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Inhibitory effects of Zengshengping fractions on DMBA-induced buccal pouch carcinogenesis in hamsters.
Chin Med J (Engl). 2012 Jan; 125(2):332-7.CM

Abstract

BACKGROUND

Zengshengping (ZSP) tablets had inhibitory effects on oral precancerous lesions by reducing the incidence of oral cancer. However, the severe liver toxicity caused by systemic administration of ZSP limits the long-term use of this anti-cancer drug. The purpose of this study was to evaluate the tumor inhibitory effects due to the topical application of extracts from ZSP, a Chinese herbal drug, on 7, 12-dimethlbenz(a)anthracene (DMBA) induced oral tumors in hamsters. The study also investigated the anti-cancer mechanisms of the ZSP extracts on oral carcinogenesis.

METHODS

DMBA (0.5%) was applied topically to the buccal pouches of Syrian golden hamsters (6 - 8 weeks old) three times per week for six weeks in order to induce the development of oral tumors. Different fractions of ZSP were either applied topically to the oral tumor lesions or fed orally at varying dosages to animals with oral tumors for 18 weeks. Tumor volume was measured by histopathological examination. Tumor cell proliferation was evaluated by counting BrdU labeled cells and by Western blotting for mitogen-activated protein kinase (MAPK) protein levels. The protein levels of apoptosis marker Caspase-3 and regulator Bcl-2 protein were also measured by Western blotting.

RESULTS

Topical application of DMBA to the left pouch of hamsters induced oral tumor formation. Animals treated with DMBA showed a loss in body weight while animals treated with ZSP maintained normal body weights. Both the ZSP n-butanol fraction and water fraction significantly reduced tumor volume by 32.6% (P < 0.01) and 22.9% (P < 0.01) respectively. Topical application of ZSP also markedly decreased the BrdU-positive cell numbers in oral tumor lesions and reduced the expression level of MAPK. In addition, ZSP promoted tumor cell apoptosis by increasing Caspase-3 expression but decreasing Bcl-2 protein production.

CONCLUSION

The n-butanol and water fractions of ZSP are effective at inhibiting tumor cell proliferation and stimulating apoptosis in oral cancer suggesting that these fractions have chemopreventive effects on DMBA induced oral carcinogenesis.

Authors+Show Affiliations

Department of Periodontology & Oral Medicine, Capital Medical University School of Stomatology, Beijing 100050, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22340569

Citation

Guan, Xiao-Bing, et al. "Inhibitory Effects of Zengshengping Fractions On DMBA-induced Buccal Pouch Carcinogenesis in Hamsters." Chinese Medical Journal, vol. 125, no. 2, 2012, pp. 332-7.
Guan XB, Sun Z, Chen XX, et al. Inhibitory effects of Zengshengping fractions on DMBA-induced buccal pouch carcinogenesis in hamsters. Chin Med J (Engl). 2012;125(2):332-7.
Guan, X. B., Sun, Z., Chen, X. X., Wu, H. R., & Zhang, X. Y. (2012). Inhibitory effects of Zengshengping fractions on DMBA-induced buccal pouch carcinogenesis in hamsters. Chinese Medical Journal, 125(2), 332-7.
Guan XB, et al. Inhibitory Effects of Zengshengping Fractions On DMBA-induced Buccal Pouch Carcinogenesis in Hamsters. Chin Med J (Engl). 2012;125(2):332-7. PubMed PMID: 22340569.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibitory effects of Zengshengping fractions on DMBA-induced buccal pouch carcinogenesis in hamsters. AU - Guan,Xiao-Bing, AU - Sun,Zheng, AU - Chen,Xiao-Xin, AU - Wu,Hong-Ru, AU - Zhang,Xin-Yan, PY - 2012/2/21/entrez PY - 2012/2/22/pubmed PY - 2012/7/11/medline SP - 332 EP - 7 JF - Chinese medical journal JO - Chin Med J (Engl) VL - 125 IS - 2 N2 - BACKGROUND: Zengshengping (ZSP) tablets had inhibitory effects on oral precancerous lesions by reducing the incidence of oral cancer. However, the severe liver toxicity caused by systemic administration of ZSP limits the long-term use of this anti-cancer drug. The purpose of this study was to evaluate the tumor inhibitory effects due to the topical application of extracts from ZSP, a Chinese herbal drug, on 7, 12-dimethlbenz(a)anthracene (DMBA) induced oral tumors in hamsters. The study also investigated the anti-cancer mechanisms of the ZSP extracts on oral carcinogenesis. METHODS: DMBA (0.5%) was applied topically to the buccal pouches of Syrian golden hamsters (6 - 8 weeks old) three times per week for six weeks in order to induce the development of oral tumors. Different fractions of ZSP were either applied topically to the oral tumor lesions or fed orally at varying dosages to animals with oral tumors for 18 weeks. Tumor volume was measured by histopathological examination. Tumor cell proliferation was evaluated by counting BrdU labeled cells and by Western blotting for mitogen-activated protein kinase (MAPK) protein levels. The protein levels of apoptosis marker Caspase-3 and regulator Bcl-2 protein were also measured by Western blotting. RESULTS: Topical application of DMBA to the left pouch of hamsters induced oral tumor formation. Animals treated with DMBA showed a loss in body weight while animals treated with ZSP maintained normal body weights. Both the ZSP n-butanol fraction and water fraction significantly reduced tumor volume by 32.6% (P < 0.01) and 22.9% (P < 0.01) respectively. Topical application of ZSP also markedly decreased the BrdU-positive cell numbers in oral tumor lesions and reduced the expression level of MAPK. In addition, ZSP promoted tumor cell apoptosis by increasing Caspase-3 expression but decreasing Bcl-2 protein production. CONCLUSION: The n-butanol and water fractions of ZSP are effective at inhibiting tumor cell proliferation and stimulating apoptosis in oral cancer suggesting that these fractions have chemopreventive effects on DMBA induced oral carcinogenesis. SN - 2542-5641 UR - https://www.unboundmedicine.com/medline/citation/22340569/Inhibitory_effects_of_Zengshengping_fractions_on_DMBA_induced_buccal_pouch_carcinogenesis_in_hamsters_ L2 - https://Insights.ovid.com/pubmed?pmid=22340569 DB - PRIME DP - Unbound Medicine ER -