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Alternation of immune parameters and cellular energy allocation of Chlamys farreri under ammonia-N exposure and Vibrio anguillarum challenge.
Fish Shellfish Immunol. 2012 May; 32(5):741-9.FS

Abstract

The complex interactions among host, pathogen and environment are believed to be the main causes for the mass mortality of cultured scallops. In the present study, the temporal variations of immune parameters and cellular energy allocation (CEA) of Chlamys farreri under ammonia-N, Vibrio anguillarum as well as their combined treatment were investigated to better understand the energetic mechanisms of scallop in immune defense. After 1 d exposure to ammonia-N, V. anguillarum and their combination, the superoxide anion level and superoxide dismutase (SOD) activity in the serum of scallops increased substantially. At 24 d post exposure, the mRNA expression levels of isocitrate dehydrogenase (IDH), heat shock protein 70 (HSP 70), HSP 90 and glutamine synthetase (GS), as well as the malondialdehyde content remarkably increased, while SOD activity was depressed significantly (P < 0.05). The glycogen reserved in the tissues from scallops exposed to the combined stress for 1 d, 12 d and 24 d were significantly lower than those in the control (P < 0.05). The CEA values in all the examined tissues including gonad, gill, hepatopancreas and adductor muscle were significantly lower than those of control (P < 0.05) when exposure to ammonia-N, V. anguillarum and their combined treatment for 12 and 24 d. Furthermore, the combined stress also had a significant impact upon CEA in all the examined tissues in scallops post 1 d exposure (P < 0.05). The above results demonstrated that SOD, IDH, HSPs and GS in hemolymph of treated scallops are necessary, but not sufficient to the complete protection against stress-induced cellular damage along with the treatment duration. Immune defense against the combination of pathogen invasion and environmental stress can impose greater costs on scallop's energy expenditure than a single stressor, and the combined treatment preferentially consumed more available glycogen in scallops for immune defense. Hence, in addition to be used in immunological evaluation, CEA is also a powerful tool to provide valuable insights into possible mechanisms of mass mortalities in cultured scallops.

Authors+Show Affiliations

Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22326939

Citation

Wang, Xingqiang, et al. "Alternation of Immune Parameters and Cellular Energy Allocation of Chlamys Farreri Under ammonia-N Exposure and Vibrio Anguillarum Challenge." Fish & Shellfish Immunology, vol. 32, no. 5, 2012, pp. 741-9.
Wang X, Wang L, Yao C, et al. Alternation of immune parameters and cellular energy allocation of Chlamys farreri under ammonia-N exposure and Vibrio anguillarum challenge. Fish Shellfish Immunol. 2012;32(5):741-9.
Wang, X., Wang, L., Yao, C., Qiu, L., Zhang, H., Zhi, Z., & Song, L. (2012). Alternation of immune parameters and cellular energy allocation of Chlamys farreri under ammonia-N exposure and Vibrio anguillarum challenge. Fish & Shellfish Immunology, 32(5), 741-9. https://doi.org/10.1016/j.fsi.2012.01.025
Wang X, et al. Alternation of Immune Parameters and Cellular Energy Allocation of Chlamys Farreri Under ammonia-N Exposure and Vibrio Anguillarum Challenge. Fish Shellfish Immunol. 2012;32(5):741-9. PubMed PMID: 22326939.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Alternation of immune parameters and cellular energy allocation of Chlamys farreri under ammonia-N exposure and Vibrio anguillarum challenge. AU - Wang,Xingqiang, AU - Wang,Lingling, AU - Yao,Chen, AU - Qiu,Limei, AU - Zhang,Huan, AU - Zhi,Zhou, AU - Song,Linsheng, Y1 - 2012/02/03/ PY - 2011/11/24/received PY - 2012/01/20/revised PY - 2012/01/29/accepted PY - 2012/2/14/entrez PY - 2012/2/14/pubmed PY - 2012/7/28/medline SP - 741 EP - 9 JF - Fish & shellfish immunology JO - Fish Shellfish Immunol. VL - 32 IS - 5 N2 - The complex interactions among host, pathogen and environment are believed to be the main causes for the mass mortality of cultured scallops. In the present study, the temporal variations of immune parameters and cellular energy allocation (CEA) of Chlamys farreri under ammonia-N, Vibrio anguillarum as well as their combined treatment were investigated to better understand the energetic mechanisms of scallop in immune defense. After 1 d exposure to ammonia-N, V. anguillarum and their combination, the superoxide anion level and superoxide dismutase (SOD) activity in the serum of scallops increased substantially. At 24 d post exposure, the mRNA expression levels of isocitrate dehydrogenase (IDH), heat shock protein 70 (HSP 70), HSP 90 and glutamine synthetase (GS), as well as the malondialdehyde content remarkably increased, while SOD activity was depressed significantly (P < 0.05). The glycogen reserved in the tissues from scallops exposed to the combined stress for 1 d, 12 d and 24 d were significantly lower than those in the control (P < 0.05). The CEA values in all the examined tissues including gonad, gill, hepatopancreas and adductor muscle were significantly lower than those of control (P < 0.05) when exposure to ammonia-N, V. anguillarum and their combined treatment for 12 and 24 d. Furthermore, the combined stress also had a significant impact upon CEA in all the examined tissues in scallops post 1 d exposure (P < 0.05). The above results demonstrated that SOD, IDH, HSPs and GS in hemolymph of treated scallops are necessary, but not sufficient to the complete protection against stress-induced cellular damage along with the treatment duration. Immune defense against the combination of pathogen invasion and environmental stress can impose greater costs on scallop's energy expenditure than a single stressor, and the combined treatment preferentially consumed more available glycogen in scallops for immune defense. Hence, in addition to be used in immunological evaluation, CEA is also a powerful tool to provide valuable insights into possible mechanisms of mass mortalities in cultured scallops. SN - 1095-9947 UR - https://www.unboundmedicine.com/medline/citation/22326939/Alternation_of_immune_parameters_and_cellular_energy_allocation_of_Chlamys_farreri_under_ammonia_N_exposure_and_Vibrio_anguillarum_challenge_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1050-4648(12)00036-8 DB - PRIME DP - Unbound Medicine ER -