Tags

Type your tag names separated by a space and hit enter

The effect of matrix extracellular phosphoglycoprotein and its downstream osteogenesis-related gene expression on the proliferation and differentiation of human dental pulp cells.
J Endod. 2012 Mar; 38(3):330-8.JE

Abstract

INTRODUCTION

Matrix extracellular phosphoglycoprotein (MEPE), a new member of the small integrin binding ligand N-glycosylated (SIBLING) family, is believed to play multifunctional roles in regulation of cell signaling, mineral homeostasis, and mineralization.

METHODS

To study how MEPE affects the downstream genes involved in regulation of the proliferation and osteogenesis differentiation of dental pulp cells (DPCs), we explored the proliferation and osteogenesis differentiation capability of DPCs stimulated with recombinant MEPE or transfected with adenoviral-mediated human MEPE gene and used a systematic approach by osteogenesis real-time polymerase chain reaction arrays to profile osteogenesis-related gene expression after MEPE gene transfection.

RESULTS

Our results indicated higher proliferation capability in a time- and dose-dependent pattern by cholecystokinin octapeptide assay, and gene/protein expression of osteogenic markers bone sialoprotein, dentin sialophosphoprotein, osteocalcin, and collagen I were up-regulated dependent on time points showed by real-time polymerase chain reaction and Western blot. Moreover, a total of 3 genes, including enamelin, transforming growth factor-β2, and integrin α2, were significantly up-regulated.

CONCLUSIONS

These results indicated that MEPE appeared to play an important positive role in proliferation and osteogenesis differentiation of DPCs through interaction with downstream signals.

Authors+Show Affiliations

Division of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, Guangdong, China. weixi@mail.sysu.edu.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22341070

Citation

Wei, Xi, et al. "The Effect of Matrix Extracellular Phosphoglycoprotein and Its Downstream Osteogenesis-related Gene Expression On the Proliferation and Differentiation of Human Dental Pulp Cells." Journal of Endodontics, vol. 38, no. 3, 2012, pp. 330-8.
Wei X, Liu L, Zhou X, et al. The effect of matrix extracellular phosphoglycoprotein and its downstream osteogenesis-related gene expression on the proliferation and differentiation of human dental pulp cells. J Endod. 2012;38(3):330-8.
Wei, X., Liu, L., Zhou, X., Zhang, F., & Ling, J. (2012). The effect of matrix extracellular phosphoglycoprotein and its downstream osteogenesis-related gene expression on the proliferation and differentiation of human dental pulp cells. Journal of Endodontics, 38(3), 330-8. https://doi.org/10.1016/j.joen.2011.10.015
Wei X, et al. The Effect of Matrix Extracellular Phosphoglycoprotein and Its Downstream Osteogenesis-related Gene Expression On the Proliferation and Differentiation of Human Dental Pulp Cells. J Endod. 2012;38(3):330-8. PubMed PMID: 22341070.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effect of matrix extracellular phosphoglycoprotein and its downstream osteogenesis-related gene expression on the proliferation and differentiation of human dental pulp cells. AU - Wei,Xi, AU - Liu,Lu, AU - Zhou,Xiaoyan, AU - Zhang,Fang, AU - Ling,Junqi, Y1 - 2011/11/23/ PY - 2011/07/19/received PY - 2011/10/14/revised PY - 2011/10/16/accepted PY - 2012/2/21/entrez PY - 2012/2/22/pubmed PY - 2012/7/21/medline SP - 330 EP - 8 JF - Journal of endodontics JO - J Endod VL - 38 IS - 3 N2 - INTRODUCTION: Matrix extracellular phosphoglycoprotein (MEPE), a new member of the small integrin binding ligand N-glycosylated (SIBLING) family, is believed to play multifunctional roles in regulation of cell signaling, mineral homeostasis, and mineralization. METHODS: To study how MEPE affects the downstream genes involved in regulation of the proliferation and osteogenesis differentiation of dental pulp cells (DPCs), we explored the proliferation and osteogenesis differentiation capability of DPCs stimulated with recombinant MEPE or transfected with adenoviral-mediated human MEPE gene and used a systematic approach by osteogenesis real-time polymerase chain reaction arrays to profile osteogenesis-related gene expression after MEPE gene transfection. RESULTS: Our results indicated higher proliferation capability in a time- and dose-dependent pattern by cholecystokinin octapeptide assay, and gene/protein expression of osteogenic markers bone sialoprotein, dentin sialophosphoprotein, osteocalcin, and collagen I were up-regulated dependent on time points showed by real-time polymerase chain reaction and Western blot. Moreover, a total of 3 genes, including enamelin, transforming growth factor-β2, and integrin α2, were significantly up-regulated. CONCLUSIONS: These results indicated that MEPE appeared to play an important positive role in proliferation and osteogenesis differentiation of DPCs through interaction with downstream signals. SN - 1878-3554 UR - https://www.unboundmedicine.com/medline/citation/22341070/The_effect_of_matrix_extracellular_phosphoglycoprotein_and_its_downstream_osteogenesis_related_gene_expression_on_the_proliferation_and_differentiation_of_human_dental_pulp_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0099-2399(11)01204-0 DB - PRIME DP - Unbound Medicine ER -