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Secreted protein acidic and rich in cysteine (SPARC) is associated with nasopharyngeal carcinoma metastasis and poor prognosis.
J Transl Med 2012; 10:27JT

Abstract

BACKGROUND

The aim of the present study was to analyse the expression of Secreted protein acidic and rich in cysteine (SPARC) in nasopharyngeal carcinoma (NPC) specimens, and to evaluate its correlation with clinicopathologic features, including survival of patients with NPC.

METHODS

NPC tissue microarrays (TMAs) were constructed from Sun Yat-sen University Cancer Center (SYSUCC), another three centers on mainland China, Singapore and Hong Kong. Using quantitative RT-PCR and Western-blotting techniques, we detected mRNA and protein expression of SPARC in NPC cell lines and immortalized nasopharyngeal epithelial cells (NPECs) induced by Bmi-1 (NPEC2 Bmi-1). The difference of SPARC expression in the cell lines was tested using a t-test method. The relationship between the SPARC expression and clinicopathological data was assessed by chi-square. Survival analysis was estimated using the Kaplan-Meier approach with log-rank test. Univariate and multivariate analyses of clinical variables were performed using Cox proportional hazards regression models.

RESULTS

The expression levels of SPARC mRNA and protein were markedly higher in NPC cell lines than in NPEC2 Bmi-1. Especially, the expression levels of SPARC mRNA and protein were much lower in the 6-10B than in the 5-8 F (P = 0.002, P = 0.001). SPARC immunostaining revealed cytoplasmic localization in NPC cells and no staining in the stroma and epithelium. In addition, high level of SPARC positively correlated with the status of distant metastasis (P = 0.001) and WHO histological classification (P = 0.023). NPC patients with high SPARC expression also had a significantly poorer prognosis than patients with low SPARC expression (log-rank test, P < 0.001), especially patients with advanced stage disease (log-rank, P < 0.001). Multivariate analysis suggested that the level of SPARC expression was an independent prognostic indicator for the overall survival of patients with NPC (P < 0.001).

CONCLUSIONS

SPARC expression is common in NPC patients. Our data shows that elevated SPARC expression is a potential unfavorable prognostic factor for patients with NPC.

Authors+Show Affiliations

State Key Laboratory of Oncology in Southern China, Sun Yat-Sen University Cancer Center, Guangzhou 510060, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22321704

Citation

Wang, Hai-Yun, et al. "Secreted Protein Acidic and Rich in Cysteine (SPARC) Is Associated With Nasopharyngeal Carcinoma Metastasis and Poor Prognosis." Journal of Translational Medicine, vol. 10, 2012, p. 27.
Wang HY, Li YY, Shao Q, et al. Secreted protein acidic and rich in cysteine (SPARC) is associated with nasopharyngeal carcinoma metastasis and poor prognosis. J Transl Med. 2012;10:27.
Wang, H. Y., Li, Y. Y., Shao, Q., Hou, J. H., Wang, F., Cai, M. B., ... Shao, J. Y. (2012). Secreted protein acidic and rich in cysteine (SPARC) is associated with nasopharyngeal carcinoma metastasis and poor prognosis. Journal of Translational Medicine, 10, p. 27. doi:10.1186/1479-5876-10-27.
Wang HY, et al. Secreted Protein Acidic and Rich in Cysteine (SPARC) Is Associated With Nasopharyngeal Carcinoma Metastasis and Poor Prognosis. J Transl Med. 2012 Feb 9;10:27. PubMed PMID: 22321704.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Secreted protein acidic and rich in cysteine (SPARC) is associated with nasopharyngeal carcinoma metastasis and poor prognosis. AU - Wang,Hai-Yun, AU - Li,Yang-Yang, AU - Shao,Qiong, AU - Hou,Jing-Hui, AU - Wang,Fang, AU - Cai,Man-Bo, AU - Zeng,Yi-Xin, AU - Shao,Jian-Yong, Y1 - 2012/02/09/ PY - 2011/08/22/received PY - 2012/02/09/accepted PY - 2012/2/11/entrez PY - 2012/2/11/pubmed PY - 2012/5/19/medline SP - 27 EP - 27 JF - Journal of translational medicine JO - J Transl Med VL - 10 N2 - BACKGROUND: The aim of the present study was to analyse the expression of Secreted protein acidic and rich in cysteine (SPARC) in nasopharyngeal carcinoma (NPC) specimens, and to evaluate its correlation with clinicopathologic features, including survival of patients with NPC. METHODS: NPC tissue microarrays (TMAs) were constructed from Sun Yat-sen University Cancer Center (SYSUCC), another three centers on mainland China, Singapore and Hong Kong. Using quantitative RT-PCR and Western-blotting techniques, we detected mRNA and protein expression of SPARC in NPC cell lines and immortalized nasopharyngeal epithelial cells (NPECs) induced by Bmi-1 (NPEC2 Bmi-1). The difference of SPARC expression in the cell lines was tested using a t-test method. The relationship between the SPARC expression and clinicopathological data was assessed by chi-square. Survival analysis was estimated using the Kaplan-Meier approach with log-rank test. Univariate and multivariate analyses of clinical variables were performed using Cox proportional hazards regression models. RESULTS: The expression levels of SPARC mRNA and protein were markedly higher in NPC cell lines than in NPEC2 Bmi-1. Especially, the expression levels of SPARC mRNA and protein were much lower in the 6-10B than in the 5-8 F (P = 0.002, P = 0.001). SPARC immunostaining revealed cytoplasmic localization in NPC cells and no staining in the stroma and epithelium. In addition, high level of SPARC positively correlated with the status of distant metastasis (P = 0.001) and WHO histological classification (P = 0.023). NPC patients with high SPARC expression also had a significantly poorer prognosis than patients with low SPARC expression (log-rank test, P < 0.001), especially patients with advanced stage disease (log-rank, P < 0.001). Multivariate analysis suggested that the level of SPARC expression was an independent prognostic indicator for the overall survival of patients with NPC (P < 0.001). CONCLUSIONS: SPARC expression is common in NPC patients. Our data shows that elevated SPARC expression is a potential unfavorable prognostic factor for patients with NPC. SN - 1479-5876 UR - https://www.unboundmedicine.com/medline/citation/22321704/Secreted_protein_acidic_and_rich_in_cysteine__SPARC__is_associated_with_nasopharyngeal_carcinoma_metastasis_and_poor_prognosis_ L2 - https://translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-10-27 DB - PRIME DP - Unbound Medicine ER -