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[Effects of hyperoxia on lung vascular development and expression of angiopoietin-1 in neonatal rat lungs].
Zhonghua Er Ke Za Zhi. 2011 Nov; 49(11):834-8.ZE

Abstract

OBJECTIVE

To study the effects of prolonged 85% oxygen exposure on lung vascular development and the expression of angiopoietin-1 (Ang-1) in the neonatal rat lungs.

METHODS

Ninety-six Sprague-Dawley rat pups were randomly exposed to air (control group) and 85% oxygen (experimental group) 6 hrs after birth. The rats were sacrificed 3, 7 and 14 days after exposure and their lungs were sampled. The lung sections were stained with hematoxylin and eosin for histological evaluation and analysis of vessel volume density. Expressions of angiopoietin-1 (Ang-1) in lung tissue were measured by immunohistochemistry. Expression of Ang-1 protein and mRNA was detected by Western Blot and Real time-PCR.

RESULTS

After being exposed to 85% oxygen for 14 days, lung tissues had pathological changes as "new" bronchopulmonary dysplasia (BPD). The RAC on day 7 and day 14 in experimental group decreased significantly as compared with the control group [(10.55 ± 0.13) vs. (11.74 ± 0.19), (12.47 ± 0.05) vs. (15.03 ± 0.16), P < 0.05]. The X-ray showed that the diameter of lung vessel was much smaller and the vessels had less branches in experimental group compared with the control group on day 14. The vessel volume density on day 14 in experimental group decreased significantly as compared with the control group [(3.55 ± 0.09) vs. (6.03 ± 0.16), P < 0.05]. Immunohistochemistry and Western blotting showed that the expressions of Ang-1 protein on day 7 and day 14 in the experimental group decreased significantly as compared with the control group [(4.27 ± 0.34) vs. (3.10 ± 0.29), P < 0.05, (5.65 ± 0.49) vs. (3.21 ± 0.28), P < 0.01], [(0.88 ± 0.31) vs. (0.41 ± 0.12), P < 0.05, (0.90 ± 0.29) vs. (0.21 ± 0.06), P < 0.01]. The expressions of Ang-1 mRNA on day 7 and day 14 in the experimental group also decreased significantly as compared with the control group [(0.85 ± 0.14) vs. (0.44 ± 0.21), P < 0.05, (0.87 ± 0.24) vs. (0.24 ± 0.05), P < 0.01].

CONCLUSIONS

Prolonged exposure of high concentration of oxygen may cause impairment of lung vascular development by inhibiting expression of Ang-1 in neonatal rats, which is likely to contribute to pathogenesis of BPD.

Authors+Show Affiliations

Department of Pediatrics, Shanghai Jiao Tong University, Shanghai, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

22336306

Citation

Liang, Fei, et al. "[Effects of Hyperoxia On Lung Vascular Development and Expression of Angiopoietin-1 in Neonatal Rat Lungs]." Zhonghua Er Ke Za Zhi = Chinese Journal of Pediatrics, vol. 49, no. 11, 2011, pp. 834-8.
Liang F, Zhu JX, Xia HP, et al. [Effects of hyperoxia on lung vascular development and expression of angiopoietin-1 in neonatal rat lungs]. Zhonghua Er Ke Za Zhi. 2011;49(11):834-8.
Liang, F., Zhu, J. X., Xia, H. P., Li, H. P., & Zhu, L. C. (2011). [Effects of hyperoxia on lung vascular development and expression of angiopoietin-1 in neonatal rat lungs]. Zhonghua Er Ke Za Zhi = Chinese Journal of Pediatrics, 49(11), 834-8.
Liang F, et al. [Effects of Hyperoxia On Lung Vascular Development and Expression of Angiopoietin-1 in Neonatal Rat Lungs]. Zhonghua Er Ke Za Zhi. 2011;49(11):834-8. PubMed PMID: 22336306.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of hyperoxia on lung vascular development and expression of angiopoietin-1 in neonatal rat lungs]. AU - Liang,Fei, AU - Zhu,Jian-xing, AU - Xia,Hong-ping, AU - Li,Hui-ping, AU - Zhu,Lü-chang, PY - 2012/2/17/entrez PY - 2012/2/18/pubmed PY - 2012/5/11/medline SP - 834 EP - 8 JF - Zhonghua er ke za zhi = Chinese journal of pediatrics JO - Zhonghua Er Ke Za Zhi VL - 49 IS - 11 N2 - OBJECTIVE: To study the effects of prolonged 85% oxygen exposure on lung vascular development and the expression of angiopoietin-1 (Ang-1) in the neonatal rat lungs. METHODS: Ninety-six Sprague-Dawley rat pups were randomly exposed to air (control group) and 85% oxygen (experimental group) 6 hrs after birth. The rats were sacrificed 3, 7 and 14 days after exposure and their lungs were sampled. The lung sections were stained with hematoxylin and eosin for histological evaluation and analysis of vessel volume density. Expressions of angiopoietin-1 (Ang-1) in lung tissue were measured by immunohistochemistry. Expression of Ang-1 protein and mRNA was detected by Western Blot and Real time-PCR. RESULTS: After being exposed to 85% oxygen for 14 days, lung tissues had pathological changes as "new" bronchopulmonary dysplasia (BPD). The RAC on day 7 and day 14 in experimental group decreased significantly as compared with the control group [(10.55 ± 0.13) vs. (11.74 ± 0.19), (12.47 ± 0.05) vs. (15.03 ± 0.16), P < 0.05]. The X-ray showed that the diameter of lung vessel was much smaller and the vessels had less branches in experimental group compared with the control group on day 14. The vessel volume density on day 14 in experimental group decreased significantly as compared with the control group [(3.55 ± 0.09) vs. (6.03 ± 0.16), P < 0.05]. Immunohistochemistry and Western blotting showed that the expressions of Ang-1 protein on day 7 and day 14 in the experimental group decreased significantly as compared with the control group [(4.27 ± 0.34) vs. (3.10 ± 0.29), P < 0.05, (5.65 ± 0.49) vs. (3.21 ± 0.28), P < 0.01], [(0.88 ± 0.31) vs. (0.41 ± 0.12), P < 0.05, (0.90 ± 0.29) vs. (0.21 ± 0.06), P < 0.01]. The expressions of Ang-1 mRNA on day 7 and day 14 in the experimental group also decreased significantly as compared with the control group [(0.85 ± 0.14) vs. (0.44 ± 0.21), P < 0.05, (0.87 ± 0.24) vs. (0.24 ± 0.05), P < 0.01]. CONCLUSIONS: Prolonged exposure of high concentration of oxygen may cause impairment of lung vascular development by inhibiting expression of Ang-1 in neonatal rats, which is likely to contribute to pathogenesis of BPD. SN - 0578-1310 UR - https://www.unboundmedicine.com/medline/citation/22336306/[Effects_of_hyperoxia_on_lung_vascular_development_and_expression_of_angiopoietin_1_in_neonatal_rat_lungs]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&amp;issn=0578-1310&amp;year=2011&amp;vol=49&amp;issue=11&amp;fpage=834 DB - PRIME DP - Unbound Medicine ER -