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Simple analysis of naphthalene, fluorene, and anthracene in whole blood by gas chromatography/mass spectrometry after headspace-solid phase microextraction.
J AOAC Int. 2011 Nov-Dec; 94(6):1891-5.JA

Abstract

A simple and sensitive method for the simultaneous analysis of naphthalene, fluorene, and anthracene in whole blood was developed using headspace-solid-phase microextraction (SPME) and GC/MS. A 0.5 g whole blood sample, 5 microL naphthalene, fluorene, and anthracene (50 microg/mL) as spiked standards, and 0.5 mL sodium hydroxide were placed into a 12 mL vial and sealed rapidly. The vial was immediately heated to 70 degrees C in an aluminium block heater, the needle of the SPME device was inserted through the septum of the vial, and the extraction fiber was exposed to the headspace for 15 min. Afterwards, the compounds extracted by the fiber were desorbed simultaneously by exposing the fiber in the gas chromatograph injection port. No interferences were found, and the time for analysis was about 30 min for one sample. This method was applied to a suicide case in which the victim ingested naphthalene, fluorene, and anthracene.

Authors+Show Affiliations

Pamukkale University, Faculty of Arts and Sciences, Department of Biochemistry, 20017 Denizli, Turkey. hardagakdogan@gmail.com

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22320097

Citation

Akdoğan, Hatice Ardag. "Simple Analysis of Naphthalene, Fluorene, and Anthracene in Whole Blood By Gas Chromatography/mass Spectrometry After Headspace-solid Phase Microextraction." Journal of AOAC International, vol. 94, no. 6, 2011, pp. 1891-5.
Akdoğan HA. Simple analysis of naphthalene, fluorene, and anthracene in whole blood by gas chromatography/mass spectrometry after headspace-solid phase microextraction. J AOAC Int. 2011;94(6):1891-5.
Akdoğan, H. A. (2011). Simple analysis of naphthalene, fluorene, and anthracene in whole blood by gas chromatography/mass spectrometry after headspace-solid phase microextraction. Journal of AOAC International, 94(6), 1891-5.
Akdoğan HA. Simple Analysis of Naphthalene, Fluorene, and Anthracene in Whole Blood By Gas Chromatography/mass Spectrometry After Headspace-solid Phase Microextraction. J AOAC Int. 2011 Nov-Dec;94(6):1891-5. PubMed PMID: 22320097.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simple analysis of naphthalene, fluorene, and anthracene in whole blood by gas chromatography/mass spectrometry after headspace-solid phase microextraction. A1 - Akdoğan,Hatice Ardag, PY - 2012/2/11/entrez PY - 2012/2/11/pubmed PY - 2012/3/9/medline SP - 1891 EP - 5 JF - Journal of AOAC International JO - J AOAC Int VL - 94 IS - 6 N2 - A simple and sensitive method for the simultaneous analysis of naphthalene, fluorene, and anthracene in whole blood was developed using headspace-solid-phase microextraction (SPME) and GC/MS. A 0.5 g whole blood sample, 5 microL naphthalene, fluorene, and anthracene (50 microg/mL) as spiked standards, and 0.5 mL sodium hydroxide were placed into a 12 mL vial and sealed rapidly. The vial was immediately heated to 70 degrees C in an aluminium block heater, the needle of the SPME device was inserted through the septum of the vial, and the extraction fiber was exposed to the headspace for 15 min. Afterwards, the compounds extracted by the fiber were desorbed simultaneously by exposing the fiber in the gas chromatograph injection port. No interferences were found, and the time for analysis was about 30 min for one sample. This method was applied to a suicide case in which the victim ingested naphthalene, fluorene, and anthracene. SN - 1060-3271 UR - https://www.unboundmedicine.com/medline/citation/22320097/Simple_analysis_of_naphthalene_fluorene_and_anthracene_in_whole_blood_by_gas_chromatography/mass_spectrometry_after_headspace_solid_phase_microextraction_ L2 - https://academic.oup.com/jaoac/article-lookup/doi/10.5740/jaoacint.11-124 DB - PRIME DP - Unbound Medicine ER -