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HMGB1-RAGE regulates muscle satellite cell homeostasis through p38-MAPK- and myogenin-dependent repression of Pax7 transcription.
J Cell Sci. 2012 Mar 15; 125(Pt 6):1440-54.JC

Abstract

Expression of the paired-box 7 (PAX7) transcription factor is regulated during both myoblast proliferation and differentiation: high levels of PAX7 compromise myogenic differentiation because of excess and prolonged proliferation, whereas low levels of PAX7 result in precocious differentiation. We showed that myogenin repressed Pax7 transcription in differentiating myoblasts by binding to specific recognition sites in the Pax7 promoter, and that high-mobility group box 1 (HMGB1)-receptor for advanced glycation end-products (RAGE) signaling was required for myogenin induction and myogenin-dependent repression of Pax7 transcription. In addition, PAX7 negatively and myogenin positively regulated RAGE expression. RAGE, a multiligand receptor of the immunoglobulin superfamily, was not expressed in adult skeletal muscles, and was transiently expressed in activated, proliferating and differentiating satellite cells (SCs) in injured muscles. Compared with wild-type muscles, Rage(-/-) muscles exhibited increased numbers of basal SCs that were further increased in injured Rage(-/-) muscles following elevated myoblast asymmetric division; complete regeneration of injured Rage(-/-) muscles was found to be delayed by ~1 week. Thus, RAGE signaling physiologically repressed Pax7 transcription in SCs by upregulating myogenin, thereby accelerating muscle regeneration and limiting SC self-renewal.

Authors+Show Affiliations

Department of Experimental Medicine and Biochemical Sciences and Istituto Interuniversitario di Miologia, University of Perugia, Via del Giochetto, 06122, Perugia, Italy.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22328527

Citation

Riuzzi, Francesca, et al. "HMGB1-RAGE Regulates Muscle Satellite Cell Homeostasis Through p38-MAPK- and Myogenin-dependent Repression of Pax7 Transcription." Journal of Cell Science, vol. 125, no. Pt 6, 2012, pp. 1440-54.
Riuzzi F, Sorci G, Sagheddu R, et al. HMGB1-RAGE regulates muscle satellite cell homeostasis through p38-MAPK- and myogenin-dependent repression of Pax7 transcription. J Cell Sci. 2012;125(Pt 6):1440-54.
Riuzzi, F., Sorci, G., Sagheddu, R., & Donato, R. (2012). HMGB1-RAGE regulates muscle satellite cell homeostasis through p38-MAPK- and myogenin-dependent repression of Pax7 transcription. Journal of Cell Science, 125(Pt 6), 1440-54. https://doi.org/10.1242/jcs.092163
Riuzzi F, et al. HMGB1-RAGE Regulates Muscle Satellite Cell Homeostasis Through p38-MAPK- and Myogenin-dependent Repression of Pax7 Transcription. J Cell Sci. 2012 Mar 15;125(Pt 6):1440-54. PubMed PMID: 22328527.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - HMGB1-RAGE regulates muscle satellite cell homeostasis through p38-MAPK- and myogenin-dependent repression of Pax7 transcription. AU - Riuzzi,Francesca, AU - Sorci,Guglielmo, AU - Sagheddu,Roberta, AU - Donato,Rosario, Y1 - 2012/02/10/ PY - 2012/2/14/entrez PY - 2012/2/14/pubmed PY - 2013/2/12/medline SP - 1440 EP - 54 JF - Journal of cell science JO - J Cell Sci VL - 125 IS - Pt 6 N2 - Expression of the paired-box 7 (PAX7) transcription factor is regulated during both myoblast proliferation and differentiation: high levels of PAX7 compromise myogenic differentiation because of excess and prolonged proliferation, whereas low levels of PAX7 result in precocious differentiation. We showed that myogenin repressed Pax7 transcription in differentiating myoblasts by binding to specific recognition sites in the Pax7 promoter, and that high-mobility group box 1 (HMGB1)-receptor for advanced glycation end-products (RAGE) signaling was required for myogenin induction and myogenin-dependent repression of Pax7 transcription. In addition, PAX7 negatively and myogenin positively regulated RAGE expression. RAGE, a multiligand receptor of the immunoglobulin superfamily, was not expressed in adult skeletal muscles, and was transiently expressed in activated, proliferating and differentiating satellite cells (SCs) in injured muscles. Compared with wild-type muscles, Rage(-/-) muscles exhibited increased numbers of basal SCs that were further increased in injured Rage(-/-) muscles following elevated myoblast asymmetric division; complete regeneration of injured Rage(-/-) muscles was found to be delayed by ~1 week. Thus, RAGE signaling physiologically repressed Pax7 transcription in SCs by upregulating myogenin, thereby accelerating muscle regeneration and limiting SC self-renewal. SN - 1477-9137 UR - https://www.unboundmedicine.com/medline/citation/22328527/HMGB1_RAGE_regulates_muscle_satellite_cell_homeostasis_through_p38_MAPK__and_myogenin_dependent_repression_of_Pax7_transcription_ L2 - http://jcs.biologists.org/cgi/pmidlookup?view=long&pmid=22328527 DB - PRIME DP - Unbound Medicine ER -