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Wild animal surveillance for coronavirus HKU1 and potential variants of other coronaviruses.
Hong Kong Med J. 2012 Feb; 18 Suppl 2:25-6.HK

Abstract

1. Although CoV-HKU1 was not identified in any of the studied animals, a coronavirus closely related to SARS-CoV (bat-SARS-CoV) was identified in 23 (19%) of 118 wild Chinese horseshoe bats by reverse transcriptase polymerase chain reaction (RT-PCR). 2. Complete genome sequencing and phylogenetic analysis showed that bat-SARS-CoV formed a distinct cluster with SARS-CoV as group 2b coronaviruses, distantly related to known group 2 coronaviruses. 3. Most differences between the bat-SARS-CoV and SARS-CoV genomes were observed in the spike gene. The presence of a29-bp insertion in ORF 8 of bat-SARS-CoV genome, not in most human SARS-CoV genomes, suggests that it has a common ancestor with civet SARS-CoV. 4. Antibody against recombinant bat-SARS-CoV nucleocapsid protein was detected in 84% of Chinese horseshoe bats using an enzyme immunoassay.Neutralising antibody to human SARS-CoV was also detected in those with lower viral loads.5. This study also revealed a previously unknown diversity of coronaviruses in bats, which are important natural reservoir for coronaviruses including SARS-CoV-like viruses.

Authors+Show Affiliations

Department of Microbiology, The University of Hong Kong. Queen Mary Hospital, Hong Kong SAR, China. hkumicro@hkucc.hku.hkNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22311357

Citation

Yuen, K Y., et al. "Wild Animal Surveillance for Coronavirus HKU1 and Potential Variants of Other Coronaviruses." Hong Kong Medical Journal = Xianggang Yi Xue Za Zhi, vol. 18 Suppl 2, 2012, pp. 25-6.
Yuen KY, Lau SK, Woo PC. Wild animal surveillance for coronavirus HKU1 and potential variants of other coronaviruses. Hong Kong Med J. 2012;18 Suppl 2:25-6.
Yuen, K. Y., Lau, S. K., & Woo, P. C. (2012). Wild animal surveillance for coronavirus HKU1 and potential variants of other coronaviruses. Hong Kong Medical Journal = Xianggang Yi Xue Za Zhi, 18 Suppl 2, 25-6.
Yuen KY, Lau SK, Woo PC. Wild Animal Surveillance for Coronavirus HKU1 and Potential Variants of Other Coronaviruses. Hong Kong Med J. 2012;18 Suppl 2:25-6. PubMed PMID: 22311357.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Wild animal surveillance for coronavirus HKU1 and potential variants of other coronaviruses. AU - Yuen,K Y, AU - Lau,S K P, AU - Woo,P C Y, PY - 2012/2/8/entrez PY - 2012/2/15/pubmed PY - 2012/5/31/medline SP - 25 EP - 6 JF - Hong Kong medical journal = Xianggang yi xue za zhi JO - Hong Kong Med J VL - 18 Suppl 2 N2 - 1. Although CoV-HKU1 was not identified in any of the studied animals, a coronavirus closely related to SARS-CoV (bat-SARS-CoV) was identified in 23 (19%) of 118 wild Chinese horseshoe bats by reverse transcriptase polymerase chain reaction (RT-PCR). 2. Complete genome sequencing and phylogenetic analysis showed that bat-SARS-CoV formed a distinct cluster with SARS-CoV as group 2b coronaviruses, distantly related to known group 2 coronaviruses. 3. Most differences between the bat-SARS-CoV and SARS-CoV genomes were observed in the spike gene. The presence of a29-bp insertion in ORF 8 of bat-SARS-CoV genome, not in most human SARS-CoV genomes, suggests that it has a common ancestor with civet SARS-CoV. 4. Antibody against recombinant bat-SARS-CoV nucleocapsid protein was detected in 84% of Chinese horseshoe bats using an enzyme immunoassay.Neutralising antibody to human SARS-CoV was also detected in those with lower viral loads.5. This study also revealed a previously unknown diversity of coronaviruses in bats, which are important natural reservoir for coronaviruses including SARS-CoV-like viruses. SN - 1024-2708 UR - https://www.unboundmedicine.com/medline/citation/22311357/Wild_animal_surveillance_for_coronavirus_HKU1_and_potential_variants_of_other_coronaviruses_ L2 - http://www.hkmj.org/abstracts/v18n1s2/25.htm DB - PRIME DP - Unbound Medicine ER -