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Bioactive compounds, RP-HPLC analysis of phenolics, and antioxidant activity of some Portuguese shrub species extracts.
Nat Prod Commun. 2011 Dec; 6(12):1863-72.NP

Abstract

In the ecosystem of Serra Da Estrela, some plant species have the potential to be used as raw material for extraction of bioactive products. The goal of this work was to determine the phenolic, flavonoid, tannin and alkaloid contents of the methanolic extracts of some shrubs (Echinospartum ibericum, Pterospartum tridentatum, Juniperus communis, Ruscus aculeatus, Rubus ulmifolius, Hakea sericea, Cytisus multiflorus, Crataegus monogyna, Erica arborea and Ipomoea acuminata), and then to correlate the phenolic compounds and flavonoids with the antioxidant activity of each extract. The Folin-Ciocalteu's method was used for the determination of total phenols, and tannins were then precipitated with polyvinylpolypyrrolidone (PVPP); a colorimetric method with aluminum chloride was used for the determination of flavonoids, and a Dragendorff's reagent method was used for total alkaloid estimation. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and beta-carotene bleaching tests were used to assess the antioxidant activity of extracts. The identification of phenolic compounds present in extracts was performed using RP-HPLC. A positive linear correlation between antioxidant activity index and total phenolic content of methanolic extracts was observed. The RP-HPLC procedure showed that the most common compounds were ferulic and ellagic acids and quercetin. Most of the studied shrubs have significant antioxidant properties that are probably due to the existence of phenolic compounds in the extracts. It is noteworthy to emphasize that for Echinospartum ibericum, Hakea sericea and Ipomoea acuminata, to the best of our knowledge, no phytochemical studies have been undertaken nor their use in traditional medicine been described.

Authors+Show Affiliations

Luís A
CICS-UBI Health Sciences Research Centre, University of Beira Interior, Av. Infante D. Henrique 6200-506 Covilhã, Portugal.
Domingues F
No affiliation info available
Duarte AP
No affiliation info available

MeSH

AntioxidantsChromatography, High Pressure LiquidFlavonoidsPhenolsPlant ExtractsPortugal

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22312726

Citation

Luís, Angelo, et al. "Bioactive Compounds, RP-HPLC Analysis of Phenolics, and Antioxidant Activity of some Portuguese Shrub Species Extracts." Natural Product Communications, vol. 6, no. 12, 2011, pp. 1863-72.
Luís A, Domingues F, Duarte AP. Bioactive compounds, RP-HPLC analysis of phenolics, and antioxidant activity of some Portuguese shrub species extracts. Nat Prod Commun. 2011;6(12):1863-72.
Luís, A., Domingues, F., & Duarte, A. P. (2011). Bioactive compounds, RP-HPLC analysis of phenolics, and antioxidant activity of some Portuguese shrub species extracts. Natural Product Communications, 6(12), 1863-72.
Luís A, Domingues F, Duarte AP. Bioactive Compounds, RP-HPLC Analysis of Phenolics, and Antioxidant Activity of some Portuguese Shrub Species Extracts. Nat Prod Commun. 2011;6(12):1863-72. PubMed PMID: 22312726.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bioactive compounds, RP-HPLC analysis of phenolics, and antioxidant activity of some Portuguese shrub species extracts. AU - Luís,Angelo, AU - Domingues,Fernanda, AU - Duarte,Ana Paula, PY - 2012/2/9/entrez PY - 2012/2/9/pubmed PY - 2012/5/4/medline SP - 1863 EP - 72 JF - Natural product communications JO - Nat Prod Commun VL - 6 IS - 12 N2 - In the ecosystem of Serra Da Estrela, some plant species have the potential to be used as raw material for extraction of bioactive products. The goal of this work was to determine the phenolic, flavonoid, tannin and alkaloid contents of the methanolic extracts of some shrubs (Echinospartum ibericum, Pterospartum tridentatum, Juniperus communis, Ruscus aculeatus, Rubus ulmifolius, Hakea sericea, Cytisus multiflorus, Crataegus monogyna, Erica arborea and Ipomoea acuminata), and then to correlate the phenolic compounds and flavonoids with the antioxidant activity of each extract. The Folin-Ciocalteu's method was used for the determination of total phenols, and tannins were then precipitated with polyvinylpolypyrrolidone (PVPP); a colorimetric method with aluminum chloride was used for the determination of flavonoids, and a Dragendorff's reagent method was used for total alkaloid estimation. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and beta-carotene bleaching tests were used to assess the antioxidant activity of extracts. The identification of phenolic compounds present in extracts was performed using RP-HPLC. A positive linear correlation between antioxidant activity index and total phenolic content of methanolic extracts was observed. The RP-HPLC procedure showed that the most common compounds were ferulic and ellagic acids and quercetin. Most of the studied shrubs have significant antioxidant properties that are probably due to the existence of phenolic compounds in the extracts. It is noteworthy to emphasize that for Echinospartum ibericum, Hakea sericea and Ipomoea acuminata, to the best of our knowledge, no phytochemical studies have been undertaken nor their use in traditional medicine been described. SN - 1934-578X UR - https://www.unboundmedicine.com/medline/citation/22312726/Bioactive_compounds_RP_HPLC_analysis_of_phenolics_and_antioxidant_activity_of_some_Portuguese_shrub_species_extracts_ L2 - https://medlineplus.gov/antioxidants.html DB - PRIME DP - Unbound Medicine ER -