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[Chemopreventive effect of boswellic acid and curcumin on 7,12-dimethyl benzanthracene-induced hamster cheek pouch carcinogenesis].
Zhonghua Kou Qiang Yi Xue Za Zhi. 2011 Nov; 46(11):678-83.ZK

Abstract

OBJECTIVE

To evaluate the chemopreventive effects of boswellic acid and curcumin on 7,12-dimethyl benzanthracene(DMBA)-induced oral carcinogenesis in the hamster cheek pouch model.

METHODS

Male Syrian golden hamsters (6 - 8 weeks old, 80 - 130 g in weight) were randomly divided into seven groups, with group A serving as the untreated negative control. The left cheek pouch of the remaining hamsters was topically treated with 0.5% DMBA in mineral oil three times a week for 6 weeks. They were then randomized to six groups with group B serving as a positive control and receiving no further treatment. Groups C-G were treated topically with 5, 10 mg/L boswellic acid, 5, 10 µmol/L curcumin, or the combination of 5 mg/L boswellic acid and 5 µmol/L curcumin three times per week for 18 weeks. The animals were injected with bromodeoxyuridine intraperitoneally at 50 mg/kg 2 h prior to killing. At the 25 th week all the hamsters were sacrificed and cheek pouch tissue was harvested. One half of the tissue was snap frozen in liquid nitrogen for analysis of arachidonic acid metabolites, and the other half was fixed in 10% phosphate-buffered saline(PBS)-buffered formalin for histopathological examination.

RESULTS

Six-weeks of DMBA followed by 18-weeks of topical application of boswellic acid and curcumin, both boswellic acid (5, 10 mg/L) and curcumin (5, 10 µmol/L) significantly inhibited the incidence from 93.8% to 73.9% (P > 0.05), numbers from 2.19 ± 0.98 to 1.13 ± 0.81 (P < 0.01) and size of visible tumors. Microscopically the incidence of squamous cell carcinoma and BrdU index were also significantly suppressed by boswellic acid and curcumin.

CONCLUSIONS

Both boswellic acid and curcumin were effective in preventing oral carcinogenesis in DMBA-induced hamster cheek pouch model.

Authors+Show Affiliations

Department of Periodontal and Mucosal Disease, Capital Medical University School of Stomatology, Beijng 100050, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

22333308

Citation

Zhang, Xin-yan, et al. "[Chemopreventive Effect of Boswellic Acid and Curcumin On 7,12-dimethyl Benzanthracene-induced Hamster Cheek Pouch Carcinogenesis]." Zhonghua Kou Qiang Yi Xue Za Zhi = Zhonghua Kouqiang Yixue Zazhi = Chinese Journal of Stomatology, vol. 46, no. 11, 2011, pp. 678-83.
Zhang XY, Chen XF, Sun Z, et al. [Chemopreventive effect of boswellic acid and curcumin on 7,12-dimethyl benzanthracene-induced hamster cheek pouch carcinogenesis]. Zhonghua Kou Qiang Yi Xue Za Zhi. 2011;46(11):678-83.
Zhang, X. Y., Chen, X. F., Sun, Z., Miao, C. C., Ge, L. H., & Tian, Z. C. (2011). [Chemopreventive effect of boswellic acid and curcumin on 7,12-dimethyl benzanthracene-induced hamster cheek pouch carcinogenesis]. Zhonghua Kou Qiang Yi Xue Za Zhi = Zhonghua Kouqiang Yixue Zazhi = Chinese Journal of Stomatology, 46(11), 678-83.
Zhang XY, et al. [Chemopreventive Effect of Boswellic Acid and Curcumin On 7,12-dimethyl Benzanthracene-induced Hamster Cheek Pouch Carcinogenesis]. Zhonghua Kou Qiang Yi Xue Za Zhi. 2011;46(11):678-83. PubMed PMID: 22333308.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Chemopreventive effect of boswellic acid and curcumin on 7,12-dimethyl benzanthracene-induced hamster cheek pouch carcinogenesis]. AU - Zhang,Xin-yan, AU - Chen,Xiao-fang, AU - Sun,Zheng, AU - Miao,Cong-cong, AU - Ge,Li-hua, AU - Tian,Zhen-chuan, PY - 2012/2/16/entrez PY - 2012/2/16/pubmed PY - 2015/4/22/medline SP - 678 EP - 83 JF - Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology JO - Zhonghua Kou Qiang Yi Xue Za Zhi VL - 46 IS - 11 N2 - OBJECTIVE: To evaluate the chemopreventive effects of boswellic acid and curcumin on 7,12-dimethyl benzanthracene(DMBA)-induced oral carcinogenesis in the hamster cheek pouch model. METHODS: Male Syrian golden hamsters (6 - 8 weeks old, 80 - 130 g in weight) were randomly divided into seven groups, with group A serving as the untreated negative control. The left cheek pouch of the remaining hamsters was topically treated with 0.5% DMBA in mineral oil three times a week for 6 weeks. They were then randomized to six groups with group B serving as a positive control and receiving no further treatment. Groups C-G were treated topically with 5, 10 mg/L boswellic acid, 5, 10 µmol/L curcumin, or the combination of 5 mg/L boswellic acid and 5 µmol/L curcumin three times per week for 18 weeks. The animals were injected with bromodeoxyuridine intraperitoneally at 50 mg/kg 2 h prior to killing. At the 25 th week all the hamsters were sacrificed and cheek pouch tissue was harvested. One half of the tissue was snap frozen in liquid nitrogen for analysis of arachidonic acid metabolites, and the other half was fixed in 10% phosphate-buffered saline(PBS)-buffered formalin for histopathological examination. RESULTS: Six-weeks of DMBA followed by 18-weeks of topical application of boswellic acid and curcumin, both boswellic acid (5, 10 mg/L) and curcumin (5, 10 µmol/L) significantly inhibited the incidence from 93.8% to 73.9% (P > 0.05), numbers from 2.19 ± 0.98 to 1.13 ± 0.81 (P < 0.01) and size of visible tumors. Microscopically the incidence of squamous cell carcinoma and BrdU index were also significantly suppressed by boswellic acid and curcumin. CONCLUSIONS: Both boswellic acid and curcumin were effective in preventing oral carcinogenesis in DMBA-induced hamster cheek pouch model. SN - 1002-0098 UR - https://www.unboundmedicine.com/medline/citation/22333308/[Chemopreventive_effect_of_boswellic_acid_and_curcumin_on_712_dimethyl_benzanthracene_induced_hamster_cheek_pouch_carcinogenesis]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&amp;issn=1002-0098&amp;year=2011&amp;vol=46&amp;issue=11&amp;fpage=678 DB - PRIME DP - Unbound Medicine ER -