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The anti-Candida activity of Thymbra capitata essential oil: effect upon pre-formed biofilm.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

[corrected] Thymbra capitata essential oil is traditionally considered to exhibit powerful antiseptic properties, thus being used to treat cutaneous infections. The aim of the present study was to evaluate the effect of Thymbra capitata essential oil upon pre-formed biofilm of different Candida strains while comparing it with the activity against planktonic cells.

MATERIALS AND METHODS

Fifteen Candida isolates were included, corresponding to clinical and collection type strains. Essential oil was obtained by hydrodistillation and its composition analysed by GC/MS. Activity upon planktonic cells was evaluated according to M27-A3 macromethod. Its effect upon 24h preformed biofilm biomass was determined using the crystal violet procedure and the metabolic activity was studied applying the XTT/menadione technique.

RESULTS

Biofilm biomass and metabolic activity of all tested species were reduced up to 50% at MIC values. The effect was more pronounced at double MIC values, achieving >80% reduction, except for Candida albicans that presented a more resistant profile (62%).

CONCLUSION

Thymbra capitata essential oil presented an important effect upon Candida biofilms. It is proposed as a valuable antifungal product to be used in an appropriate pharmaceutical formulation for the management of resistant mucocutaneous candidosis.

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  • Authors+Show Affiliations

    ,

    CICS-UBI, Health Sciences Research Center, Faculty of Health Sciences, University of Beira Interior, Covilhã, Portugal. apo@fcsaude.ubi.pt

    , , , , , , , ,

    Source

    Journal of ethnopharmacology 140:2 2012 Mar 27 pg 379-83

    MeSH

    Antifungal Agents
    Biofilms
    Biomass
    Candida
    Candida albicans
    Gentian Violet
    Lamiaceae
    Microbial Sensitivity Tests
    Oils, Volatile
    Plankton
    Plant Extracts

    Pub Type(s)

    Comparative Study
    Journal Article

    Language

    eng

    PubMed ID

    22310557

    Citation

    Palmeira-de-Oliveira, A, et al. "The anti-Candida Activity of Thymbra Capitata Essential Oil: Effect Upon Pre-formed Biofilm." Journal of Ethnopharmacology, vol. 140, no. 2, 2012, pp. 379-83.
    Palmeira-de-Oliveira A, Gaspar C, Palmeira-de-Oliveira R, et al. The anti-Candida activity of Thymbra capitata essential oil: effect upon pre-formed biofilm. J Ethnopharmacol. 2012;140(2):379-83.
    Palmeira-de-Oliveira, A., Gaspar, C., Palmeira-de-Oliveira, R., Silva-Dias, A., Salgueiro, L., Cavaleiro, C., ... Rodrigues, A. G. (2012). The anti-Candida activity of Thymbra capitata essential oil: effect upon pre-formed biofilm. Journal of Ethnopharmacology, 140(2), pp. 379-83. doi:10.1016/j.jep.2012.01.029.
    Palmeira-de-Oliveira A, et al. The anti-Candida Activity of Thymbra Capitata Essential Oil: Effect Upon Pre-formed Biofilm. J Ethnopharmacol. 2012 Mar 27;140(2):379-83. PubMed PMID: 22310557.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - The anti-Candida activity of Thymbra capitata essential oil: effect upon pre-formed biofilm. AU - Palmeira-de-Oliveira,A, AU - Gaspar,C, AU - Palmeira-de-Oliveira,R, AU - Silva-Dias,A, AU - Salgueiro,L, AU - Cavaleiro,C, AU - Pina-Vaz,C, AU - Martinez-de-Oliveira,J, AU - Queiroz,J A, AU - Rodrigues,A G, Y1 - 2012/01/28/ PY - 2011/10/11/received PY - 2012/01/02/revised PY - 2012/01/18/accepted PY - 2012/2/8/entrez PY - 2012/2/9/pubmed PY - 2012/8/3/medline SP - 379 EP - 83 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 140 IS - 2 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: [corrected] Thymbra capitata essential oil is traditionally considered to exhibit powerful antiseptic properties, thus being used to treat cutaneous infections. The aim of the present study was to evaluate the effect of Thymbra capitata essential oil upon pre-formed biofilm of different Candida strains while comparing it with the activity against planktonic cells. MATERIALS AND METHODS: Fifteen Candida isolates were included, corresponding to clinical and collection type strains. Essential oil was obtained by hydrodistillation and its composition analysed by GC/MS. Activity upon planktonic cells was evaluated according to M27-A3 macromethod. Its effect upon 24h preformed biofilm biomass was determined using the crystal violet procedure and the metabolic activity was studied applying the XTT/menadione technique. RESULTS: Biofilm biomass and metabolic activity of all tested species were reduced up to 50% at MIC values. The effect was more pronounced at double MIC values, achieving >80% reduction, except for Candida albicans that presented a more resistant profile (62%). CONCLUSION: Thymbra capitata essential oil presented an important effect upon Candida biofilms. It is proposed as a valuable antifungal product to be used in an appropriate pharmaceutical formulation for the management of resistant mucocutaneous candidosis. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/22310557/The_anti_Candida_activity_of_Thymbra_capitata_essential_oil:_effect_upon_pre_formed_biofilm_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(12)00042-6 DB - PRIME DP - Unbound Medicine ER -