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Determination of phenolic acids and flavonoids in Taraxacum formosanum Kitam by liquid chromatography-tandem mass spectrometry coupled with a post-column derivatization technique.
Int J Mol Sci. 2012; 13(1):260-85.IJ

Abstract

A liquid chromatography-tandem mass spectrometry method (LC-MS/MS) was developed for the determination of phenolic acids and flavonoids in a medicinal Chinese herb Taraxacum formosanum Kitam. Initially, both phenolic acids and flavonoids were extracted with 50% ethanol in a water-bath at 60 °C for 3 h and eventually separated into acidic fraction and neutral fraction by using a C(18) cartridge. A total of 29 compounds were separated within 68 min by employing a Gemini C(18) column and a gradient solvent system of 0.1% formic acid and acetonitrile at a flow rate of 1.0 mL/min. Based on the retention behavior as well as absorption and mass spectra, 19 phenolic acids and 10 flavonoids were identified and quantified in T. formosanum, with the former ranging from 14.1 μg/g to 10,870.4 μg/g, and the latter from 9.9 μg/g to 325.8 μg/g. For further identification of flavonoids, a post-column derivatization method involving shift reagents such as sodium acetate or aluminum chloride was used and the absorption spectral characteristics without or with shift reagents were compared. An internal standard syringic acid was used for quantitation of phenolic acids, whereas (±) naringenin was found suitable for quantitation of flavonoids. The developed LC-MS/MS method showed high reproducibility, as evident from the relative standard deviation (RSD) values for intra-day and inter-day variability being 1.0-6.8% and 2.0-7.7% for phenolic acids and 3.7-7.4% and 1.5-8.1% for flavonoids, respectively, and thus may be applied for simultaneous determination of phenolic acids and flavonoids in Chinese herb and nutraceuticals.

Authors+Show Affiliations

Department of Food Science, Fu Jen University, Taipei 242, Taiwan; E-Mails: a29939245@hotmail.com (H.-J.C.); sinbaraj@yahoo.com (B.S.I.).No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22312251

Citation

Chen, Hung-Ju, et al. "Determination of Phenolic Acids and Flavonoids in Taraxacum Formosanum Kitam By Liquid Chromatography-tandem Mass Spectrometry Coupled With a Post-column Derivatization Technique." International Journal of Molecular Sciences, vol. 13, no. 1, 2012, pp. 260-85.
Chen HJ, Inbaraj BS, Chen BH. Determination of phenolic acids and flavonoids in Taraxacum formosanum Kitam by liquid chromatography-tandem mass spectrometry coupled with a post-column derivatization technique. Int J Mol Sci. 2012;13(1):260-85.
Chen, H. J., Inbaraj, B. S., & Chen, B. H. (2012). Determination of phenolic acids and flavonoids in Taraxacum formosanum Kitam by liquid chromatography-tandem mass spectrometry coupled with a post-column derivatization technique. International Journal of Molecular Sciences, 13(1), 260-85. https://doi.org/10.3390/ijms13010260
Chen HJ, Inbaraj BS, Chen BH. Determination of Phenolic Acids and Flavonoids in Taraxacum Formosanum Kitam By Liquid Chromatography-tandem Mass Spectrometry Coupled With a Post-column Derivatization Technique. Int J Mol Sci. 2012;13(1):260-85. PubMed PMID: 22312251.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of phenolic acids and flavonoids in Taraxacum formosanum Kitam by liquid chromatography-tandem mass spectrometry coupled with a post-column derivatization technique. AU - Chen,Hung-Ju, AU - Inbaraj,Baskaran Stephen, AU - Chen,Bing-Huei, Y1 - 2011/12/27/ PY - 2011/10/28/received PY - 2011/11/25/revised PY - 2011/12/12/accepted PY - 2012/2/8/entrez PY - 2012/2/9/pubmed PY - 2012/2/9/medline KW - LC-MS-MS KW - Taraxacum formosanum Kitam KW - flavonoid KW - phenolic acid SP - 260 EP - 85 JF - International journal of molecular sciences JO - Int J Mol Sci VL - 13 IS - 1 N2 - A liquid chromatography-tandem mass spectrometry method (LC-MS/MS) was developed for the determination of phenolic acids and flavonoids in a medicinal Chinese herb Taraxacum formosanum Kitam. Initially, both phenolic acids and flavonoids were extracted with 50% ethanol in a water-bath at 60 °C for 3 h and eventually separated into acidic fraction and neutral fraction by using a C(18) cartridge. A total of 29 compounds were separated within 68 min by employing a Gemini C(18) column and a gradient solvent system of 0.1% formic acid and acetonitrile at a flow rate of 1.0 mL/min. Based on the retention behavior as well as absorption and mass spectra, 19 phenolic acids and 10 flavonoids were identified and quantified in T. formosanum, with the former ranging from 14.1 μg/g to 10,870.4 μg/g, and the latter from 9.9 μg/g to 325.8 μg/g. For further identification of flavonoids, a post-column derivatization method involving shift reagents such as sodium acetate or aluminum chloride was used and the absorption spectral characteristics without or with shift reagents were compared. An internal standard syringic acid was used for quantitation of phenolic acids, whereas (±) naringenin was found suitable for quantitation of flavonoids. The developed LC-MS/MS method showed high reproducibility, as evident from the relative standard deviation (RSD) values for intra-day and inter-day variability being 1.0-6.8% and 2.0-7.7% for phenolic acids and 3.7-7.4% and 1.5-8.1% for flavonoids, respectively, and thus may be applied for simultaneous determination of phenolic acids and flavonoids in Chinese herb and nutraceuticals. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/22312251/Determination_of_phenolic_acids_and_flavonoids_in_Taraxacum_formosanum_Kitam_by_liquid_chromatography_tandem_mass_spectrometry_coupled_with_a_post_column_derivatization_technique_ L2 - https://doi.org/10.3390/ijms13010260 DB - PRIME DP - Unbound Medicine ER -