Tags

Type your tag names separated by a space and hit enter

[Interaction of fatty acid oxidation with oxidative stress in preeclampsia-like mouse model at multiple stages of gestation].
Zhonghua Yi Xue Za Zhi. 2011 Sep 06; 91(33):2343-7.ZY

Abstract

OBJECTIVE

To investigate the interactions of fatty acid oxidation with oxidative stress on the preeclampsia (PE)-like development by establishing murine models at multiple stages of gestation.

METHODS

Wild-type (WT) mice were injected subcutaneously with nitric oxide synthase (NOS) inhibitor L-arginine methyl ester (L-NAME, 50 mg×kg(-1)×d(-1)) to establish PE-like model (L-NAME group) at early, middle and late pregnant periods respectively. Normal saline (NS group) was injected simultaneously as a control. All groups were divided into subgroups, standard chow group (SC) and high-fat diet group (HF). From Day 1 of pregnancy, all groups were fed simultaneously. ApoE(-/-) pregnant mice were selected as a control group. Identification of mice model and the expressions of mRNA and protein of LCHAD, p47phox, p38MAPK and COX-2 were confirmed by RT-PCR (reverse transcription-polymerase chain reaction) and Western blot respectively. Data were analyzed statistically.

RESULTS

The expressions of mRNA and protein of LCHAD significantly decreased in early and middle L-NAME groups in both apoE(-/-) and WT mice (P < 0.05). The expressions of mRNA and protein of LCHAD in the HF groups were lower than the SC groups. The changes were marked in the early HF + L-NAME subgroup in apoE(-/-) mice (P < 0.05). As compared with other groups, the placental expressions of mRNA and protein of p47phox and COX-2 markedly increased in the early and middle L-NAME subgroups in apoE(-/-) mice (P < 0.05), especially in early and middle HF + L-NAME subgroups in apoE(-/-) mice (P < 0.05). ApoE(-/-) mice had a higher expression than that of WT groups (P < 0.05). The expressions of mRNA and protein of p38MAPK showed no differences among all groups. Correlation analysis showed a negative correlation between gene and protein expressions of LCHAD and p47phox, COX-2 (P < 0.05). There was significantly positively correlated between the expression of p47phox, COX-2 and protein (P < 0.05).

CONCLUSIONS

The PE-like symptoms have fatty acid oxidation dysfunctions. There is a lower expression of LCHAD. The earlier its onset, the more obvious dysfunctions. In early and middle L-NAME groups, oxidative stress and inflammatory injury occur in both apoE(-/-) and WT mice. A high-fat diet may aggravate the level of oxidative stress especially in PE-like apoE(-/-) murine model.

Authors+Show Affiliations

Department of Obstetrics & Gynecology, Peking University Third Hospital, Beijing 100191, China.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

chi

PubMed ID

22321750

Citation

Sun, Min-Na, et al. "[Interaction of Fatty Acid Oxidation With Oxidative Stress in Preeclampsia-like Mouse Model at Multiple Stages of Gestation]." Zhonghua Yi Xue Za Zhi, vol. 91, no. 33, 2011, pp. 2343-7.
Sun MN, Yang Z, Ma RQ. [Interaction of fatty acid oxidation with oxidative stress in preeclampsia-like mouse model at multiple stages of gestation]. Zhonghua Yi Xue Za Zhi. 2011;91(33):2343-7.
Sun, M. N., Yang, Z., & Ma, R. Q. (2011). [Interaction of fatty acid oxidation with oxidative stress in preeclampsia-like mouse model at multiple stages of gestation]. Zhonghua Yi Xue Za Zhi, 91(33), 2343-7.
Sun MN, Yang Z, Ma RQ. [Interaction of Fatty Acid Oxidation With Oxidative Stress in Preeclampsia-like Mouse Model at Multiple Stages of Gestation]. Zhonghua Yi Xue Za Zhi. 2011 Sep 6;91(33):2343-7. PubMed PMID: 22321750.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Interaction of fatty acid oxidation with oxidative stress in preeclampsia-like mouse model at multiple stages of gestation]. AU - Sun,Min-Na, AU - Yang,Zi, AU - Ma,Rui-Qiong, PY - 2012/2/11/entrez PY - 2012/2/11/pubmed PY - 2016/4/24/medline SP - 2343 EP - 7 JF - Zhonghua yi xue za zhi JO - Zhonghua Yi Xue Za Zhi VL - 91 IS - 33 N2 - OBJECTIVE: To investigate the interactions of fatty acid oxidation with oxidative stress on the preeclampsia (PE)-like development by establishing murine models at multiple stages of gestation. METHODS: Wild-type (WT) mice were injected subcutaneously with nitric oxide synthase (NOS) inhibitor L-arginine methyl ester (L-NAME, 50 mg×kg(-1)×d(-1)) to establish PE-like model (L-NAME group) at early, middle and late pregnant periods respectively. Normal saline (NS group) was injected simultaneously as a control. All groups were divided into subgroups, standard chow group (SC) and high-fat diet group (HF). From Day 1 of pregnancy, all groups were fed simultaneously. ApoE(-/-) pregnant mice were selected as a control group. Identification of mice model and the expressions of mRNA and protein of LCHAD, p47phox, p38MAPK and COX-2 were confirmed by RT-PCR (reverse transcription-polymerase chain reaction) and Western blot respectively. Data were analyzed statistically. RESULTS: The expressions of mRNA and protein of LCHAD significantly decreased in early and middle L-NAME groups in both apoE(-/-) and WT mice (P < 0.05). The expressions of mRNA and protein of LCHAD in the HF groups were lower than the SC groups. The changes were marked in the early HF + L-NAME subgroup in apoE(-/-) mice (P < 0.05). As compared with other groups, the placental expressions of mRNA and protein of p47phox and COX-2 markedly increased in the early and middle L-NAME subgroups in apoE(-/-) mice (P < 0.05), especially in early and middle HF + L-NAME subgroups in apoE(-/-) mice (P < 0.05). ApoE(-/-) mice had a higher expression than that of WT groups (P < 0.05). The expressions of mRNA and protein of p38MAPK showed no differences among all groups. Correlation analysis showed a negative correlation between gene and protein expressions of LCHAD and p47phox, COX-2 (P < 0.05). There was significantly positively correlated between the expression of p47phox, COX-2 and protein (P < 0.05). CONCLUSIONS: The PE-like symptoms have fatty acid oxidation dysfunctions. There is a lower expression of LCHAD. The earlier its onset, the more obvious dysfunctions. In early and middle L-NAME groups, oxidative stress and inflammatory injury occur in both apoE(-/-) and WT mice. A high-fat diet may aggravate the level of oxidative stress especially in PE-like apoE(-/-) murine model. SN - 0376-2491 UR - https://www.unboundmedicine.com/medline/citation/22321750/[Interaction_of_fatty_acid_oxidation_with_oxidative_stress_in_preeclampsia_like_mouse_model_at_multiple_stages_of_gestation]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&amp;issn=0376-2491&amp;year=2011&amp;vol=91&amp;issue=33&amp;fpage=2343 DB - PRIME DP - Unbound Medicine ER -