Tags

Type your tag names separated by a space and hit enter

Bioactive stilbenes from a Bacillus sp. N strain associated with a novel rhabditid entomopathogenic nematode.
Lett Appl Microbiol. 2012 May; 54(5):410-7.LA

Abstract

AIMS

The aim of the present study was to purify and characterize a natural antimicrobial compound from Bacillus sp. strain N associated with a novel rhabditid entomopathogenic nematode.

METHODS AND RESULTS

The cell-free culture filtrate of a bacterium associated with a novel entomopathogenic nematode (EPN), Rhabditis (Oscheius) sp. exhibited strong antimicrobial activity. The ethyl acetate extract of the bacterial culture filtrate was purified by column chromatography, and two bioactive compounds were isolated and their chemical structures were established based on spectral analysis. The compounds were identified as 3,4',5-trihydroxystilbene (1) and 3,5-dihydroxy-4-isopropylstilbene (2). The presence of 3,4',5-trihydroxystilbene (resveratrol) is reported for the first time in bacteria. Compound 1 showed antibacterial activity against all the four test bacteria, whereas compound 2 was effective against the Gram-positive bacteria only. Compounds 1 and 2 were active against all the five fungi tested and are more effective than bavistin, the standard fungicide. The antifungal activity of the compounds against the plant pathogenic fungi, Rhizoctonia solani is reported for the first time.

CONCLUSIONS

Cell-free extract of the bacterium and isolated stilbenes demonstrated high antibacterial activity against bacteria and fungi especially against plant pathogenic fungi. We conclude that the bacterium-associated EPN are promising sources of natural bioactive secondary metabolites.

SIGNIFICANCE AND IMPACT OF THE STUDY

Stilbene compounds can be used for the control of fungi and bacteria.

Authors+Show Affiliations

Division of Crop Protection/Division of Crop Utilisation, Central Tuber Crops Research Institute, Thiruvananthapuram, Kerala, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22332977

Citation

Kumar, S N., et al. "Bioactive Stilbenes From a Bacillus Sp. N Strain Associated With a Novel Rhabditid Entomopathogenic Nematode." Letters in Applied Microbiology, vol. 54, no. 5, 2012, pp. 410-7.
Kumar SN, Siji JV, Rajasekharan KN, et al. Bioactive stilbenes from a Bacillus sp. N strain associated with a novel rhabditid entomopathogenic nematode. Lett Appl Microbiol. 2012;54(5):410-7.
Kumar, S. N., Siji, J. V., Rajasekharan, K. N., Nambisan, B., & Mohandas, C. (2012). Bioactive stilbenes from a Bacillus sp. N strain associated with a novel rhabditid entomopathogenic nematode. Letters in Applied Microbiology, 54(5), 410-7. https://doi.org/10.1111/j.1472-765X.2012.03223.x
Kumar SN, et al. Bioactive Stilbenes From a Bacillus Sp. N Strain Associated With a Novel Rhabditid Entomopathogenic Nematode. Lett Appl Microbiol. 2012;54(5):410-7. PubMed PMID: 22332977.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bioactive stilbenes from a Bacillus sp. N strain associated with a novel rhabditid entomopathogenic nematode. AU - Kumar,S N, AU - Siji,J V, AU - Rajasekharan,K N, AU - Nambisan,B, AU - Mohandas,C, Y1 - 2012/03/06/ PY - 2012/2/16/entrez PY - 2012/2/16/pubmed PY - 2012/10/23/medline SP - 410 EP - 7 JF - Letters in applied microbiology JO - Lett Appl Microbiol VL - 54 IS - 5 N2 - AIMS: The aim of the present study was to purify and characterize a natural antimicrobial compound from Bacillus sp. strain N associated with a novel rhabditid entomopathogenic nematode. METHODS AND RESULTS: The cell-free culture filtrate of a bacterium associated with a novel entomopathogenic nematode (EPN), Rhabditis (Oscheius) sp. exhibited strong antimicrobial activity. The ethyl acetate extract of the bacterial culture filtrate was purified by column chromatography, and two bioactive compounds were isolated and their chemical structures were established based on spectral analysis. The compounds were identified as 3,4',5-trihydroxystilbene (1) and 3,5-dihydroxy-4-isopropylstilbene (2). The presence of 3,4',5-trihydroxystilbene (resveratrol) is reported for the first time in bacteria. Compound 1 showed antibacterial activity against all the four test bacteria, whereas compound 2 was effective against the Gram-positive bacteria only. Compounds 1 and 2 were active against all the five fungi tested and are more effective than bavistin, the standard fungicide. The antifungal activity of the compounds against the plant pathogenic fungi, Rhizoctonia solani is reported for the first time. CONCLUSIONS: Cell-free extract of the bacterium and isolated stilbenes demonstrated high antibacterial activity against bacteria and fungi especially against plant pathogenic fungi. We conclude that the bacterium-associated EPN are promising sources of natural bioactive secondary metabolites. SIGNIFICANCE AND IMPACT OF THE STUDY: Stilbene compounds can be used for the control of fungi and bacteria. SN - 1472-765X UR - https://www.unboundmedicine.com/medline/citation/22332977/Bioactive_stilbenes_from_a_Bacillus_sp__N_strain_associated_with_a_novel_rhabditid_entomopathogenic_nematode_ L2 - https://doi.org/10.1111/j.1472-765X.2012.03223.x DB - PRIME DP - Unbound Medicine ER -