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Chemiluminescence imaging immunoassay of multiple tumor markers for cancer screening.
Anal Chem. 2012 Mar 06; 84(5):2410-5.AC

Abstract

A sensitive chemiluminescence (CL) imaging immunoassay method for detection of multiple tumor markers with high throughput, easy operation, and low cost was developed. The immunosensor array was prepared by covalently immobilizing capture antibodies on corresponding sensing sites on a silanized disposable glass chip. Gold nanoparticle-based bioconjugates with a high molar ratio of horseradish peroxidase (HRP) to detection antibodies were used for signal amplification. Under a sandwich immunoassay, the CL signals triggered by HRP captured on each sensing cell were collected by a charge-coupled device for simultaneous measurement of biomarkers and combination diagnosis of certain tumors. As a proof of concept, the immunosensor array was applied to detect α-fetoprotein, carcinoma antigen 125, carbohydrate antigen 153, and carcinoembryonic antigen and to screen patients with liver, breast, or ovarian cancers. This method showed wide linear ranges over 5 orders of magnitude and much lower detection limits than previously reported multiplexed immunoassays. The high throughput and acceptable stability, reproducibility, and accuracy showed good applicability of the proposed multiplex CL imaging immunoassay in clinical diagnosis.

Authors+Show Affiliations

State Key Laboratory of Analytical Chemistry for Life Science, Department of Chemistry, Nanjing University, Nanjing 210093, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22320247

Citation

Zong, Chen, et al. "Chemiluminescence Imaging Immunoassay of Multiple Tumor Markers for Cancer Screening." Analytical Chemistry, vol. 84, no. 5, 2012, pp. 2410-5.
Zong C, Wu J, Wang C, et al. Chemiluminescence imaging immunoassay of multiple tumor markers for cancer screening. Anal Chem. 2012;84(5):2410-5.
Zong, C., Wu, J., Wang, C., Ju, H., & Yan, F. (2012). Chemiluminescence imaging immunoassay of multiple tumor markers for cancer screening. Analytical Chemistry, 84(5), 2410-5. https://doi.org/10.1021/ac203179g
Zong C, et al. Chemiluminescence Imaging Immunoassay of Multiple Tumor Markers for Cancer Screening. Anal Chem. 2012 Mar 6;84(5):2410-5. PubMed PMID: 22320247.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chemiluminescence imaging immunoassay of multiple tumor markers for cancer screening. AU - Zong,Chen, AU - Wu,Jie, AU - Wang,Chen, AU - Ju,Huangxian, AU - Yan,Feng, Y1 - 2012/02/21/ PY - 2012/2/11/entrez PY - 2012/2/11/pubmed PY - 2012/7/31/medline SP - 2410 EP - 5 JF - Analytical chemistry JO - Anal. Chem. VL - 84 IS - 5 N2 - A sensitive chemiluminescence (CL) imaging immunoassay method for detection of multiple tumor markers with high throughput, easy operation, and low cost was developed. The immunosensor array was prepared by covalently immobilizing capture antibodies on corresponding sensing sites on a silanized disposable glass chip. Gold nanoparticle-based bioconjugates with a high molar ratio of horseradish peroxidase (HRP) to detection antibodies were used for signal amplification. Under a sandwich immunoassay, the CL signals triggered by HRP captured on each sensing cell were collected by a charge-coupled device for simultaneous measurement of biomarkers and combination diagnosis of certain tumors. As a proof of concept, the immunosensor array was applied to detect α-fetoprotein, carcinoma antigen 125, carbohydrate antigen 153, and carcinoembryonic antigen and to screen patients with liver, breast, or ovarian cancers. This method showed wide linear ranges over 5 orders of magnitude and much lower detection limits than previously reported multiplexed immunoassays. The high throughput and acceptable stability, reproducibility, and accuracy showed good applicability of the proposed multiplex CL imaging immunoassay in clinical diagnosis. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/22320247/Chemiluminescence_imaging_immunoassay_of_multiple_tumor_markers_for_cancer_screening_ L2 - https://dx.doi.org/10.1021/ac203179g DB - PRIME DP - Unbound Medicine ER -