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Novel magnetic Fe3O4@CdSe composite quantum dot-based electrochemiluminescence detection of thrombin by a multiple DNA cycle amplification strategy.
Anal Chem. 2012 Mar 20; 84(6):2811-7.AC

Abstract

A novel small magnetic electrochemiluminescent Fe(3)O(4)@CdSe composite quantum dot (QD) was facilely prepared and successfully applied to sensitive electrochemiluminescence (ECL) detection of thrombin by a multiple DNA cycle amplification strategy for the first time. The as-prepared composite QDs feature intense ECL, excellent magnetism, strong fluorescence, and favorable biocompatibility, which offers promising advantages for ECL biosensing. ECL of the composite QDs was efficiently quenched by gold nanoparticles (NPs). Taking advantages of the unique and attractive ECL and magnetic characteristics of the composite QDs, a novel DNA-amplified detection method based on ECL quenching was thus developed for a sensitive assay of thrombin. More importantly, the DNA devices by cleavage reaction were cycled multiple rounds, which greatly amplified the ECL signal and much improve the detection sensitivity. This flexible biosensing system exhibits not only high sensitivity and specificity but also excellent performance in real human serum assay. The present work opens a promising approach to develop magnetic quantum dot-based amplified ECL bioassays, which has wider potential application with more favorable analytical performances than other ECL reagent-based systems. Moreover, the composite QDs are suitable for long-term fluorescent cellular imaging, which also highlights the promising directions for further development of QD-based in vitro and in vivo imaging materials.

Authors+Show Affiliations

State Key Laboratory Base of Eco-chemical Engineering, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, China. guifenjie0318@163.comNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22320223

Citation

Jie, Guifen, and Jinxin Yuan. "Novel Magnetic Fe3O4@CdSe Composite Quantum Dot-based Electrochemiluminescence Detection of Thrombin By a Multiple DNA Cycle Amplification Strategy." Analytical Chemistry, vol. 84, no. 6, 2012, pp. 2811-7.
Jie G, Yuan J. Novel magnetic Fe3O4@CdSe composite quantum dot-based electrochemiluminescence detection of thrombin by a multiple DNA cycle amplification strategy. Anal Chem. 2012;84(6):2811-7.
Jie, G., & Yuan, J. (2012). Novel magnetic Fe3O4@CdSe composite quantum dot-based electrochemiluminescence detection of thrombin by a multiple DNA cycle amplification strategy. Analytical Chemistry, 84(6), 2811-7. https://doi.org/10.1021/ac203261x
Jie G, Yuan J. Novel Magnetic Fe3O4@CdSe Composite Quantum Dot-based Electrochemiluminescence Detection of Thrombin By a Multiple DNA Cycle Amplification Strategy. Anal Chem. 2012 Mar 20;84(6):2811-7. PubMed PMID: 22320223.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Novel magnetic Fe3O4@CdSe composite quantum dot-based electrochemiluminescence detection of thrombin by a multiple DNA cycle amplification strategy. AU - Jie,Guifen, AU - Yuan,Jinxin, Y1 - 2012/03/06/ PY - 2012/2/11/entrez PY - 2012/2/11/pubmed PY - 2012/7/24/medline SP - 2811 EP - 7 JF - Analytical chemistry JO - Anal Chem VL - 84 IS - 6 N2 - A novel small magnetic electrochemiluminescent Fe(3)O(4)@CdSe composite quantum dot (QD) was facilely prepared and successfully applied to sensitive electrochemiluminescence (ECL) detection of thrombin by a multiple DNA cycle amplification strategy for the first time. The as-prepared composite QDs feature intense ECL, excellent magnetism, strong fluorescence, and favorable biocompatibility, which offers promising advantages for ECL biosensing. ECL of the composite QDs was efficiently quenched by gold nanoparticles (NPs). Taking advantages of the unique and attractive ECL and magnetic characteristics of the composite QDs, a novel DNA-amplified detection method based on ECL quenching was thus developed for a sensitive assay of thrombin. More importantly, the DNA devices by cleavage reaction were cycled multiple rounds, which greatly amplified the ECL signal and much improve the detection sensitivity. This flexible biosensing system exhibits not only high sensitivity and specificity but also excellent performance in real human serum assay. The present work opens a promising approach to develop magnetic quantum dot-based amplified ECL bioassays, which has wider potential application with more favorable analytical performances than other ECL reagent-based systems. Moreover, the composite QDs are suitable for long-term fluorescent cellular imaging, which also highlights the promising directions for further development of QD-based in vitro and in vivo imaging materials. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/22320223/Novel_magnetic_Fe3O4@CdSe_composite_quantum_dot_based_electrochemiluminescence_detection_of_thrombin_by_a_multiple_DNA_cycle_amplification_strategy_ L2 - https://doi.org/10.1021/ac203261x DB - PRIME DP - Unbound Medicine ER -