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Evidence for phosphatidylinositol-3-OH-kinase (PI3-kinase) involvement in Cd-mediated oxidative effects on hemocytes of mussels.
Comp Biochem Physiol C Toxicol Pharmacol. 2012 May; 155(4):587-93.CB

Abstract

This study investigated phosphatidylinositol-3-OH-kinase (PI3-kinase) involvement in the induction of cadmium-mediated oxidative effects on hemocytes of mussel Mytilus galloprovincialis. PI3-kinase was investigated with the use of wortmannin, a specific covalent inhibitor of PI3-kinase. Moreover, phorbol-myristate acetate (PMA), a well-known protein kinase C (PKC)-mediated NADPH oxidase and nitric oxide (NO) synthase stimulator, was also used for elucidating PI3-kinase involvement during the respiratory burst process in challenge hemocytes. According to the results, cells pre-treated with non-toxic concentrations of wortmannin (1 and/or 50 nM, as revealed by neutral red retention assay) for 15 min, showed a significant attenuation of cadmium ability (at concentration of 50 μM) to promote cell death, superoxide anion (O(2)(-)) production, NO generation and lipid peroxidation (in terms of malondialdehyde equivalents). On the other hand, wortmannin-treated cells showed a significant attenuation of PMA ability to induce NO generation but not O(2)(-) production. These findings reveal that PI3-kinase could lead to a PKC-independent induction of NO synthase activity in cells faced with pro-oxidants, such as cadmium, while its activation could be fundamental for the regulation of NAPDH oxidase activity, probably through a PKC-dependent signaling pathway.

Authors+Show Affiliations

Department of Biology, Section of Animal Biology, University of Patras, 26500, Greece.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22342345

Citation

Vouras, Christos, and Stefanos Dailianis. "Evidence for phosphatidylinositol-3-OH-kinase (PI3-kinase) Involvement in Cd-mediated Oxidative Effects On Hemocytes of Mussels." Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, vol. 155, no. 4, 2012, pp. 587-93.
Vouras C, Dailianis S. Evidence for phosphatidylinositol-3-OH-kinase (PI3-kinase) involvement in Cd-mediated oxidative effects on hemocytes of mussels. Comp Biochem Physiol C Toxicol Pharmacol. 2012;155(4):587-93.
Vouras, C., & Dailianis, S. (2012). Evidence for phosphatidylinositol-3-OH-kinase (PI3-kinase) involvement in Cd-mediated oxidative effects on hemocytes of mussels. Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, 155(4), 587-93. https://doi.org/10.1016/j.cbpc.2012.01.009
Vouras C, Dailianis S. Evidence for phosphatidylinositol-3-OH-kinase (PI3-kinase) Involvement in Cd-mediated Oxidative Effects On Hemocytes of Mussels. Comp Biochem Physiol C Toxicol Pharmacol. 2012;155(4):587-93. PubMed PMID: 22342345.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evidence for phosphatidylinositol-3-OH-kinase (PI3-kinase) involvement in Cd-mediated oxidative effects on hemocytes of mussels. AU - Vouras,Christos, AU - Dailianis,Stefanos, Y1 - 2012/02/09/ PY - 2011/11/20/received PY - 2012/01/18/revised PY - 2012/01/31/accepted PY - 2012/2/21/entrez PY - 2012/2/22/pubmed PY - 2012/7/11/medline SP - 587 EP - 93 JF - Comparative biochemistry and physiology. Toxicology & pharmacology : CBP JO - Comp Biochem Physiol C Toxicol Pharmacol VL - 155 IS - 4 N2 - This study investigated phosphatidylinositol-3-OH-kinase (PI3-kinase) involvement in the induction of cadmium-mediated oxidative effects on hemocytes of mussel Mytilus galloprovincialis. PI3-kinase was investigated with the use of wortmannin, a specific covalent inhibitor of PI3-kinase. Moreover, phorbol-myristate acetate (PMA), a well-known protein kinase C (PKC)-mediated NADPH oxidase and nitric oxide (NO) synthase stimulator, was also used for elucidating PI3-kinase involvement during the respiratory burst process in challenge hemocytes. According to the results, cells pre-treated with non-toxic concentrations of wortmannin (1 and/or 50 nM, as revealed by neutral red retention assay) for 15 min, showed a significant attenuation of cadmium ability (at concentration of 50 μM) to promote cell death, superoxide anion (O(2)(-)) production, NO generation and lipid peroxidation (in terms of malondialdehyde equivalents). On the other hand, wortmannin-treated cells showed a significant attenuation of PMA ability to induce NO generation but not O(2)(-) production. These findings reveal that PI3-kinase could lead to a PKC-independent induction of NO synthase activity in cells faced with pro-oxidants, such as cadmium, while its activation could be fundamental for the regulation of NAPDH oxidase activity, probably through a PKC-dependent signaling pathway. SN - 1532-0456 UR - https://www.unboundmedicine.com/medline/citation/22342345/Evidence_for_phosphatidylinositol_3_OH_kinase__PI3_kinase__involvement_in_Cd_mediated_oxidative_effects_on_hemocytes_of_mussels_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1532-0456(12)00021-X DB - PRIME DP - Unbound Medicine ER -