Tags

Type your tag names separated by a space and hit enter

Antimicrobial-resistant Shigella infections from Iran: an overlooked problem?
J Antimicrob Chemother 2012; 67(5):1128-33JA

Abstract

OBJECTIVES

In this study, we wanted to assess the level of antimicrobial resistance, the presence of genes encoding resistance to cephalosporins and plasmid-mediated quinolone resistance (PMQR), and genetic relatedness among Shigella isolates obtained from Iranian patients.

METHODS

A total of 44 Shigella isolates were collected from Iranian patients admitted to Milad Hospital, Tehran, Iran, during 2008-10. Of these, 37 were serotyped and characterized by MIC determination. A subset of eight suspected extended-spectrum β-lactamase (ESBL) producers (six Shigella sonnei phase II and two Shigella flexneri type 1b) were examined for the presence of genes encoding cephalosporin resistance. The presence of PMQR was assessed in one S. flexneri isolate exhibiting low-level resistance to ciprofloxacin and susceptibility to nalidixic acid. PFGE was performed on 25 S. sonnei phase II isolates.

RESULTS

Of the isolates, 25 (68%) were S. sonnei phase II, with 5 (14%) S. flexneri, 5 (14%) Shigella dysenteriae type 2, and 2 (5%) Shigella boydii type 2. Resistance to at least threeclasses of antimicrobials was detected in all species. The presence of bla(CTX-M-15) and the AmpC β-lactamase producer bla(CMY-2) was confirmed in five and one S. sonnei phase II isolates, respectively. One of the two S. flexneri type 1b that contained bla(CTX-M-15) also harboured a qnrS1 gene. PFGE identified sevenPFGE profiles; the main cluster included 15 of the strains, suggesting low genetic diversity between isolates or the presence of an endemic clone in Iran.

CONCLUSIONS

This is the first known description of ESBL-producing and AmpC β-lactamase-producing Shigella and of PMQR Shigella in Iran. The emergence of CTX-15, CMY-2 and qnrS1 genes may compromise the treatment of shigellosis. Strategies to minimize the spread of ESBL-producing and AmpC-β-lactamase-producing Shigella should be implemented.

Authors+Show Affiliations

Research Center for Gastroenterology and Liver Disease, Shahid Beheshti University, Medical Sciences, Tehran, Iran.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22345385

Citation

Tajbakhsh, Mercedeh, et al. "Antimicrobial-resistant Shigella Infections From Iran: an Overlooked Problem?" The Journal of Antimicrobial Chemotherapy, vol. 67, no. 5, 2012, pp. 1128-33.
Tajbakhsh M, García Migura L, Rahbar M, et al. Antimicrobial-resistant Shigella infections from Iran: an overlooked problem? J Antimicrob Chemother. 2012;67(5):1128-33.
Tajbakhsh, M., García Migura, L., Rahbar, M., Svendsen, C. A., Mohammadzadeh, M., Zali, M. R., ... Hendriksen, R. S. (2012). Antimicrobial-resistant Shigella infections from Iran: an overlooked problem? The Journal of Antimicrobial Chemotherapy, 67(5), pp. 1128-33. doi:10.1093/jac/dks023.
Tajbakhsh M, et al. Antimicrobial-resistant Shigella Infections From Iran: an Overlooked Problem. J Antimicrob Chemother. 2012;67(5):1128-33. PubMed PMID: 22345385.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antimicrobial-resistant Shigella infections from Iran: an overlooked problem? AU - Tajbakhsh,Mercedeh, AU - García Migura,Lourdes, AU - Rahbar,Mohammad, AU - Svendsen,Christina Aaby, AU - Mohammadzadeh,Mona, AU - Zali,Mohammad Reza, AU - Aarestrup,Frank M, AU - Hendriksen,Rene S, Y1 - 2012/02/17/ PY - 2012/2/21/entrez PY - 2012/2/22/pubmed PY - 2012/7/18/medline SP - 1128 EP - 33 JF - The Journal of antimicrobial chemotherapy JO - J. Antimicrob. Chemother. VL - 67 IS - 5 N2 - OBJECTIVES: In this study, we wanted to assess the level of antimicrobial resistance, the presence of genes encoding resistance to cephalosporins and plasmid-mediated quinolone resistance (PMQR), and genetic relatedness among Shigella isolates obtained from Iranian patients. METHODS: A total of 44 Shigella isolates were collected from Iranian patients admitted to Milad Hospital, Tehran, Iran, during 2008-10. Of these, 37 were serotyped and characterized by MIC determination. A subset of eight suspected extended-spectrum β-lactamase (ESBL) producers (six Shigella sonnei phase II and two Shigella flexneri type 1b) were examined for the presence of genes encoding cephalosporin resistance. The presence of PMQR was assessed in one S. flexneri isolate exhibiting low-level resistance to ciprofloxacin and susceptibility to nalidixic acid. PFGE was performed on 25 S. sonnei phase II isolates. RESULTS: Of the isolates, 25 (68%) were S. sonnei phase II, with 5 (14%) S. flexneri, 5 (14%) Shigella dysenteriae type 2, and 2 (5%) Shigella boydii type 2. Resistance to at least threeclasses of antimicrobials was detected in all species. The presence of bla(CTX-M-15) and the AmpC β-lactamase producer bla(CMY-2) was confirmed in five and one S. sonnei phase II isolates, respectively. One of the two S. flexneri type 1b that contained bla(CTX-M-15) also harboured a qnrS1 gene. PFGE identified sevenPFGE profiles; the main cluster included 15 of the strains, suggesting low genetic diversity between isolates or the presence of an endemic clone in Iran. CONCLUSIONS: This is the first known description of ESBL-producing and AmpC β-lactamase-producing Shigella and of PMQR Shigella in Iran. The emergence of CTX-15, CMY-2 and qnrS1 genes may compromise the treatment of shigellosis. Strategies to minimize the spread of ESBL-producing and AmpC-β-lactamase-producing Shigella should be implemented. SN - 1460-2091 UR - https://www.unboundmedicine.com/medline/citation/22345385/Antimicrobial_resistant_Shigella_infections_from_Iran:_an_overlooked_problem L2 - https://academic.oup.com/jac/article-lookup/doi/10.1093/jac/dks023 DB - PRIME DP - Unbound Medicine ER -