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[Levels of HMGB1 in induced sputum from patients with asthma and chronic obstructive pulmonary disease].
Zhonghua Yi Xue Za Zhi. 2011 Nov 15; 91(42):2981-4.ZY

Abstract

OBJECTIVE

To explore the relationship between the sputum levels of high mobility group protein B1 (HMGB1) and airway inflammation in bronchial asthma and chronic obstructive pulmonary disease (COPD) patients.

METHODS

A total of 57 patients with persistent asthma [per Global Initiative for Asthma (GINA) guidelines], 30 patients with stable COPD [stratified by Global Initiative for COPD (GOLD) status] and 20 control subjects were recruited. After completing an asthma control questionnaire, spirometry was performed before sputum induction. The ratio of forced expiratory volume in the first second (FEV(1))/predictive value (FEV(1)%Pre) and neutrophil differential count in induced sputum were recorded. The concentrations of HMGB1 in the supernatant of sputum were measured by ELISA (enzyme-linked immunosorbent assay).

RESULTS

The sputum concentrations of HMGB1 in the asthmatics and COPD patients were significantly higher than those of the control subjects [(291 ± 55) and (511 ± 39) vs (61 ± 5) ng/L, all P < 0.01]. And they were significantly negatively correlated with FEV(1)%Pre in all subjects. The levels of HMGB1 in induced sputum of COPD patients were significantly higher than those of asthmatics and healthy controls (P < 0.01). No significant difference existed in the levels of HMGB1 between patients with eosinophilic and noneosinophilic asthma [(290 ± 55) vs (292 ± 54) ng/L, P > 0.05]. The HMGB1 levels with COPD stage II and stage III were significantly higher than those with stage I [(526 ± 29) and (541 ± 29) vs (471 ± 18) ng/L]. The differences of sputum neutrophil percentage were statistically significant in mild, moderate and severe asthma [(27 ± 2)%, (36 ± 4)%, (49 ± 4)%]. And the sputum levels of HMGB1 were significantly higher in the patients with moderate and severe asthma [(312 ± 14) vs (347 ± 11) ng/L]. And the levels of HMGB1 in asthmatic and COPD patients were positively correlated with neutrophil percentage. According to the multivariate analysis, neutrophil percentage and FEV(1)%Pre were independent predictors of sputum HMGB1, but not smoking, age, gender and eosinophilic percentage.

CONCLUSION

HMGB1 may contribute to airway inflammation through its higher expression in bronchial asthma and COPD patients.

Authors+Show Affiliations

Department of Respiratory and Critical Care Medicine, First Affiliated Hospital, Zhengzhou University, China. chengzhehi@126.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

22333024

Citation

Cheng, Zhe, et al. "[Levels of HMGB1 in Induced Sputum From Patients With Asthma and Chronic Obstructive Pulmonary Disease]." Zhonghua Yi Xue Za Zhi, vol. 91, no. 42, 2011, pp. 2981-4.
Cheng Z, Kang Y, Wu QG, et al. [Levels of HMGB1 in induced sputum from patients with asthma and chronic obstructive pulmonary disease]. Zhonghua Yi Xue Za Zhi. 2011;91(42):2981-4.
Cheng, Z., Kang, Y., Wu, Q. G., Dai, L. L., Song, Y. N., Xia, J., Si, J. M., & Chen, C. Y. (2011). [Levels of HMGB1 in induced sputum from patients with asthma and chronic obstructive pulmonary disease]. Zhonghua Yi Xue Za Zhi, 91(42), 2981-4.
Cheng Z, et al. [Levels of HMGB1 in Induced Sputum From Patients With Asthma and Chronic Obstructive Pulmonary Disease]. Zhonghua Yi Xue Za Zhi. 2011 Nov 15;91(42):2981-4. PubMed PMID: 22333024.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Levels of HMGB1 in induced sputum from patients with asthma and chronic obstructive pulmonary disease]. AU - Cheng,Zhe, AU - Kang,Yan, AU - Wu,Qiu-ge, AU - Dai,Ling-ling, AU - Song,Yong-na, AU - Xia,Jie, AU - Si,Ji-ming, AU - Chen,Chun-yan, PY - 2012/2/16/entrez PY - 2012/2/16/pubmed PY - 2012/8/3/medline SP - 2981 EP - 4 JF - Zhonghua yi xue za zhi JO - Zhonghua Yi Xue Za Zhi VL - 91 IS - 42 N2 - OBJECTIVE: To explore the relationship between the sputum levels of high mobility group protein B1 (HMGB1) and airway inflammation in bronchial asthma and chronic obstructive pulmonary disease (COPD) patients. METHODS: A total of 57 patients with persistent asthma [per Global Initiative for Asthma (GINA) guidelines], 30 patients with stable COPD [stratified by Global Initiative for COPD (GOLD) status] and 20 control subjects were recruited. After completing an asthma control questionnaire, spirometry was performed before sputum induction. The ratio of forced expiratory volume in the first second (FEV(1))/predictive value (FEV(1)%Pre) and neutrophil differential count in induced sputum were recorded. The concentrations of HMGB1 in the supernatant of sputum were measured by ELISA (enzyme-linked immunosorbent assay). RESULTS: The sputum concentrations of HMGB1 in the asthmatics and COPD patients were significantly higher than those of the control subjects [(291 ± 55) and (511 ± 39) vs (61 ± 5) ng/L, all P < 0.01]. And they were significantly negatively correlated with FEV(1)%Pre in all subjects. The levels of HMGB1 in induced sputum of COPD patients were significantly higher than those of asthmatics and healthy controls (P < 0.01). No significant difference existed in the levels of HMGB1 between patients with eosinophilic and noneosinophilic asthma [(290 ± 55) vs (292 ± 54) ng/L, P > 0.05]. The HMGB1 levels with COPD stage II and stage III were significantly higher than those with stage I [(526 ± 29) and (541 ± 29) vs (471 ± 18) ng/L]. The differences of sputum neutrophil percentage were statistically significant in mild, moderate and severe asthma [(27 ± 2)%, (36 ± 4)%, (49 ± 4)%]. And the sputum levels of HMGB1 were significantly higher in the patients with moderate and severe asthma [(312 ± 14) vs (347 ± 11) ng/L]. And the levels of HMGB1 in asthmatic and COPD patients were positively correlated with neutrophil percentage. According to the multivariate analysis, neutrophil percentage and FEV(1)%Pre were independent predictors of sputum HMGB1, but not smoking, age, gender and eosinophilic percentage. CONCLUSION: HMGB1 may contribute to airway inflammation through its higher expression in bronchial asthma and COPD patients. SN - 0376-2491 UR - https://www.unboundmedicine.com/medline/citation/22333024/[Levels_of_HMGB1_in_induced_sputum_from_patients_with_asthma_and_chronic_obstructive_pulmonary_disease]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&amp;issn=0376-2491&amp;year=2011&amp;vol=91&amp;issue=42&amp;fpage=2981 DB - PRIME DP - Unbound Medicine ER -