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DNA alterations of microsatellite DNA, p53, APC and K-ras in Chinese colorectal cancer patients.
Eur J Clin Invest. 2012 Jul; 42(7):751-9.EJ

Abstract

BACKGROUND

Colorectal cancer is one of the most rapidly increasing cancers in the world, and accumulation of alterations in oncogenes, tumour suppressor genes and mismatch repair (MMR) genes contributes to colorectal tumorigenesis. Thus, we investigated the alterations of 14 microsatellite loci adjacent to MMR genes, p53, adenomatous polyposis coli (APC) and K-ras in 52 Chinese patients with colorectal cancer.

MATERIALS AND METHODS

We performed fluorescent polymerase chain reaction and capillary electrophoresis to analyse microsatellite instability (MSI) and loss of heterozygosity (LOH) in microsatellite loci, which included a panel of nine dinucleotide repeats and the Bethesda consensus panel. Additionally, we screened for mutations in exons 4-9 of p53 and the mutation cluster region (MCR) in APC by DHPLC. Codons 12, 13 and 61 in K-ras were analysed using direct sequencing. All variations were confirmed using clone sequencing.

RESULTS

The alteration frequency of microsatellite DNA was 55·8% (29/52). Among the microsatellites, five loci exhibited MSI and another nine loci exhibited LOH. The mutation rates of p53, APC and K-ras were 42·3%, 38·5% and 36·5%, respectively. All patients (n = 7) with liver metastasis had a mutation in p53, APC or K-ras. APC mutation was correlated with clinical stage and the presence of lymph node metastasis (P = 0·001 and P = 0·006, respectively). CONCLUSIONS> A total of 80·8% of Chinese patients with colorectal cancer show variations in microsatellite DNA, p53, APC or K-ras. It appears that these microsatellite DNA alterations could be a new biomarker for colorectal cancer.

Authors+Show Affiliations

Graduate School, Chinese Academy of Medical Sciences & Peking Union Medical College, Dongdan, Dongcheng District, Beijing, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22324744

Citation

Xu, Xin-Min, et al. "DNA Alterations of Microsatellite DNA, P53, APC and K-ras in Chinese Colorectal Cancer Patients." European Journal of Clinical Investigation, vol. 42, no. 7, 2012, pp. 751-9.
Xu XM, Qian JC, Cai Z, et al. DNA alterations of microsatellite DNA, p53, APC and K-ras in Chinese colorectal cancer patients. Eur J Clin Invest. 2012;42(7):751-9.
Xu, X. M., Qian, J. C., Cai, Z., Tang, T., Wang, P., Zhang, K. H., Deng, Z. L., & Cai, J. P. (2012). DNA alterations of microsatellite DNA, p53, APC and K-ras in Chinese colorectal cancer patients. European Journal of Clinical Investigation, 42(7), 751-9. https://doi.org/10.1111/j.1365-2362.2011.02641.x
Xu XM, et al. DNA Alterations of Microsatellite DNA, P53, APC and K-ras in Chinese Colorectal Cancer Patients. Eur J Clin Invest. 2012;42(7):751-9. PubMed PMID: 22324744.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - DNA alterations of microsatellite DNA, p53, APC and K-ras in Chinese colorectal cancer patients. AU - Xu,Xin-Min, AU - Qian,Jian-Chang, AU - Cai,Zhe, AU - Tang,Tao, AU - Wang,Peng, AU - Zhang,Ke-Hua, AU - Deng,Zhou-Lu, AU - Cai,Jian-Ping, Y1 - 2012/02/11/ PY - 2012/2/14/entrez PY - 2012/2/14/pubmed PY - 2012/12/10/medline SP - 751 EP - 9 JF - European journal of clinical investigation JO - Eur. J. Clin. Invest. VL - 42 IS - 7 N2 - BACKGROUND: Colorectal cancer is one of the most rapidly increasing cancers in the world, and accumulation of alterations in oncogenes, tumour suppressor genes and mismatch repair (MMR) genes contributes to colorectal tumorigenesis. Thus, we investigated the alterations of 14 microsatellite loci adjacent to MMR genes, p53, adenomatous polyposis coli (APC) and K-ras in 52 Chinese patients with colorectal cancer. MATERIALS AND METHODS: We performed fluorescent polymerase chain reaction and capillary electrophoresis to analyse microsatellite instability (MSI) and loss of heterozygosity (LOH) in microsatellite loci, which included a panel of nine dinucleotide repeats and the Bethesda consensus panel. Additionally, we screened for mutations in exons 4-9 of p53 and the mutation cluster region (MCR) in APC by DHPLC. Codons 12, 13 and 61 in K-ras were analysed using direct sequencing. All variations were confirmed using clone sequencing. RESULTS: The alteration frequency of microsatellite DNA was 55·8% (29/52). Among the microsatellites, five loci exhibited MSI and another nine loci exhibited LOH. The mutation rates of p53, APC and K-ras were 42·3%, 38·5% and 36·5%, respectively. All patients (n = 7) with liver metastasis had a mutation in p53, APC or K-ras. APC mutation was correlated with clinical stage and the presence of lymph node metastasis (P = 0·001 and P = 0·006, respectively). CONCLUSIONS> A total of 80·8% of Chinese patients with colorectal cancer show variations in microsatellite DNA, p53, APC or K-ras. It appears that these microsatellite DNA alterations could be a new biomarker for colorectal cancer. SN - 1365-2362 UR - https://www.unboundmedicine.com/medline/citation/22324744/DNA_alterations_of_microsatellite_DNA_p53_APC_and_K_ras_in_Chinese_colorectal_cancer_patients_ L2 - https://doi.org/10.1111/j.1365-2362.2011.02641.x DB - PRIME DP - Unbound Medicine ER -