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Monophyletic outbreak of Hepatitis A involving HIV-infected men who have sex with men, Rome, Italy 2008-2009.
J Clin Virol. 2012 May; 54(1):26-9.JC

Abstract

BACKGROUND

Outbreaks of Acute Hepatitis A Virus (HAV) among men who have sex with men (MSM) have been reported in Europe and, recently, in Italy. From July 2008 through January 2010, 162 HAV infections were diagnosed at National Institute for Infectious Diseases, Rome, Italy, with high male-to-female ratio (M:F=7.5).

OBJECTIVES

The aim of this study was to characterize viral strains involved in this outbreak.

STUDY DESIGN

The sequences of VP1-2A junction of HAV genome, obtained from 67/97 HAV-RNA-positive samples, were used for phylogenetic analysis.

RESULTS

All but 1 of the HAV sequences were genotype 1A, 1 was genotype 1B. A monophyletic cluster, including 59/66 genotype IA sequences, was identified by phylogenetic analysis. This cluster included also 2 HAV strains isolated in Germany (2007) and France (2008) from MSM, that, in turn, were reported to be genetically correlated to HAV strains circulating in Tuscany in 2008. Among the males harboring an HAV strain belonging to the cluster, 62% reported to be MSM, and 25% were HIV-positive, 2 with acute HIV infection.

CONCLUSION

The outbreak occurred in Rome in 2008-2010, involving high proportion of HIV-infected MSM, is sustained by a monophyletic HAV strain, circulating around the same period also in other European countries. Possible factors favouring HAV spread among HIV-infected persons, such as high risk behavior and prolonged fecal excretion, need to be further elucidated. Timely identification of outbreaks with one or the same source of infection may be helpful to implement preventive measures addressing at risk populations.

Authors+Show Affiliations

Laboratory of Virology, National Institute for Infectious Diseases 'L. Spallanzani', 292 Via Portuense, Rome, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22341552

Citation

Bordi, Licia, et al. "Monophyletic Outbreak of Hepatitis a Involving HIV-infected Men Who Have Sex With Men, Rome, Italy 2008-2009." Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, vol. 54, no. 1, 2012, pp. 26-9.
Bordi L, Rozera G, Scognamiglio P, et al. Monophyletic outbreak of Hepatitis A involving HIV-infected men who have sex with men, Rome, Italy 2008-2009. J Clin Virol. 2012;54(1):26-9.
Bordi, L., Rozera, G., Scognamiglio, P., Minosse, C., Loffredo, M., Antinori, A., Narciso, P., Ippolito, G., Girardi, E., & Capobianchi, M. R. (2012). Monophyletic outbreak of Hepatitis A involving HIV-infected men who have sex with men, Rome, Italy 2008-2009. Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, 54(1), 26-9. https://doi.org/10.1016/j.jcv.2012.01.009
Bordi L, et al. Monophyletic Outbreak of Hepatitis a Involving HIV-infected Men Who Have Sex With Men, Rome, Italy 2008-2009. J Clin Virol. 2012;54(1):26-9. PubMed PMID: 22341552.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Monophyletic outbreak of Hepatitis A involving HIV-infected men who have sex with men, Rome, Italy 2008-2009. AU - Bordi,Licia, AU - Rozera,Gabriella, AU - Scognamiglio,Paola, AU - Minosse,Claudia, AU - Loffredo,Mariarosaria, AU - Antinori,Andrea, AU - Narciso,Pasquale, AU - Ippolito,Giuseppe, AU - Girardi,Enrico, AU - Capobianchi,Maria Rosaria, AU - ,, Y1 - 2012/02/16/ PY - 2011/09/18/received PY - 2011/12/01/revised PY - 2012/01/12/accepted PY - 2012/2/21/entrez PY - 2012/2/22/pubmed PY - 2012/7/26/medline SP - 26 EP - 9 JF - Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology JO - J. Clin. Virol. VL - 54 IS - 1 N2 - BACKGROUND: Outbreaks of Acute Hepatitis A Virus (HAV) among men who have sex with men (MSM) have been reported in Europe and, recently, in Italy. From July 2008 through January 2010, 162 HAV infections were diagnosed at National Institute for Infectious Diseases, Rome, Italy, with high male-to-female ratio (M:F=7.5). OBJECTIVES: The aim of this study was to characterize viral strains involved in this outbreak. STUDY DESIGN: The sequences of VP1-2A junction of HAV genome, obtained from 67/97 HAV-RNA-positive samples, were used for phylogenetic analysis. RESULTS: All but 1 of the HAV sequences were genotype 1A, 1 was genotype 1B. A monophyletic cluster, including 59/66 genotype IA sequences, was identified by phylogenetic analysis. This cluster included also 2 HAV strains isolated in Germany (2007) and France (2008) from MSM, that, in turn, were reported to be genetically correlated to HAV strains circulating in Tuscany in 2008. Among the males harboring an HAV strain belonging to the cluster, 62% reported to be MSM, and 25% were HIV-positive, 2 with acute HIV infection. CONCLUSION: The outbreak occurred in Rome in 2008-2010, involving high proportion of HIV-infected MSM, is sustained by a monophyletic HAV strain, circulating around the same period also in other European countries. Possible factors favouring HAV spread among HIV-infected persons, such as high risk behavior and prolonged fecal excretion, need to be further elucidated. Timely identification of outbreaks with one or the same source of infection may be helpful to implement preventive measures addressing at risk populations. SN - 1873-5967 UR - https://www.unboundmedicine.com/medline/citation/22341552/Monophyletic_outbreak_of_Hepatitis_A_involving_HIV_infected_men_who_have_sex_with_men_Rome_Italy_2008_2009_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1386-6532(12)00015-7 DB - PRIME DP - Unbound Medicine ER -