Tags

Type your tag names separated by a space and hit enter

The effects of neuregulin-1β on neuronal phenotypes of primary cultured dorsal root ganglion neurons by activation of PI3K/Akt.
Neurosci Lett. 2012 Mar 05; 511(1):52-7.NL

Abstract

Neuregulin-1β (NRG-1β) signaling has multiple functions in neurons. NRG-1 signaling regulates neuronal development, migration, myelination, and synaptic maintenance. The neuropeptide- and neurofilament (NF)-immunoreactive (IR) neurons are two major phenotypical classes in dorsal root ganglion (DRG). Whether NRG-1β influences DRG neuronal phenotypes remains unknown. To assess the effects of NRG-1β on DRG neuronal phenotypes, dissociated embryonic rat DRG neuronal culture model was established. Primary cultured DRG neurons were exposed to NRG-1β (5nmol/L), NRG-1β (10nmol/L), NRG-1β (20nmol/L), NRG-1β (20nmol/L) plus LY294002 (10μmol/L) for 3 days, respectively. The DRG neurons were continuously exposed to growth media as control. After that, all above cultured DRG neurons were processed for double fluorescent labeling of calcitonin gene-related peptide (CGRP) or neurofilament-200 (NF-200) and microtubule associated protein 2 (MAP2). The percentage of CGRP-IR neurons and NF-200-IR neurons was counted. The expression of CGRP mRNA and NF-200 mRNA was analyzed by real time-PCR analysis. The percentage of CGRP-IR neurons but not NF-200-IR neurons increased significantly in the presence of NRG-1β as compared with that in the absence of NRG-1β. The levels of CGRP mRNA but not NF-200 mRNA increased significantly in the presence of NRG-1β as compared with that in the absence of NRG-1β. PI3K inhibitor LY294002 blocked the effects of NRG-1β. These results support an important role for exogenous NRG-1β in induction of the distinct neuronal phenotype response by activation of PI3K/Akt in sensory neurons.

Authors+Show Affiliations

Department of Anatomy, Shandong University School of Medicine, Jinan 250012, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22306613

Citation

Liu, Zhen, et al. "The Effects of Neuregulin-1β On Neuronal Phenotypes of Primary Cultured Dorsal Root Ganglion Neurons By Activation of PI3K/Akt." Neuroscience Letters, vol. 511, no. 1, 2012, pp. 52-7.
Liu Z, Jiang H, Li H, et al. The effects of neuregulin-1β on neuronal phenotypes of primary cultured dorsal root ganglion neurons by activation of PI3K/Akt. Neurosci Lett. 2012;511(1):52-7.
Liu, Z., Jiang, H., Li, H., Liu, H., Xu, X., & Li, Z. (2012). The effects of neuregulin-1β on neuronal phenotypes of primary cultured dorsal root ganglion neurons by activation of PI3K/Akt. Neuroscience Letters, 511(1), 52-7. https://doi.org/10.1016/j.neulet.2012.01.041
Liu Z, et al. The Effects of Neuregulin-1β On Neuronal Phenotypes of Primary Cultured Dorsal Root Ganglion Neurons By Activation of PI3K/Akt. Neurosci Lett. 2012 Mar 5;511(1):52-7. PubMed PMID: 22306613.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effects of neuregulin-1β on neuronal phenotypes of primary cultured dorsal root ganglion neurons by activation of PI3K/Akt. AU - Liu,Zhen, AU - Jiang,Hao, AU - Li,Hao, AU - Liu,Huaxiang, AU - Xu,Xiaobo, AU - Li,Zhenzhong, Y1 - 2012/01/25/ PY - 2011/08/30/received PY - 2012/01/16/revised PY - 2012/01/18/accepted PY - 2012/2/7/entrez PY - 2012/2/7/pubmed PY - 2012/7/28/medline SP - 52 EP - 7 JF - Neuroscience letters JO - Neurosci Lett VL - 511 IS - 1 N2 - Neuregulin-1β (NRG-1β) signaling has multiple functions in neurons. NRG-1 signaling regulates neuronal development, migration, myelination, and synaptic maintenance. The neuropeptide- and neurofilament (NF)-immunoreactive (IR) neurons are two major phenotypical classes in dorsal root ganglion (DRG). Whether NRG-1β influences DRG neuronal phenotypes remains unknown. To assess the effects of NRG-1β on DRG neuronal phenotypes, dissociated embryonic rat DRG neuronal culture model was established. Primary cultured DRG neurons were exposed to NRG-1β (5nmol/L), NRG-1β (10nmol/L), NRG-1β (20nmol/L), NRG-1β (20nmol/L) plus LY294002 (10μmol/L) for 3 days, respectively. The DRG neurons were continuously exposed to growth media as control. After that, all above cultured DRG neurons were processed for double fluorescent labeling of calcitonin gene-related peptide (CGRP) or neurofilament-200 (NF-200) and microtubule associated protein 2 (MAP2). The percentage of CGRP-IR neurons and NF-200-IR neurons was counted. The expression of CGRP mRNA and NF-200 mRNA was analyzed by real time-PCR analysis. The percentage of CGRP-IR neurons but not NF-200-IR neurons increased significantly in the presence of NRG-1β as compared with that in the absence of NRG-1β. The levels of CGRP mRNA but not NF-200 mRNA increased significantly in the presence of NRG-1β as compared with that in the absence of NRG-1β. PI3K inhibitor LY294002 blocked the effects of NRG-1β. These results support an important role for exogenous NRG-1β in induction of the distinct neuronal phenotype response by activation of PI3K/Akt in sensory neurons. SN - 1872-7972 UR - https://www.unboundmedicine.com/medline/citation/22306613/The_effects_of_neuregulin_1β_on_neuronal_phenotypes_of_primary_cultured_dorsal_root_ganglion_neurons_by_activation_of_PI3K/Akt_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3940(12)00096-1 DB - PRIME DP - Unbound Medicine ER -