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Functional dysbiosis within the gut microbiota of patients with constipated-irritable bowel syndrome.

Abstract

BACKGROUND

The role of the gut microbiota in patho-physiology of irritable bowel syndrome (IBS) is suggested by several studies. However, standard cultural and molecular methods used to date have not revealed specific and consistent IBS-related groups of microbes.

AIM

To explore the constipated-IBS (C-IBS) gut microbiota using a function-based approach.

METHODS

The faecal microbiota from 14 C-IBS women and 12 sex-match healthy subjects were examined through a combined strictly anaerobic cultural evaluation of functional groups of microbes and fluorescent in situ hybridisation (16S rDNA gene targeting probes) to quantify main groups of bacteria. Starch fermentation by C-IBS and healthy faecal samples was evaluated in vitro.

RESULTS

In C-IBS, the numbers of lactate-producing and lactate-utilising bacteria and the number of H(2) -consuming populations, methanogens and reductive acetogens, were at least 10-fold lower (P < 0.05) compared with control subjects. Concomitantly, the number of lactate- and H(2) -utilising sulphate-reducing population was 10 to 100 fold increased in C-IBS compared with healthy subjects. The butyrate-producing Roseburia - E. rectale group was in lower number (0.01 < P < 0.05) in C-IBS than in control. C-IBS faecal microbiota produced more sulphides and H(2) and less butyrate from starch fermentation than healthy ones.

CONCLUSIONS

A major functional dysbiosis was observed in constipated-irritable bowel syndrome gut microbiota, reflecting altered intestinal fermentation. Sulphate-reducing population increased in the gut of C-IBS and were accompanied by alterations in other microbial groups. This could be responsible for changes in the metabolic output and enhancement in toxic sulphide production which could in turn influence gut physiology and contribute to IBS pathogenesis.

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  • Authors+Show Affiliations

    ,

    INRA, UR Microbiology Unit, Clermont-Ferrand Research Centre, Saint Genès-Champanelle, France.

    , , , , , , , , , , ,

    Source

    MeSH

    Adult
    Case-Control Studies
    Constipation
    Feces
    Female
    Gastrointestinal Tract
    Humans
    In Situ Hybridization, Fluorescence
    Irritable Bowel Syndrome
    Male
    Metagenome
    Middle Aged
    Young Adult

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    22315951

    Citation

    Chassard, C, et al. "Functional Dysbiosis Within the Gut Microbiota of Patients With Constipated-irritable Bowel Syndrome." Alimentary Pharmacology & Therapeutics, vol. 35, no. 7, 2012, pp. 828-38.
    Chassard C, Dapoigny M, Scott KP, et al. Functional dysbiosis within the gut microbiota of patients with constipated-irritable bowel syndrome. Aliment Pharmacol Ther. 2012;35(7):828-38.
    Chassard, C., Dapoigny, M., Scott, K. P., Crouzet, L., Del'homme, C., Marquet, P., ... Bernalier-Donadille, A. (2012). Functional dysbiosis within the gut microbiota of patients with constipated-irritable bowel syndrome. Alimentary Pharmacology & Therapeutics, 35(7), pp. 828-38. doi:10.1111/j.1365-2036.2012.05007.x.
    Chassard C, et al. Functional Dysbiosis Within the Gut Microbiota of Patients With Constipated-irritable Bowel Syndrome. Aliment Pharmacol Ther. 2012;35(7):828-38. PubMed PMID: 22315951.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Functional dysbiosis within the gut microbiota of patients with constipated-irritable bowel syndrome. AU - Chassard,C, AU - Dapoigny,M, AU - Scott,K P, AU - Crouzet,L, AU - Del'homme,C, AU - Marquet,P, AU - Martin,J C, AU - Pickering,G, AU - Ardid,D, AU - Eschalier,A, AU - Dubray,C, AU - Flint,H J, AU - Bernalier-Donadille,A, Y1 - 2012/02/08/ PY - 2011/08/26/received PY - 2011/09/20/revised PY - 2012/01/12/revised PY - 2012/01/12/accepted PY - 2012/2/10/entrez PY - 2012/2/10/pubmed PY - 2012/6/1/medline SP - 828 EP - 38 JF - Alimentary pharmacology & therapeutics JO - Aliment. Pharmacol. Ther. VL - 35 IS - 7 N2 - BACKGROUND: The role of the gut microbiota in patho-physiology of irritable bowel syndrome (IBS) is suggested by several studies. However, standard cultural and molecular methods used to date have not revealed specific and consistent IBS-related groups of microbes. AIM: To explore the constipated-IBS (C-IBS) gut microbiota using a function-based approach. METHODS: The faecal microbiota from 14 C-IBS women and 12 sex-match healthy subjects were examined through a combined strictly anaerobic cultural evaluation of functional groups of microbes and fluorescent in situ hybridisation (16S rDNA gene targeting probes) to quantify main groups of bacteria. Starch fermentation by C-IBS and healthy faecal samples was evaluated in vitro. RESULTS: In C-IBS, the numbers of lactate-producing and lactate-utilising bacteria and the number of H(2) -consuming populations, methanogens and reductive acetogens, were at least 10-fold lower (P < 0.05) compared with control subjects. Concomitantly, the number of lactate- and H(2) -utilising sulphate-reducing population was 10 to 100 fold increased in C-IBS compared with healthy subjects. The butyrate-producing Roseburia - E. rectale group was in lower number (0.01 < P < 0.05) in C-IBS than in control. C-IBS faecal microbiota produced more sulphides and H(2) and less butyrate from starch fermentation than healthy ones. CONCLUSIONS: A major functional dysbiosis was observed in constipated-irritable bowel syndrome gut microbiota, reflecting altered intestinal fermentation. Sulphate-reducing population increased in the gut of C-IBS and were accompanied by alterations in other microbial groups. This could be responsible for changes in the metabolic output and enhancement in toxic sulphide production which could in turn influence gut physiology and contribute to IBS pathogenesis. SN - 1365-2036 UR - https://www.unboundmedicine.com/medline/citation/22315951/Functional_dysbiosis_within_the_gut_microbiota_of_patients_with_constipated_irritable_bowel_syndrome_ L2 - https://doi.org/10.1111/j.1365-2036.2012.05007.x DB - PRIME DP - Unbound Medicine ER -