Tags

Type your tag names separated by a space and hit enter

[New shuttle-type vectors for cloning in Escherichia coli and Agrobacterium tumefaciens].
Mol Gen Mikrobiol Virusol 1990; (8):12-5MG

Abstract

The vectors capable of replication in Escherichia coli and Agrobacterium tumefaciens have been constructed on the basis of the plasmid pUB5502. The constructed vectors pVA12, pVA12-2, pVA12-4 contain the mini-replicon and trimethoprim resistance gene (Tp) of a broad host-range plasmid R388 (IncW). The pVA12 vector (8.8 kb) has been constructed by insertion of a kanamycin resistance gene (Km) from the plasmid pUC-4K into a Psti site. It possesses 7 unique restriction sites for XhoI, SmaI, PvuI, PvuII, HindIII, EcoRI, BamHI and the markers for kanamycin and trimethoprim resistance (Km and Tp). The pVA12-2 and pVA12-4 vectors were obtained as a result of changing of the PvuII-EcoI fragment of pVA12 carrying the Tp gene for the PvuII-EcoRI fragment of pBR322 carrying the Tc gene. These plasmids have the same size of 9.7 kb and 8 unique sites for restriction endonucleases XhoI, SmaI, PvuI, PvuII, EcoRI, EcoRV, SalI, BalI and Km and Tc genes. No difference has been registered between the two plasmids by restriction analysis, but pVA12-4 has the dramatically increased copy number in Escherichia coli cells. All three vectors are transferable to Agrobacterium tumefaciens with the same frequencies by transformation or conjugation and do not affect the oncogenicity of pTi.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

rus

PubMed ID

2233784

Citation

Denisova, T S., et al. "[New Shuttle-type Vectors for Cloning in Escherichia Coli and Agrobacterium Tumefaciens]." Molekuliarnaia Genetika, Mikrobiologiia I Virusologiia, 1990, pp. 12-5.
Denisova TS, Raspopova VIu, Avdienko ID, et al. [New shuttle-type vectors for cloning in Escherichia coli and Agrobacterium tumefaciens]. Mol Gen Mikrobiol Virusol. 1990.
Denisova, T. S., Raspopova, V. I. u., Avdienko, I. D., Tikhonenko, T. I., & Chernin, L. S. (1990). [New shuttle-type vectors for cloning in Escherichia coli and Agrobacterium tumefaciens]. Molekuliarnaia Genetika, Mikrobiologiia I Virusologiia, (8), pp. 12-5.
Denisova TS, et al. [New Shuttle-type Vectors for Cloning in Escherichia Coli and Agrobacterium Tumefaciens]. Mol Gen Mikrobiol Virusol. 1990;(8)12-5. PubMed PMID: 2233784.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [New shuttle-type vectors for cloning in Escherichia coli and Agrobacterium tumefaciens]. AU - Denisova,T S, AU - Raspopova,V Iu, AU - Avdienko,I D, AU - Tikhonenko,T I, AU - Chernin,L S, PY - 1990/8/1/pubmed PY - 1990/8/1/medline PY - 1990/8/1/entrez SP - 12 EP - 5 JF - Molekuliarnaia genetika, mikrobiologiia i virusologiia JO - Mol. Gen. Mikrobiol. Virusol. IS - 8 N2 - The vectors capable of replication in Escherichia coli and Agrobacterium tumefaciens have been constructed on the basis of the plasmid pUB5502. The constructed vectors pVA12, pVA12-2, pVA12-4 contain the mini-replicon and trimethoprim resistance gene (Tp) of a broad host-range plasmid R388 (IncW). The pVA12 vector (8.8 kb) has been constructed by insertion of a kanamycin resistance gene (Km) from the plasmid pUC-4K into a Psti site. It possesses 7 unique restriction sites for XhoI, SmaI, PvuI, PvuII, HindIII, EcoRI, BamHI and the markers for kanamycin and trimethoprim resistance (Km and Tp). The pVA12-2 and pVA12-4 vectors were obtained as a result of changing of the PvuII-EcoI fragment of pVA12 carrying the Tp gene for the PvuII-EcoRI fragment of pBR322 carrying the Tc gene. These plasmids have the same size of 9.7 kb and 8 unique sites for restriction endonucleases XhoI, SmaI, PvuI, PvuII, EcoRI, EcoRV, SalI, BalI and Km and Tc genes. No difference has been registered between the two plasmids by restriction analysis, but pVA12-4 has the dramatically increased copy number in Escherichia coli cells. All three vectors are transferable to Agrobacterium tumefaciens with the same frequencies by transformation or conjugation and do not affect the oncogenicity of pTi. SN - 0208-0613 UR - https://www.unboundmedicine.com/medline/citation/2233784/[New_shuttle_type_vectors_for_cloning_in_Escherichia_coli_and_Agrobacterium_tumefaciens]_ DB - PRIME DP - Unbound Medicine ER -