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A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis.
Mol Cell Biol 2012; 32(8):1396-407MC

Abstract

Cytokinesis is a crucial step in the creation of two daughter cells by the formation and ingression of the cleavage furrow. Here, we show that sphingomyelin (SM), one of the major sphingolipids in mammalian cells, is required for the localization of phosphatidylinositol-4,5-bisphosphate (PIP(2)) to the cleavage furrow during cytokinesis. Real-time observation with a labeled SM-specific protein, lysenin, revealed that SM is concentrated in the outer leaflet of the furrow at the time of cytokinesis. Superresolution fluorescence microscopy analysis indicates a transbilayer colocalization between the SM-rich domains in the outer leaflet and PIP(2)-rich domains in the inner leaflet of the plasma membrane. The depletion of SM disperses PIP(2) and inhibits the recruitment of the small GTPase RhoA to the cleavage furrow, leading to abnormal cytokinesis. These results suggest that the formation of SM-rich domains is required for the accumulation of PIP(2) to the cleavage furrow, which is a prerequisite for the proper translocation of RhoA and the progression of cytokinesis.

Authors+Show Affiliations

Lipid Biology Laboratory, RIKEN Advanced Science Institute, Wako, Saitama, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22331463

Citation

Abe, Mitsuhiro, et al. "A Role for Sphingomyelin-rich Lipid Domains in the Accumulation of Phosphatidylinositol-4,5-bisphosphate to the Cleavage Furrow During Cytokinesis." Molecular and Cellular Biology, vol. 32, no. 8, 2012, pp. 1396-407.
Abe M, Makino A, Hullin-Matsuda F, et al. A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol Cell Biol. 2012;32(8):1396-407.
Abe, M., Makino, A., Hullin-Matsuda, F., Kamijo, K., Ohno-Iwashita, Y., Hanada, K., ... Kobayashi, T. (2012). A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Molecular and Cellular Biology, 32(8), pp. 1396-407. doi:10.1128/MCB.06113-11.
Abe M, et al. A Role for Sphingomyelin-rich Lipid Domains in the Accumulation of Phosphatidylinositol-4,5-bisphosphate to the Cleavage Furrow During Cytokinesis. Mol Cell Biol. 2012;32(8):1396-407. PubMed PMID: 22331463.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. AU - Abe,Mitsuhiro, AU - Makino,Asami, AU - Hullin-Matsuda,Françoise, AU - Kamijo,Keiju, AU - Ohno-Iwashita,Yoshiko, AU - Hanada,Kentaro, AU - Mizuno,Hideaki, AU - Miyawaki,Atsushi, AU - Kobayashi,Toshihide, Y1 - 2012/02/13/ PY - 2012/2/15/entrez PY - 2012/2/15/pubmed PY - 2012/6/30/medline SP - 1396 EP - 407 JF - Molecular and cellular biology JO - Mol. Cell. Biol. VL - 32 IS - 8 N2 - Cytokinesis is a crucial step in the creation of two daughter cells by the formation and ingression of the cleavage furrow. Here, we show that sphingomyelin (SM), one of the major sphingolipids in mammalian cells, is required for the localization of phosphatidylinositol-4,5-bisphosphate (PIP(2)) to the cleavage furrow during cytokinesis. Real-time observation with a labeled SM-specific protein, lysenin, revealed that SM is concentrated in the outer leaflet of the furrow at the time of cytokinesis. Superresolution fluorescence microscopy analysis indicates a transbilayer colocalization between the SM-rich domains in the outer leaflet and PIP(2)-rich domains in the inner leaflet of the plasma membrane. The depletion of SM disperses PIP(2) and inhibits the recruitment of the small GTPase RhoA to the cleavage furrow, leading to abnormal cytokinesis. These results suggest that the formation of SM-rich domains is required for the accumulation of PIP(2) to the cleavage furrow, which is a prerequisite for the proper translocation of RhoA and the progression of cytokinesis. SN - 1098-5549 UR - https://www.unboundmedicine.com/medline/citation/22331463/A_role_for_sphingomyelin_rich_lipid_domains_in_the_accumulation_of_phosphatidylinositol_45_bisphosphate_to_the_cleavage_furrow_during_cytokinesis_ L2 - http://mcb.asm.org/cgi/pmidlookup?view=long&pmid=22331463 DB - PRIME DP - Unbound Medicine ER -