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The effect of composition of different ecotoxicological test media on free and bioavailable copper from CuSO4 and CuO nanoparticles: comparative evidence from a Cu-selective electrode and a Cu-biosensor.
Sensors (Basel). 2011; 11(11):10502-21.S

Abstract

The analysis of (bio)available copper in complex environmental settings, including biological test media, is a challenging task. In this study, we demonstrated the potential of a recombinant Pseudomonas fluorescens-based biosensor for bioavailability analysis of CuSO4 and CuO nanoparticles (nano-CuO) in seventeen different ecotoxicological and microbiologial test media. In parallel, free Cu in these test media was analysed using Cu-ion selective electrode (Cu-ISE). In the case of CuSO4, both free and bioavailable Cu decreased greatly with increasing concentration of organics and phosphates in the tested media. A good correlation between free and bioavailable Cu was observed (r = 0.854, p < 0.01) indicating that the free Cu content in biological test media may be a reasonably good predictor for the toxicity of CuSO4. As a proof, it was demonstrated that when eleven EC50 values for CuSO4 from different organisms in different test media were normalized for the free Cu in these media, the difference in these EC50 values was decreased from 4 to 1.8 orders of magnitude. Thus, toxicity of CuSO4 to these organisms was attributed to the properties of the test media rather than to inherent differences in sensitivity between the test organisms. Differently from CuSO4, the amount of free and bioavailable Cu in nano-CuO spiked media was not significantly correlated with the concentration of organics in the test media. Thus, the speciation of nano-CuO in toxicological test systems was not only determined by the complexation of Cu ions but also by differential dissolution of nano-CuO in different test conditions leading to a new speciation equilibrium. In addition, a substantial fraction of nano-CuO that was not detectable by Cu-ISE (i.e., not present as free Cu-ions) was bioavailable to Cu-biosensor bacteria. Thus, in environmental hazard analysis of (nano) particulate materials, biosensor analysis may be more informative than other analytical techniques. Our results demonstrate that bacterial Cu-biosensors either in combination with other analytical/speciation techniques or on their own, may serve as a rapid (eco)toxicological screening method.

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Authors+Show Affiliations

Käkinen A
Laboratory of Molecular Genetics, National Institute of Chemical Physics and Biophysics, Akadeemia tee 23, Tallinn 12618, Estonia. aleksandr.kakinen@kbfi.ee
Bondarenko O
No affiliation info available
Ivask A
No affiliation info available
Kahru A
No affiliation info available

MeSH

Aliivibrio fischeriAnimalsAnostracaBiological AvailabilityBiosensing TechniquesBuffersChlorophytaCopperCopper SulfateCulture MediaDaphniaEscherichia coliInhibitory Concentration 50Ion-Selective ElectrodesLimit of DetectionLuminescent MeasurementsMaterials TestingMetal NanoparticlesPseudomonas fluorescensSaccharomyces cerevisiaeTetrahymena thermophilaToxicity TestsWater Pollutants, Chemical

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22346655

Citation

Käkinen, Aleksandr, et al. "The Effect of Composition of Different Ecotoxicological Test Media On Free and Bioavailable Copper From CuSO4 and CuO Nanoparticles: Comparative Evidence From a Cu-selective Electrode and a Cu-biosensor." Sensors (Basel, Switzerland), vol. 11, no. 11, 2011, pp. 10502-21.
Käkinen A, Bondarenko O, Ivask A, et al. The effect of composition of different ecotoxicological test media on free and bioavailable copper from CuSO4 and CuO nanoparticles: comparative evidence from a Cu-selective electrode and a Cu-biosensor. Sensors (Basel). 2011;11(11):10502-21.
Käkinen, A., Bondarenko, O., Ivask, A., & Kahru, A. (2011). The effect of composition of different ecotoxicological test media on free and bioavailable copper from CuSO4 and CuO nanoparticles: comparative evidence from a Cu-selective electrode and a Cu-biosensor. Sensors (Basel, Switzerland), 11(11), 10502-21. https://doi.org/10.3390/s111110502
Käkinen A, et al. The Effect of Composition of Different Ecotoxicological Test Media On Free and Bioavailable Copper From CuSO4 and CuO Nanoparticles: Comparative Evidence From a Cu-selective Electrode and a Cu-biosensor. Sensors (Basel). 2011;11(11):10502-21. PubMed PMID: 22346655.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effect of composition of different ecotoxicological test media on free and bioavailable copper from CuSO4 and CuO nanoparticles: comparative evidence from a Cu-selective electrode and a Cu-biosensor. AU - Käkinen,Aleksandr, AU - Bondarenko,Olesja, AU - Ivask,Angela, AU - Kahru,Anne, Y1 - 2011/11/03/ PY - 2011/09/20/received PY - 2011/10/20/revised PY - 2011/10/31/accepted PY - 2012/2/21/entrez PY - 2012/2/22/pubmed PY - 2012/7/13/medline KW - bacteria KW - bioluminescent bioreporter KW - complexation KW - copper-containing nanoparticles KW - ion-selective electrode KW - speciation KW - toxicity SP - 10502 EP - 21 JF - Sensors (Basel, Switzerland) JO - Sensors (Basel) VL - 11 IS - 11 N2 - The analysis of (bio)available copper in complex environmental settings, including biological test media, is a challenging task. In this study, we demonstrated the potential of a recombinant Pseudomonas fluorescens-based biosensor for bioavailability analysis of CuSO4 and CuO nanoparticles (nano-CuO) in seventeen different ecotoxicological and microbiologial test media. In parallel, free Cu in these test media was analysed using Cu-ion selective electrode (Cu-ISE). In the case of CuSO4, both free and bioavailable Cu decreased greatly with increasing concentration of organics and phosphates in the tested media. A good correlation between free and bioavailable Cu was observed (r = 0.854, p < 0.01) indicating that the free Cu content in biological test media may be a reasonably good predictor for the toxicity of CuSO4. As a proof, it was demonstrated that when eleven EC50 values for CuSO4 from different organisms in different test media were normalized for the free Cu in these media, the difference in these EC50 values was decreased from 4 to 1.8 orders of magnitude. Thus, toxicity of CuSO4 to these organisms was attributed to the properties of the test media rather than to inherent differences in sensitivity between the test organisms. Differently from CuSO4, the amount of free and bioavailable Cu in nano-CuO spiked media was not significantly correlated with the concentration of organics in the test media. Thus, the speciation of nano-CuO in toxicological test systems was not only determined by the complexation of Cu ions but also by differential dissolution of nano-CuO in different test conditions leading to a new speciation equilibrium. In addition, a substantial fraction of nano-CuO that was not detectable by Cu-ISE (i.e., not present as free Cu-ions) was bioavailable to Cu-biosensor bacteria. Thus, in environmental hazard analysis of (nano) particulate materials, biosensor analysis may be more informative than other analytical techniques. Our results demonstrate that bacterial Cu-biosensors either in combination with other analytical/speciation techniques or on their own, may serve as a rapid (eco)toxicological screening method. SN - 1424-8220 UR - https://www.unboundmedicine.com/medline/citation/22346655/The_effect_of_composition_of_different_ecotoxicological_test_media_on_free_and_bioavailable_copper_from_CuSO4_and_CuO_nanoparticles:_comparative_evidence_from_a_Cu_selective_electrode_and_a_Cu_biosensor_ L2 - https://www.mdpi.com/resolver?pii=s111110502 DB - PRIME DP - Unbound Medicine ER -