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[Directed cloning of the HLA-B27 gene isolated from a patient with ankylosing spondylitis (Bechterew's disease)].
Mol Gen Mikrobiol Virusol. 1990 AugMG

Abstract

The authors have used the modified method of the direct gene cloning suggested by Nichols et al to isolate HLA-B27 gene from a patient suffering from ankylosing spondylitis. Five restriction enzymes (ClaI, HindIII, SnaBI, PvuI, SalGI) which had no recognition sites within the 6.0 kb EcoRI-BamHI-DNA fragment supposedly containing the HLA-B27 gene have been chosen by blotting-hybridization of the restriction fragments of the patients DNA with HLA-B27-specific probe. The 6.0 +/- 0.5 kb DNA fragments were isolated and cloned after the DNA treatment by 300 micrograms of all of these restriction enzymes. The obtained mini-library containing 280 recombinants has been screened with the use of HLA-B27 specific oligonucleotide probe. The clone PB27-2 has been isolated the restriction map of which is identical to HLA-B27k. The authors are planning to determine the sequence of the isolated gene in order to find a possible structural defect in it.

Authors

Segal VE
No affiliation info available
Benevolenskaia LI
No affiliation info available
Stepanov AI
No affiliation info available

MeSH

Cloning, MolecularDNAGenetic MarkersHLA-B27 AntigenHumansNucleic Acid HybridizationPlasmidsRestriction MappingSpondylitis, Ankylosing

Pub Type(s)

English Abstract
Journal Article

Language

rus

PubMed ID

2233790

Citation

Segal, V E., et al. "[Directed Cloning of the HLA-B27 Gene Isolated From a Patient With Ankylosing Spondylitis (Bechterew's Disease)]." Molekuliarnaia Genetika, Mikrobiologiia I Virusologiia, 1990, pp. 8-11.
Segal VE, Benevolenskaia LI, Stepanov AI. [Directed cloning of the HLA-B27 gene isolated from a patient with ankylosing spondylitis (Bechterew's disease)]. Mol Gen Mikrobiol Virusol. 1990.
Segal, V. E., Benevolenskaia, L. I., & Stepanov, A. I. (1990). [Directed cloning of the HLA-B27 gene isolated from a patient with ankylosing spondylitis (Bechterew's disease)]. Molekuliarnaia Genetika, Mikrobiologiia I Virusologiia, (8), 8-11.
Segal VE, Benevolenskaia LI, Stepanov AI. [Directed Cloning of the HLA-B27 Gene Isolated From a Patient With Ankylosing Spondylitis (Bechterew's Disease)]. Mol Gen Mikrobiol Virusol. 1990;(8)8-11. PubMed PMID: 2233790.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Directed cloning of the HLA-B27 gene isolated from a patient with ankylosing spondylitis (Bechterew's disease)]. AU - Segal,V E, AU - Benevolenskaia,L I, AU - Stepanov,A I, PY - 1990/8/1/pubmed PY - 1990/8/1/medline PY - 1990/8/1/entrez SP - 8 EP - 11 JF - Molekuliarnaia genetika, mikrobiologiia i virusologiia JO - Mol Gen Mikrobiol Virusol IS - 8 N2 - The authors have used the modified method of the direct gene cloning suggested by Nichols et al to isolate HLA-B27 gene from a patient suffering from ankylosing spondylitis. Five restriction enzymes (ClaI, HindIII, SnaBI, PvuI, SalGI) which had no recognition sites within the 6.0 kb EcoRI-BamHI-DNA fragment supposedly containing the HLA-B27 gene have been chosen by blotting-hybridization of the restriction fragments of the patients DNA with HLA-B27-specific probe. The 6.0 +/- 0.5 kb DNA fragments were isolated and cloned after the DNA treatment by 300 micrograms of all of these restriction enzymes. The obtained mini-library containing 280 recombinants has been screened with the use of HLA-B27 specific oligonucleotide probe. The clone PB27-2 has been isolated the restriction map of which is identical to HLA-B27k. The authors are planning to determine the sequence of the isolated gene in order to find a possible structural defect in it. SN - 0208-0613 UR - https://www.unboundmedicine.com/medline/citation/2233790/[Directed_cloning_of_the_HLA_B27_gene_isolated_from_a_patient_with_ankylosing_spondylitis__Bechterew's_disease_]_ DB - PRIME DP - Unbound Medicine ER -