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Analgesic effect of gabapentin in a rat model for chronic constrictive injury.
Chin Med J (Engl). 2011 Dec; 124(24):4304-9.CM

Abstract

BACKGROUND

Gabapentin has been widely and successfully used in the clinic for many neuropathic pain syndromes since last decade, however its analgesic mechanisms are still elusive. Our study was to investigate whether Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) contributes to the analgesic effect of gabapentin on a chronic constriction injury (CCI) model.

METHODS

Gabapentin (2%, 100 mg/kg) or saline (0.5 ml/100 g) was injected intraperitoneally 15 minutes prior to surgery and then every 12 hours from postoperative day 0 - 4 to all rats in control, sham and CCI groups. The analgesic effect of gabapentin was assessed by measuring mechanical allodynia and thermal hyperalgesia of rats. Expression and activation of CaMKII were quantified by reverse-transcriptional polymerase chain reaction and Western blotting.

RESULTS

The analgesic effect of gabapentin on mechanical allodynia and thermal hyperalgesia was significant in the CCI model, with maximal reduction reached on postoperative day 8. Gabapentin decreased the expression of the total CaMKII and phosphorylated CaMKII in CCI rats.

CONCLUSION

The analgesic effect of gabapentin on CCI rats may be related to the decreased expression and phosphorylation of CaMKII in the spinal cord.

Authors+Show Affiliations

Department of Anesthesiology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100710, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22340405

Citation

Ma, Lu-Lu, et al. "Analgesic Effect of Gabapentin in a Rat Model for Chronic Constrictive Injury." Chinese Medical Journal, vol. 124, no. 24, 2011, pp. 4304-9.
Ma LL, Liu W, Huang YG, et al. Analgesic effect of gabapentin in a rat model for chronic constrictive injury. Chin Med J (Engl). 2011;124(24):4304-9.
Ma, L. L., Liu, W., Huang, Y. G., Yang, N., & Zuo, P. P. (2011). Analgesic effect of gabapentin in a rat model for chronic constrictive injury. Chinese Medical Journal, 124(24), 4304-9.
Ma LL, et al. Analgesic Effect of Gabapentin in a Rat Model for Chronic Constrictive Injury. Chin Med J (Engl). 2011;124(24):4304-9. PubMed PMID: 22340405.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analgesic effect of gabapentin in a rat model for chronic constrictive injury. AU - Ma,Lu-Lu, AU - Liu,Wei, AU - Huang,Yu-Guang, AU - Yang,Nan, AU - Zuo,Ping-Ping, PY - 2012/2/21/entrez PY - 2012/2/22/pubmed PY - 2012/6/15/medline SP - 4304 EP - 9 JF - Chinese medical journal JO - Chin Med J (Engl) VL - 124 IS - 24 N2 - BACKGROUND: Gabapentin has been widely and successfully used in the clinic for many neuropathic pain syndromes since last decade, however its analgesic mechanisms are still elusive. Our study was to investigate whether Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) contributes to the analgesic effect of gabapentin on a chronic constriction injury (CCI) model. METHODS: Gabapentin (2%, 100 mg/kg) or saline (0.5 ml/100 g) was injected intraperitoneally 15 minutes prior to surgery and then every 12 hours from postoperative day 0 - 4 to all rats in control, sham and CCI groups. The analgesic effect of gabapentin was assessed by measuring mechanical allodynia and thermal hyperalgesia of rats. Expression and activation of CaMKII were quantified by reverse-transcriptional polymerase chain reaction and Western blotting. RESULTS: The analgesic effect of gabapentin on mechanical allodynia and thermal hyperalgesia was significant in the CCI model, with maximal reduction reached on postoperative day 8. Gabapentin decreased the expression of the total CaMKII and phosphorylated CaMKII in CCI rats. CONCLUSION: The analgesic effect of gabapentin on CCI rats may be related to the decreased expression and phosphorylation of CaMKII in the spinal cord. SN - 2542-5641 UR - https://www.unboundmedicine.com/medline/citation/22340405/Analgesic_effect_of_gabapentin_in_a_rat_model_for_chronic_constrictive_injury_ L2 - https://Insights.ovid.com/pubmed?pmid=22340405 DB - PRIME DP - Unbound Medicine ER -