused abnormality and can be a candidate medicine for iron overload diseases.

Authors+Show Affiliations

School of Chemistry and Chemical Engineering, Huazhong University of Science & Technology, Wuhan, 430074, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22306240

Citation

Zhang, Yan, et al. "Iron Overload-induced Rat Liver Injury: Involvement of Protein Tyrosine Nitration and the Effect of Baicalin." European Journal of Pharmacology, vol. 680, no. 1-3, 2012, pp. 95-101.
Zhang Y, Huang Y, Deng X, et al. Iron overload-induced rat liver injury: Involvement of protein tyrosine nitration and the effect of baicalin. Eur J Pharmacol. 2012;680(1-3):95-101.
Zhang, Y., Huang, Y., Deng, X., Xu, Y., Gao, Z., & Li, H. (2012). Iron overload-induced rat liver injury: Involvement of protein tyrosine nitration and the effect of baicalin. European Journal of Pharmacology, 680(1-3), 95-101. https://doi.org/10.1016/j.ejphar.2012.01.010
Zhang Y, et al. Iron Overload-induced Rat Liver Injury: Involvement of Protein Tyrosine Nitration and the Effect of Baicalin. Eur J Pharmacol. 2012 Apr 5;680(1-3):95-101. PubMed PMID: 22306240.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Iron overload-induced rat liver injury: Involvement of protein tyrosine nitration and the effect of baicalin. AU - Zhang,Yan, AU - Huang,Yi, AU - Deng,Xiaorong, AU - Xu,Yan, AU - Gao,Zhonghong, AU - Li,Hailing, Y1 - 2012/01/28/ PY - 2011/08/25/received PY - 2012/01/03/revised PY - 2012/01/13/accepted PY - 2012/2/7/entrez PY - 2012/2/7/pubmed PY - 2012/9/14/medline SP - 95 EP - 101 JF - European journal of pharmacology JO - Eur. J. Pharmacol. VL - 680 IS - 1-3 N2 - Baicalin has been reported to protect against liver injury in iron-overload mice, however, the mechanisms underlying the hepatoprotective properties of baicalin are poorly understood. In this study, we systematically studied the protective effect of baicalin on iron overload induced liver injury, as well as the underlying mechanism based on nitrative stress in rat model. We found that when iron overload rats (500mgiron/kg) were fed baicalin-containing diet (0.3% and 1% w/w) for 45days, baicalin dose dependently protected against iron overload induced liver injury, including alleviation of hepatic pathological damage, decrease of SOD activity, iron content, carbonyl content, and the thiobarbituric acid-reactive substances level in hepatic tissues. It also increased serum iron content, SH content and GPx activity, decreased serum ALT and AST activities. Immunohistochemistry and immunoprecipitation analysis revealed that baicalin could also inhibit iron overload induced protein tyrosine nitration in liver. Moreover, in iron overload rat liver, we found that baicalin decreased the iron overload increased level of glutathione-S-transferases (GSTs) expression, oxidation and nitration. These results suggest that not only oxidative stress, but also nitrative stress, is involved in iron overload induced liver injury, and the underlying mechanism might partially relate to the involvement of GSTs expression and post-translational modification. Baicalin can effectively prevent iron overload caused abnormality and can be a candidate medicine for iron overload diseases. SN - 1879-0712 UR - https://www.unboundmedicine.com/medline/citation/22306240/Iron_overload_induced_rat_liver_injury:_Involvement_of_protein_tyrosine_nitration_and_the_effect_of_baicalin_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(12)00061-1 DB - PRIME DP - Unbound Medicine ER -