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Oxidative stress parameters and antioxidant response to sublethal waterborne zinc in a euryhaline teleost Fundulus heteroclitus: protective effects of salinity.
Aquat Toxicol. 2012 Apr; 110-111:187-93.AT

Abstract

Zinc is an essential trace metal, but many aspects of its toxicity remain unclear. In this study, we investigated zinc effects on oxidative stress parameters and antioxidant profile in four tissues (gill, liver, kidney, and white muscle) of Fundulus heteroclitus. Possible interactive effects of salinity were also studied. Killifish were exposed to sublethal level (500 μgL(-1)) of waterborne zinc for 96 h in 0% (fresh water), 10% (3.5 ppt), 30% (10.5 ppt) and 100% sea water (35 ppt). Salinity per se had no effect on any parameter in the control groups. Zinc exposure clearly induced oxidative stress, and responses were qualitatively similar amongst different tissues. Salinity acted as a strong protective factor, with the highest levels of reactive oxygen species (ROS) and greatest damage (protein carbonyls, lipid peroxidation as indicated by thiobarbituric acid reactive substances (TBARS) in 0 ppt, the least in 100% sea water (35 ppt), and gradations in between in many of the observed responses. Increases in total oxidative scavenging capacity (TOSC) occurred at higher salinities, correlated with increases in the activities of superoxide dismutase (SOD) and glutathione-S-tranferase (GST), as well as in tissue glutathione (GSH) concentrations. However, TOSC was depleted in zinc-exposed fish at 0 ppt, accompanied by decreases in SOD, GST, GSH, and also catalase (CAT) activity. Our results confirm that sublethal waterborne zinc is an oxidative stressor in fish, and highlight the important protective role of higher salinities in ameliorating the oxidative stress associated with zinc toxicity in this model estuarine teleost.

Authors+Show Affiliations

Dept. of Biology, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4K1, Canada. Vania.Loro@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22343464

Citation

Loro, Vania Lucia, et al. "Oxidative Stress Parameters and Antioxidant Response to Sublethal Waterborne Zinc in a Euryhaline Teleost Fundulus Heteroclitus: Protective Effects of Salinity." Aquatic Toxicology (Amsterdam, Netherlands), vol. 110-111, 2012, pp. 187-93.
Loro VL, Jorge MB, Silva KR, et al. Oxidative stress parameters and antioxidant response to sublethal waterborne zinc in a euryhaline teleost Fundulus heteroclitus: protective effects of salinity. Aquat Toxicol. 2012;110-111:187-93.
Loro, V. L., Jorge, M. B., Silva, K. R., & Wood, C. M. (2012). Oxidative stress parameters and antioxidant response to sublethal waterborne zinc in a euryhaline teleost Fundulus heteroclitus: protective effects of salinity. Aquatic Toxicology (Amsterdam, Netherlands), 110-111, 187-93. https://doi.org/10.1016/j.aquatox.2012.01.012
Loro VL, et al. Oxidative Stress Parameters and Antioxidant Response to Sublethal Waterborne Zinc in a Euryhaline Teleost Fundulus Heteroclitus: Protective Effects of Salinity. Aquat Toxicol. 2012;110-111:187-93. PubMed PMID: 22343464.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Oxidative stress parameters and antioxidant response to sublethal waterborne zinc in a euryhaline teleost Fundulus heteroclitus: protective effects of salinity. AU - Loro,Vania Lucia, AU - Jorge,Mariana Basso, AU - Silva,Kassio Rios da, AU - Wood,Chris M, Y1 - 2012/01/24/ PY - 2011/10/31/received PY - 2012/01/11/revised PY - 2012/01/16/accepted PY - 2012/2/21/entrez PY - 2012/2/22/pubmed PY - 2012/7/20/medline SP - 187 EP - 93 JF - Aquatic toxicology (Amsterdam, Netherlands) JO - Aquat. Toxicol. VL - 110-111 N2 - Zinc is an essential trace metal, but many aspects of its toxicity remain unclear. In this study, we investigated zinc effects on oxidative stress parameters and antioxidant profile in four tissues (gill, liver, kidney, and white muscle) of Fundulus heteroclitus. Possible interactive effects of salinity were also studied. Killifish were exposed to sublethal level (500 μgL(-1)) of waterborne zinc for 96 h in 0% (fresh water), 10% (3.5 ppt), 30% (10.5 ppt) and 100% sea water (35 ppt). Salinity per se had no effect on any parameter in the control groups. Zinc exposure clearly induced oxidative stress, and responses were qualitatively similar amongst different tissues. Salinity acted as a strong protective factor, with the highest levels of reactive oxygen species (ROS) and greatest damage (protein carbonyls, lipid peroxidation as indicated by thiobarbituric acid reactive substances (TBARS) in 0 ppt, the least in 100% sea water (35 ppt), and gradations in between in many of the observed responses. Increases in total oxidative scavenging capacity (TOSC) occurred at higher salinities, correlated with increases in the activities of superoxide dismutase (SOD) and glutathione-S-tranferase (GST), as well as in tissue glutathione (GSH) concentrations. However, TOSC was depleted in zinc-exposed fish at 0 ppt, accompanied by decreases in SOD, GST, GSH, and also catalase (CAT) activity. Our results confirm that sublethal waterborne zinc is an oxidative stressor in fish, and highlight the important protective role of higher salinities in ameliorating the oxidative stress associated with zinc toxicity in this model estuarine teleost. SN - 1879-1514 UR - https://www.unboundmedicine.com/medline/citation/22343464/Oxidative_stress_parameters_and_antioxidant_response_to_sublethal_waterborne_zinc_in_a_euryhaline_teleost_Fundulus_heteroclitus:_protective_effects_of_salinity_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-445X(12)00027-6 DB - PRIME DP - Unbound Medicine ER -